Rabbit Recombinant Monoclonal SCC4 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 6 publications.
Images
![Immunoprecipitation - Anti-Scc4 antibody [EPR14390] (AB183033), expandable thumbnail](https://content.abcam.com/products/images/scc4-antibody-epr14390-ab183033--immunoprecipitation-img172548.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Scc4 antibody [EPR14390] (AB183033)")
![Western blot - Anti-Scc4 antibody [EPR14390] (AB183033), expandable thumbnail](https://content.abcam.com/products/images/scc4-antibody-epr14390-ab183033--western-blot-img175120.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Scc4 antibody [EPR14390] (AB183033)")
![Immunocytochemistry/ Immunofluorescence - Anti-Scc4 antibody [EPR14390] (AB183033), expandable thumbnail](https://content.abcam.com/products/images/scc4-antibody-epr14390-ab183033--immunocytochemistry-immunofluorescence-img179343.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Scc4 antibody [EPR14390] (AB183033)")
![Flow Cytometry (Intracellular) - Anti-Scc4 antibody [EPR14390] (AB183033), expandable thumbnail](https://content.abcam.com/products/images/scc4-antibody-epr14390-ab183033--flow-cytometry-intracellular-img179863.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Scc4 antibody [EPR14390] (AB183033)")
Publications
Filter by
- Nature structural & molecular biology 30:1286-12942023PCNA recruits cohesin loader Scc2 to ensure sister chromatid cohesion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIvan Psakhye,Ryotaro Kawasumi,Takuya Abe,Kouji Hirota,Dana BranzeiPubMed 37592094
- eLife 12:2023Cohesin-independent STAG proteins interact with RNA and R-loops and promote complex loading.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHayley Porter,Yang Li,Maria Victoria Neguembor,Manuel Beltran,Wazeer Varsally,Laura Martin,Manuel Tavares Cornejo,Dubravka Pezić,Amandeep Bhamra,Silvia Surinova,Richard G Jenner,Maria Pia Cosma,Suzana HadjurPubMed 37010886
- Molecular cancer therapeutics 21:1076-10892022A Whole-Genome CRISPR Screen Identifies AHR Loss as a Mechanism of Resistance to a PARP7 Inhibitor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuadong Chen,Morgan E Diolaiti,Patrick C O'Leary,Ajda Rojc,Nevan J Krogan,Minkyu Kim,Alan AshworthPubMed 35439318
- Cell reports 31:1076472020MAU2 and NIPBL Variants Impair the Heterodimerization of the Cohesin Loader Subunits and Cause Cornelia de Lange Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlaria Parenti,Farah Diab,Sara Ruiz Gil,Eskeatnaf Mulugeta,Valentina Casa,Riccardo Berutti,Rutger W W Brouwer,Valerie Dupé,Juliane Eckhold,Elisabeth Graf,Beatriz Puisac,Feliciano Ramos,Thomas Schwarzmayr,Macarena Moronta Gines,Thomas van Staveren,Wilfred F J van IJcken,Tim M Strom,Juan Pié,Erwan Watrin,Frank J Kaiser,Kerstin S WendtPubMed 32433956
- Cell reports 26:3132-3144.e72019Single-Cell Heterogeneity Analysis and CRISPR Screen Identify Key β-Cell-Specific Disease Genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhou Fang,Chen Weng,Haiyan Li,Ran Tao,Weihua Mai,Xiaoxiao Liu,Leina Lu,Sisi Lai,Qing Duan,Carlos Alvarez,Peter Arvan,Anthony Wynshaw-Boris,Yun Li,Yanxin Pei,Fulai Jin,Yan LiPubMed 30865899
- eLife 7:2018MCM2-7-dependent cohesin loading during S phase promotes sister-chromatid cohesion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGe Zheng et. al.PubMed 29611806
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Tested | Tested |
| Rat | Expected | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 - 1/60 | Notes For unpurified use at 1/40 - 1/60. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes For unpurified use at 1/250. |
Species Rat | Dilution info 1/100 | Notes For unpurified use at 1/250. |
Species Human | Dilution info 1/100 | Notes For unpurified use at 1/250. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes For unpurified use at 1/90.Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info 1/50 | Notes For unpurified use at 1/90.Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info 1/50 | Notes For unpurified use at 1/90.Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target data
Function
Plays an important role in the loading of the cohesin complex on to DNA. Forms a heterodimeric complex (also known as cohesin loading complex) with NIPBL/SCC2 which mediates the loading of the cohesin complex onto chromatin (PubMed:22628566, PubMed:28167679). Plays a role in sister chromatid cohesion and normal progression through prometaphase (PubMed:16682347, PubMed:16802858).
Alternative names
KIAA0892, SCC4, MAU2, MAU2 chromatid cohesion factor homolog, MAU-2, Cohesin loading complex subunit SCC4 homolog
Recommended products
Rabbit Recombinant Monoclonal SCC4 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR14390
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Scc4 also known as STAG2 or SCC4 is a subunit of the cohesion complex loading onto chromosomes during the cell division. It has a molecular weight of around 92 kDa. Scc4 is mainly expressed in the nucleoplasm and interacts with other cohesion subunits to help establish sister chromatid cohesion. It forms a complex with other proteins to ensure correct chromosome segregation which is an important process for maintaining genome stability.
- Biological function summary
Scc4 plays a role in chromosomal dynamics and is part of the cohesion loader complex which includes Scc2. Scc4's main function involves facilitating the loading and stabilization of the cohesin complex on chromosomes during the S-phase of the cell cycle. Successfully forming the cohesion structure enables physical connection of sister chromatids ensuring they divide properly. This is important for genetic information to pass accurately from cell generation to generation.
- Pathways
Scc4 is mostly involved in cell cycle regulation engaging especially in the pathways tied to chromosome segregation and DNA repair. The complex it forms with Scc2 is critical to load cohesins onto chromatin during DNA replication influencing other proteins like Scc1 and Rad21. These proteins coordinate cohesin activity and interaction in the DNA damage repair mechanisms alongside chromosome segregation processes.
- Associated diseases and disorders
Mutations in Scc4's gene have been linked to Cornelia de Lange Syndrome and certain forms of cancer especially acute myeloid leukemia. Its dysfunction can lead to improper chromosomal segregation and mutagenesis contributing to cancer development. The relationship between Scc4 and SMC3 or NIPBL other components of the cohesion complex highlights the interconnectedness of these proteins in both genetic stability and disease pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-Scc4 antibody [EPR14390] (ab183033), expandable thumbnail](https://content.abcam.com/products/images/scc4-antibody-epr14390-ab183033--immunoprecipitation-img172548.jpg "Immunoprecipitation - Anti-Scc4 antibody [EPR14390] (ab183033)")
Immunoprecipitation - Anti-Scc4 antibody [EPR14390] (ab183033)
Purified ab183033 at 1/30 dilution (2μg) immunoprecipitating Scc4 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg.
Lane 2 (+): ab183033 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab183033 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.All lanes: Immunoprecipitation - Anti-Scc4 antibody [EPR14390] (ab183033)
Predicted band size: 69 kDa
Observed band size: 69 kDa
![Western blot - Anti-Scc4 antibody [EPR14390] (ab183033), expandable thumbnail](https://content.abcam.com/products/images/scc4-antibody-epr14390-ab183033--western-blot-img175120.jpg "Western blot - Anti-Scc4 antibody [EPR14390] (ab183033)")
Western blot - Anti-Scc4 antibody [EPR14390] (ab183033)
All lanes: Western blot - Anti-Scc4 antibody [EPR14390] (ab183033) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate
Lane 3: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 5: C6 (Rat glial tumor glial cell) whole cell lysate
Lane 6: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 7: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 69 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Scc4 antibody [EPR14390] (ab183033), expandable thumbnail](https://content.abcam.com/products/images/scc4-antibody-epr14390-ab183033--immunocytochemistry-immunofluorescence-img179343.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Scc4 antibody [EPR14390] (ab183033)")
Immunocytochemistry/ Immunofluorescence - Anti-Scc4 antibody [EPR14390] (ab183033)
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Scc4 with purified ab183033 at 1/100 dilution (1.0 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Flow Cytometry (Intracellular) - Anti-Scc4 antibody [EPR14390] (ab183033), expandable thumbnail](https://content.abcam.com/products/images/scc4-antibody-epr14390-ab183033--flow-cytometry-intracellular-img179863.jpg "Flow Cytometry (Intracellular) - Anti-Scc4 antibody [EPR14390] (ab183033)")
Flow Cytometry (Intracellular) - Anti-Scc4 antibody [EPR14390] (ab183033)
Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Scc4 with purified ab183033 at 1/50 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com