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AB64677

Anti-SCF antibody

5

(4 Reviews)

|

(33 Publications)

Rabbit Polyclonal SCF antibody. Suitable for ICC/IF, IHC-P, WB and reacts with African green monkey, Human, Recombinant full length protein samples. Cited in 33 publications.

View Alternative Names

MGF, SCF, KITLG, Kit ligand, Mast cell growth factor, Stem cell factor, c-Kit ligand

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCF antibody (AB64677)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCF antibody (AB64677)

IHC image of SCF antibody staining in a section of formalin-fixed paraffin-embedded normal human colon* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab64677, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Immunocytochemistry/ Immunofluorescence - Anti-SCF antibody (AB64677)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SCF antibody (AB64677)

ab64677 staining SCF in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab64677 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 4% paraformaldehyde (10 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Western blot - Anti-SCF antibody (AB64677)
  • WB

Ap

Western blot - Anti-SCF antibody (AB64677)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab64677 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-SCF antibody (ab64677) at 1 µg/mL

All lanes:

SCF Recombinant Protein at 0.1 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

Predicted band size: 30 kDa

Observed band size: 55 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, African green monkey

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Recent retest of the latest lot of this antibody have shown that this product cannot be longer guaranteed in WB using cell lines and tissue lysates expressing the endogenous protein only. Instead batches have passed in WB using recombinant SCF lysate.

Replenishment batches of our polyclonal antibody, ab64677 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab52603.

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Alternative Products

Primary Antibodies

AB52603

Anti-SCF antibody [EP665Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SCF antibody [EP665Y] (AB52603)

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Primary Antibodies

AB247320

Anti-SCF antibody [EP665Y] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SCF antibody [EP665Y] - BSA and Azide free (AB247320)

  • 0
  • 0
Primary Antibodies

AB215092

Alexa Fluor® 594 Anti-SCF antibody [EP665Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-SCF antibody [EP665Y] (AB215092)

  • 5
  • 1

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB195045

Anti-PAX6 antibody [EPR15858]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] (AB195045)

  • 5
  • 9
Primary Antibodies

AB101318

Anti-Zfp281/ZNF281 antibody

Western blot - Anti-Zfp281/ZNF281 antibody (AB101318)

  • 5
  • 2
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "African green monkey": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Goat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Rhesus monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Stem Cell Factor (SCF) also known as kit ligand c-kit ligand or KITLG is a critical protein involved in cell signaling. It usually appears as a 30 kDa glycoprotein. SCF is widely expressed in various tissues including the bone marrow brain and fetal liver. Its major mechanical function is to bind to the c-KIT receptor triggering various intracellular pathways that influence cell proliferation survival and differentiation. Aside from its other names SCF in mouse plasma is often monitored including in assays like c-kit ligand ELISA.
Biological function summary

SCF acts as an indispensable growth factor promoting hematopoiesis and melanogenesis. It plays a significant role in the regulation of hematopoietic stem cells and mast cells. SCF and its receptor c-KIT form a complex that activates signaling pathways essential for these processes. This protein is also involved in gametogenesis by aiding the proliferation and survival of primordial germ cells. SCF's action is not limited to mammals as studies demonstrate its activity in other species like mouse SCF.

Pathways

SCF impacts multiple important biological systems by engaging with c-KIT. The SCF/c-KIT interaction contributes to the MAPK/ERK pathway influencing cell division and growth. It also plays a role in the PI3K/AKT pathway that governs cell survival. Beyond its direct association with c-KIT SCF's involvement in these pathways connects it to several other proteins like RAS and PI3K which are important for signal transduction.

SCF has associations with several pathological conditions. Dysregulation of the SCF/c-KIT pathway can lead to disorders like mastocytosis and certain leukemias. The overactivation or mutations in c-KIT are often implicated in these diseases due to its cooperative action with SCF. Additionally aberrant SCF signaling can contribute to the progression of gastrointestinal stromal tumors (GIST) where c-KIT has known relevance. Understanding SCF's role can therefore provide insights into diagnostic and therapeutic strategies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
See full target information KITLG
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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Journal of cosmetic dermatology 24:e70443 PubMed40916844

2025

Efficacy of Intense Pulsed Light AOPT-LTL Technique in the Treatment of Melasma: An In Vivo and Clinical Study.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Xinlan Wang,Li Gu,Zhinan Shi,Zhiyi Xu,Siqi Shen,Liqun Gu,Lin Chen,Linling Ju,Chenglong Jin,Bingrong Zhou,Hui Hua

Scientific reports 15:19950 PubMed40481048

2025

CircRNA_1809 promotes the osteogenic differentiation of bone marrow mesenchymal stem cells through miR-370-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Yidan Xu,Ningning Liu,Mengjie Xu,Refeina Tuerxun,Mireyeti Shahatibieke,Yuan Yao

Communications biology 8:148 PubMed39885308

2025

Claudin-11 regulates immunological barrier formation and spermatogonial proliferation through stem cell factor.

Applications

Unspecified application

Species

Unspecified reactive species

Taichi Sugawara,Kayoko Sonoda,Nattapran Chompusri,Kazuhiro Noguchi,Seiji Okada,Mikio Furuse,Tomohiko Wakayama

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2402393 PubMed39229959

2024

Sleep Deprivation Triggers the Excessive Activation of Ovarian Primordial Follicles via β2 Adrenergic Receptor Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lichun Weng,Hanqing Hong,Qinyu Zhang,Chengqi Xiao,Qiuwan Zhang,Qian Wang,Ju Huang,Dongmei Lai

Nature communications 15:5791 PubMed38987295

2024

Bioengineered niches that recreate physiological extracellular matrix organisation to support long-term haematopoietic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah Donnelly,Ewan Ross,Yinbo Xiao,Rio Hermantara,Aqeel F Taqi,W Sebastian Doherty-Boyd,Jennifer Cassels,Penelope M Tsimbouri,Karen M Dunn,Jodie Hay,Annie Cheng,R M Dominic Meek,Nikhil Jain,Christopher West,Helen Wheadon,Alison M Michie,Bruno Peault,Adam G West,Manuel Salmeron-Sanchez,Matthew J Dalby

Proceedings of the National Academy of Sciences of the United States of America 121:e2312724121 PubMed38315849

2024

A massive alteration of gene expression in undescended testicles of dogs and the association of variants with cryptorchidism.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Stachowiak,Joanna Nowacka-Woszuk,Alicja Szabelska-Beresewicz,Joanna Zyprych-Walczak,Paulina Krzeminska,Oskar Sosinski,Tomasz Nowak,Marek Switonski

Development (Cambridge, England) 150: PubMed36861441

2023

Sertoli cells are the source of stem cell factor for spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Jacky Peng,Xinyu Thomas Tang,Hui Sophie Shu,Wenjie Dong,Hongfang Shao,Bo O Zhou

Frontiers in pharmacology 13:796380 PubMed35431956

2022

Stimulation of C-Kit Retinal Progenitor Cells by Stem Cell Factor Confers Protection Against Retinal Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Chen,Shanshan Li,Xiaoli Liu,Jingjie Zhao,Lanting Wu,Ran You,Yanling Wang

Frontiers in immunology 13:794974 PubMed35140718

2022

Autocrine/Paracrine Loop Between SCF/c-Kit Mast Cells Promotes Cutaneous Melanoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Tiziana Annese,Roberto Tamma,Mariella Bozza,Alfredo Zito,Domenico Ribatti

Current issues in molecular biology 44:483-497 PubMed35723319

2022

The Transcriptional Cell Atlas of Testis Development in Sheep at Pre-Sexual Maturity.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wu,Tingting Guo,Jianye Li,Chune Niu,Weibo Sun,Shaohua Zhu,Hongchang Zhao,Guoyan Qiao,Mei Han,Xue He,Zengkui Lu,Chao Yuan,Jianlin Han,Jianbin Liu,Bohui Yang,Yaojing Yue
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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