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AB183033

Anti-Scc4 antibody [EPR14390]

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(1 Review)

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(12 Publications)

Rabbit Recombinant Monoclonal SCC4 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 12 publications.

View Alternative Names

KIAA0892, SCC4, MAU2, MAU2 chromatid cohesion factor homolog, MAU-2, Cohesin loading complex subunit SCC4 homolog

4 Images
Flow Cytometry (Intracellular) - Anti-Scc4 antibody [EPR14390] (AB183033)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Scc4 antibody [EPR14390] (AB183033)

Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Scc4 with purified ab183033 at 1/50 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-Scc4 antibody [EPR14390] (AB183033)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Scc4 antibody [EPR14390] (AB183033)

Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Scc4 with purified ab183033 at 1/100 dilution (1.0 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunoprecipitation - Anti-Scc4 antibody [EPR14390] (AB183033)
  • IP

Lab

Immunoprecipitation - Anti-Scc4 antibody [EPR14390] (AB183033)

Purified ab183033 at 1/30 dilution (2μg) immunoprecipitating Scc4 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg.
Lane 2 (+) : ab183033 + HeLa whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab183033 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Scc4 antibody [EPR14390] (ab183033)

Predicted band size: 69 kDa

Observed band size: 69 kDa

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Western blot - Anti-Scc4 antibody [EPR14390] (AB183033)
  • WB

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Western blot - Anti-Scc4 antibody [EPR14390] (AB183033)

All lanes:

Western blot - Anti-Scc4 antibody [EPR14390] (ab183033) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2:

HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate

Lane 3:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate

Lane 4:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

Lane 5:

C6 (Rat glial tumor glial cell) whole cell lysate

Lane 6:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

Lane 7:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 69 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14390

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IP, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/60", "IP-species-notes": "<p><strong>For unpurified use at 1/40 - 1/60.</strong></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/250.</strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p>For unpurified use at 1/90.<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/250.</strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p>For unpurified use at 1/90.<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/250.</strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p>For unpurified use at 1/90.<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Scc4 also known as STAG2 or SCC4 is a subunit of the cohesion complex loading onto chromosomes during the cell division. It has a molecular weight of around 92 kDa. Scc4 is mainly expressed in the nucleoplasm and interacts with other cohesion subunits to help establish sister chromatid cohesion. It forms a complex with other proteins to ensure correct chromosome segregation which is an important process for maintaining genome stability.
Biological function summary

Scc4 plays a role in chromosomal dynamics and is part of the cohesion loader complex which includes Scc2. Scc4's main function involves facilitating the loading and stabilization of the cohesin complex on chromosomes during the S-phase of the cell cycle. Successfully forming the cohesion structure enables physical connection of sister chromatids ensuring they divide properly. This is important for genetic information to pass accurately from cell generation to generation.

Pathways

Scc4 is mostly involved in cell cycle regulation engaging especially in the pathways tied to chromosome segregation and DNA repair. The complex it forms with Scc2 is critical to load cohesins onto chromatin during DNA replication influencing other proteins like Scc1 and Rad21. These proteins coordinate cohesin activity and interaction in the DNA damage repair mechanisms alongside chromosome segregation processes.

Mutations in Scc4's gene have been linked to Cornelia de Lange Syndrome and certain forms of cancer especially acute myeloid leukemia. Its dysfunction can lead to improper chromosomal segregation and mutagenesis contributing to cancer development. The relationship between Scc4 and SMC3 or NIPBL other components of the cohesion complex highlights the interconnectedness of these proteins in both genetic stability and disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in the loading of the cohesin complex on to DNA. Forms a heterodimeric complex (also known as cohesin loading complex) with NIPBL/SCC2 which mediates the loading of the cohesin complex onto chromatin (PubMed : 22628566, PubMed : 28167679). Plays a role in sister chromatid cohesion and normal progression through prometaphase (PubMed : 16682347, PubMed : 16802858).
See full target information MAU2
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 25:5537-5560 PubMed39487368

2024

STAG2 loss in Ewing sarcoma alters enhancer-promoter contacts dependent and independent of EWS::FLI1.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Giménez-Llorente,Ana Cuadrado,María José Andreu,Inmaculada Sanclemente-Alamán,Maria Solé-Ferran,Miriam Rodríguez-Corsino,Ana Losada

Cellular and molecular life sciences : CMLS 81:439 PubMed39453535

2024

The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility.

Applications

Unspecified application

Species

Unspecified reactive species

Marika Foglia,Luca Guarrera,Mami Kurosaki,Giada Andrea Cassanmagnago,Marco Bolis,Matteo Miduri,Anna Cereseto,Alessandro Umbach,Ilaria Craparotta,Maddalena Fratelli,Arianna Vallerga,Gabriela Paroni,Adriana Zanetti,Andrea Vincenzo Cavallaro,Luca Russo,Enrico Garattini,Mineko Terao

Nature communications 15:1579 PubMed38383676

2024

Oncogenic c-Myc induces replication stress by increasing cohesins chromatin occupancy in a CTCF-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Peripolli,Leticia Meneguello,Chiara Perrod,Tanya Singh,Harshil Patel,Sazia T Rahman,Koshiro Kiso,Peter Thorpe,Vincenzo Calvanese,Cosetta Bertoli,Robertus A M de Bruin

Nature structural & molecular biology 30:1286-1294 PubMed37592094

2023

PCNA recruits cohesin loader Scc2 to ensure sister chromatid cohesion.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan Psakhye,Ryotaro Kawasumi,Takuya Abe,Kouji Hirota,Dana Branzei

Genome biology 24:184 PubMed37559123

2023

LAST-seq: single-cell RNA sequencing by direct amplification of single-stranded RNA without prior reverse transcription and second-strand synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Lyu,Chongyi Chen

Genome biology 24:155 PubMed37381036

2023

RAD21 is the core subunit of the cohesin complex involved in directing genome organization.

Applications

Unspecified application

Species

Unspecified reactive species

Yuao Sun,Xin Xu,Wenxue Zhao,Yu Zhang,Keyang Chen,Yongzheng Li,Xiaotian Wang,Mengling Zhang,Boxin Xue,Wanting Yu,Yingping Hou,Chaobin Wang,Wei Xie,Cheng Li,Daochun Kong,Shu Wang,Yujie Sun

eLife 12: PubMed37010886

2023

Cohesin-independent STAG proteins interact with RNA and R-loops and promote complex loading.

Applications

Unspecified application

Species

Unspecified reactive species

Hayley Porter,Yang Li,Maria Victoria Neguembor,Manuel Beltran,Wazeer Varsally,Laura Martin,Manuel Tavares Cornejo,Dubravka Pezić,Amandeep Bhamra,Silvia Surinova,Richard G Jenner,Maria Pia Cosma,Suzana Hadjur

Molecular cancer therapeutics 21:1076-1089 PubMed35439318

2022

A Whole-Genome CRISPR Screen Identifies AHR Loss as a Mechanism of Resistance to a PARP7 Inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Huadong Chen,Morgan E Diolaiti,Patrick C O'Leary,Ajda Rojc,Nevan J Krogan,Minkyu Kim,Alan Ashworth

Life science alliance 4: PubMed34462321

2021

Paralogous synthetic lethality underlies genetic dependencies of the cancer-mutated gene .

Applications

Unspecified application

Species

Unspecified reactive species

Melanie L Bailey,David Tieu,Andrea Habsid,Amy Hin Yan Tong,Katherine Chan,Jason Moffat,Philip Hieter

Cell reports 31:107647 PubMed32433956

2020

MAU2 and NIPBL Variants Impair the Heterodimerization of the Cohesin Loader Subunits and Cause Cornelia de Lange Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Parenti,Farah Diab,Sara Ruiz Gil,Eskeatnaf Mulugeta,Valentina Casa,Riccardo Berutti,Rutger W W Brouwer,Valerie Dupé,Juliane Eckhold,Elisabeth Graf,Beatriz Puisac,Feliciano Ramos,Thomas Schwarzmayr,Macarena Moronta Gines,Thomas van Staveren,Wilfred F J van IJcken,Tim M Strom,Juan Pié,Erwan Watrin,Frank J Kaiser,Kerstin S Wendt
View all publications
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