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Rabbit Recombinant Monoclonal SCC4 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 6 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Tested
Tested
Rat
Expected
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/30 - 1/60

Notes

For unpurified use at 1/40 - 1/60.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

For unpurified use at 1/250.

Species

Rat

Dilution info

1/100

Notes

For unpurified use at 1/250.

Species

Human

Dilution info

1/100

Notes

For unpurified use at 1/250.

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

For unpurified use at 1/90.ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Rat

Dilution info

1/50

Notes

For unpurified use at 1/90.ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info

1/50

Notes

For unpurified use at 1/90.ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target data

Function

Plays an important role in the loading of the cohesin complex on to DNA. Forms a heterodimeric complex (also known as cohesin loading complex) with NIPBL/SCC2 which mediates the loading of the cohesin complex onto chromatin (PubMed:28167679, PubMed:22628566). Plays a role in sister chromatid cohesion and normal progression through prometaphase (PubMed:16802858, PubMed:16682347).

Alternative names

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Rabbit Recombinant Monoclonal SCC4 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR14390

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

Scc4 plays a role in chromosomal dynamics and is part of the cohesion loader complex which includes Scc2. Scc4's main function involves facilitating the loading and stabilization of the cohesin complex on chromosomes during the S-phase of the cell cycle. Successfully forming the cohesion structure enables physical connection of sister chromatids ensuring they divide properly. This is important for genetic information to pass accurately from cell generation to generation.

Activity summary

Scc4 also known as STAG2 or SCC4 is a subunit of the cohesion complex loading onto chromosomes during the cell division. It has a molecular weight of around 92 kDa. Scc4 is mainly expressed in the nucleoplasm and interacts with other cohesion subunits to help establish sister chromatid cohesion. It forms a complex with other proteins to ensure correct chromosome segregation which is an important process for maintaining genome stability.

Pathways

Scc4 is mostly involved in cell cycle regulation engaging especially in the pathways tied to chromosome segregation and DNA repair. The complex it forms with Scc2 is critical to load cohesins onto chromatin during DNA replication influencing other proteins like Scc1 and Rad21. These proteins coordinate cohesin activity and interaction in the DNA damage repair mechanisms alongside chromosome segregation processes.

Associated diseases and disorders

Mutations in Scc4's gene have been linked to Cornelia de Lange Syndrome and certain forms of cancer especially acute myeloid leukemia. Its dysfunction can lead to improper chromosomal segregation and mutagenesis contributing to cancer development. The relationship between Scc4 and SMC3 or NIPBL other components of the cohesion complex highlights the interconnectedness of these proteins in both genetic stability and disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-Scc4 antibody [EPR14390] (ab183033), expandable thumbnail

    Immunoprecipitation - Anti-Scc4 antibody [EPR14390] (ab183033)

    Purified ab183033 at 1/30 dilution (2μg) immunoprecipitating Scc4 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg.
    Lane 2 (+): ab183033 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab183033 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Scc4 antibody [EPR14390] (AB183033)

    Predicted band size: 69 kDa

    Observed band size: 69 kDa

  • Western blot - Anti-Scc4 antibody [EPR14390] (ab183033), expandable thumbnail

    Western blot - Anti-Scc4 antibody [EPR14390] (ab183033)

    All lanes: Western blot - Anti-Scc4 antibody [EPR14390] (AB183033) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate

    Lane 3: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate

    Lane 4: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

    Lane 5: C6 (Rat glial tumor glial cell) whole cell lysate

    Lane 6: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

    Lane 7: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 69 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Scc4 antibody [EPR14390] (ab183033), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Scc4 antibody [EPR14390] (ab183033)

    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Scc4 with purified ab183033 at 1/100 dilution (1.0 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-Scc4 antibody [EPR14390] (ab183033), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Scc4 antibody [EPR14390] (ab183033)

    Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Scc4 with purified ab183033 at 1/50 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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