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AB197087

Anti-SCEL antibody

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(2 Publications)

Rabbit Polyclonal SCEL antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SCEL aa 450 to C-terminus.

View Alternative Names

Sciellin, SCEL

2 Images
Western blot - Anti-SCEL antibody (AB197087)
  • WB

Supplier Data

Western blot - Anti-SCEL antibody (AB197087)

All lanes:

Western blot - Anti-SCEL antibody (ab197087) at 1/500 dilution

Lane 1:

A549 lysate

Lane 2:

7721 lysate

Lane 3:

B16 lysate

Lane 4:

SK-HEP-1 lysate

Lane 5:

SW620 lysate

Predicted band size: 78 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCEL antibody (AB197087)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCEL antibody (AB197087)

Immunohistochemical analysis of formalin/PFA-fixed, paraffin-embedded esophageal cancer tissue sections labeling SCEL with ab197087 at a 1/100 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SCEL aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95171

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCEL also known as sciellin is a part of the protein family found in the cornified envelope of the skin. It weighs about 135 kDa and exists mostly in stratified squamous epithelia. You can find SCEL in tissues such as the epidermis esophagus and in other keratinizing epithelium. SCEL plays a role in the structural integrity and resilience of these tissues due to its position near the inner surface of the plasma membrane.
Biological function summary

SCEL contributes to the proper formation and maintenance of the epidermal barrier. It is a component of the cornified envelope a multiprotein structure critical for the skin's barrier function. The formation involves cross-linking with other proteins like involucrin and filaggrin through the action of enzymes such as transglutaminases. SCEL's presence supports the mechanical stability and protective role of keratinized tissues.

Pathways

SCEL fits into the keratinization process which is important for protective barrier formation in skin. SCEL plays a part in the assembly of the cornified envelope interacting alongside involucrin loricrin and small proline-rich proteins. Another pathway involves the regulation of skin hydration through protein interactions in the epidermal differentiation complex. Proteins like filaggrin work closely with SCEL in these pathways to maintain skin integrity.

Abnormal SCEL expression or function associates with skin conditions such as ichthyosis and psoriasis. These disorders often involve disrupted epidermal barrier function linking SCEL with other proteins involved in keratinocyte differentiation and cornification. For instance mutations or dysregulation in SCEL or its pathway components can lead to defective skin barrier and inflammatory skin disorders with possible involvement of proteins like filaggrin in the pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function in the assembly or regulation of proteins in the cornified envelope. The LIM domain may be involved in homotypic or heterotypic associations and may function to localize sciellin to the cornified envelope.
See full target information SCEL
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed39898547

2025

The short conserved region-2 of LARP4 interacts with ribosome-associated RACK1 and promotes translation.

Applications

Unspecified application

Species

Unspecified reactive species

Amitabh Ranjan,Sandy Mattijssen,Nithin Charlly,Isabel Cruz Gallardo,Leah F Pitman,Jennifer C Coleman,Maria R Conte,Richard J Maraia

Molecular carcinogenesis 63:701-713 PubMed38411346

2024

Sciellin promotes the development and progression of thyroid cancer through the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Haohao Guo,Ziyang Wang,Keyu Yin,Runsheng Ma,Yifei Zhang,Fanxiang Yin,Hongqiang Li,Detao Yin
View all publications
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