AB234666

Anti-SCDGFB/PDGF-D antibody

Name: Anti-SCDGFB/PDGF-D antibody (ab234666) | Abcam
Brand: Abcam Limited
SKU: AB234666
Price: 525 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal SCDGFB/PDGF-D antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Pdgfd.

View Alternative Names

IEGF, SCDGFB, MSTP036, UNQ1899/PRO4345, PDGFD, Platelet-derived growth factor D, PDGF-D, Iris-expressed growth factor, Spinal cord-derived growth factor B, SCDGF-B

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-SCDGFB/PDGF-D antibody (AB234666)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SCDGFB/PDGF-D antibody (AB234666)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SCDGFB/PDGF-D using ab234666 at a dilution of 1/100 in ICC/IF. Secondary antibody is an Alexa Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L).

Supplier Data

Western blot - Anti-SCDGFB/PDGF-D antibody (AB234666)

All lanes:

Western blot - Anti-SCDGFB/PDGF-D antibody (ab234666) at 1/500 dilution

Lane 1:

A549 (Human lung carcinoma cell line) whole cell lysate

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Pdgfd.

Q925I7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCDGFB also known as PDGF-D is a member of the platelet-derived growth factor family. It plays a critical role in cell proliferation and differentiation. The molecular mass of PDGF-D is approximately 47 kDa. PDGF-D is expressed in various tissues including the heart kidney and vascular endothelial cells reflecting its involvement in diverse physiological processes.

Biological function summary

This growth factor facilitates angiogenesis and tissue development. PDGF-D is secreted as a homodimer and becomes active following proteolytic cleavage. This activated form then binds to the PDGF receptor-beta which is a part of a large protein complex involved in signaling cascades. These interactions underline its significant contribution to cellular signaling and vascular development.

Pathways

The involvement of PDGF-D in the PI3K/Akt and MAPK pathways is evident. These pathways play significant roles in regulating cell growth and survival. PDGF-D interacts with related proteins like PI3K and Akt in these pathways orchestrating cellular responses necessary for growth and survival. It highlights the protein's importance in maintaining normal cellular function and response to external signals.

PDGF-D has implications in cardiovascular disease and cancer. Its overexpression can lead to abnormal cell growth contributing to atherosclerosis and tumorigenesis. PDGF-D is associated with VEGF in promoting angiogenesis and fibroblast growth which can exacerbate these conditions. Understanding these links can help in developing targeted therapies for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix (By similarity).

See full target information PDGFD

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:1700 PubMed39725699

2024

MSC-derived exosomal circMYO9B accelerates diabetic wound healing by promoting angiogenesis through the hnRNPU/CBL/KDM1A/VEGFA axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Hongbo Xu,Bichen Xue,Lian Liu,Yulin Tang,Zhichao Wang,Kai Yao

eLife 12: PubMed37010266

2023

Single-cell RNA-seq of heart reveals intercellular communication drivers of myocardial fibrosis in diabetic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Xinqi Lou,Yingjie Zha,Yinyin Qin,Jun Zha,Lei Hong,Zhanli Xie,Shudi Yang,Chen Wang,Jianzhong An,Zhenhao Zhang,Shigang Qiao

Aging 13:5967-5985 PubMed33591946

2021

Transfer of lncRNA UCA1 by hUCMSCs-derived exosomes protects against hypoxia/reoxygenation injury through impairing miR-143-targeted degradation of Bcl-2.

Applications

Unspecified application

Species

Unspecified reactive species

Liwei Diao,Qinghua Zhang

Cancers 12: PubMed32899998

2020

Potential Role of PDGFRβ-Associated THBS4 in Colorectal Cancer Development.

Applications

Unspecified application

Species

Unspecified reactive species

Min Seob Kim,Hyun Seok Choi,Moxin Wu,JiYeon Myung,Eui Joong Kim,Yong Sung Kim,Seungil Ro,Se Eun Ha,Allison Bartlett,Lai Wei,Han-Seung Ryu,Suck Chei Choi,Won Cheol Park,Keun Young Kim,Moon Young Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SCDGFB/PDGF-D antibody

Immunocytochemistry/ Immunofluorescence - Anti-SCDGFB/PDGF-D antibody (AB234666)

Western blot - Anti-SCDGFB/PDGF-D antibody (AB234666)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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