Anti-Scc4 antibody [EPR14390] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Scc4 antibody [EPR14390] - BSA and Azide free (AB250646)

This data was developed using ab183033, the same antibody clone in a different buffer formulation. Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Scc4 with purified ab183033 at 1/50 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Scc4 antibody [EPR14390] - BSA and Azide free (AB250646)

This data was developed using ab183033, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Scc4 with purified ab183033 at 1/100 dilution (1.0 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IP

Lab

Immunoprecipitation - Anti-Scc4 antibody [EPR14390] - BSA and Azide free (AB250646)

This data was developed using ab183033, the same antibody clone in a different buffer formulation.
Purified ab183033 at 1/30 dilution (2μg) immunoprecipitating Scc4 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg.
Lane 2 (+) : ab183033 + HeLa whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab183033 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Scc4 antibody [EPR14390] (<a href='/en-us/products/primary-antibodies/scc4-antibody-epr14390-ab183033'>ab183033</a>)

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

• WB

Unknown

Western blot - Anti-Scc4 antibody [EPR14390] - BSA and Azide free (AB250646)

All lanes:

Western blot - Anti-Scc4 antibody [EPR14390] (<a href='/en-us/products/primary-antibodies/scc4-antibody-epr14390-ab183033'>ab183033</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2:

HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate

Lane 3:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate

Lane 4:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

Lane 5:

C6 (Rat glial tumor glial cell) whole cell lysate

Lane 6:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

Lane 7:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 69 kDa

false