JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB52603

Anti-SCF antibody [EP665Y]

4

(6 Reviews)

|

(23 Publications)

Rabbit Recombinant Monoclonal SCF antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide - Human samples. Cited in 23 publications.

View Alternative Names

MGF, SCF, KITLG, Kit ligand, Mast cell growth factor, Stem cell factor, c-Kit ligand

4 Images
Flow Cytometry (Intracellular) - Anti-SCF antibody [EP665Y] (AB52603)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SCF antibody [EP665Y] (AB52603)

Overlay histogram showing MCF-7 cells stained with ab52603 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52603 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1μg/1x106 cells) used under the same conditions. Acquisition of >5000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCF antibody [EP665Y] (AB52603)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCF antibody [EP665Y] (AB52603)

Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue using ab52603 at a dilution of 1/100-1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-SCF antibody [EP665Y] (AB52603)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SCF antibody [EP665Y] (AB52603)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling SCF with purified ab52603 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).

Western blot - Anti-SCF antibody [EP665Y] (AB52603)
  • WB

Unknown

Western blot - Anti-SCF antibody [EP665Y] (AB52603)

All lanes:

Western blot - Anti-SCF antibody [EP665Y] (ab52603) at 1/10000 dilution

All lanes:

recombinant protein (5ng)

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 30 kDa

Observed band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP665Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20 - 1/50", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Synthetic peptide - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Stem Cell Factor (SCF) also known as kit ligand c-kit ligand or KITLG is a critical protein involved in cell signaling. It usually appears as a 30 kDa glycoprotein. SCF is widely expressed in various tissues including the bone marrow brain and fetal liver. Its major mechanical function is to bind to the c-KIT receptor triggering various intracellular pathways that influence cell proliferation survival and differentiation. Aside from its other names SCF in mouse plasma is often monitored including in assays like c-kit ligand ELISA.
Biological function summary

SCF acts as an indispensable growth factor promoting hematopoiesis and melanogenesis. It plays a significant role in the regulation of hematopoietic stem cells and mast cells. SCF and its receptor c-KIT form a complex that activates signaling pathways essential for these processes. This protein is also involved in gametogenesis by aiding the proliferation and survival of primordial germ cells. SCF's action is not limited to mammals as studies demonstrate its activity in other species like mouse SCF.

Pathways

SCF impacts multiple important biological systems by engaging with c-KIT. The SCF/c-KIT interaction contributes to the MAPK/ERK pathway influencing cell division and growth. It also plays a role in the PI3K/AKT pathway that governs cell survival. Beyond its direct association with c-KIT SCF's involvement in these pathways connects it to several other proteins like RAS and PI3K which are important for signal transduction.

SCF has associations with several pathological conditions. Dysregulation of the SCF/c-KIT pathway can lead to disorders like mastocytosis and certain leukemias. The overactivation or mutations in c-KIT are often implicated in these diseases due to its cooperative action with SCF. Additionally aberrant SCF signaling can contribute to the progression of gastrointestinal stromal tumors (GIST) where c-KIT has known relevance. Understanding SCF's role can therefore provide insights into diagnostic and therapeutic strategies for these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
See full target information KITLG
style
left-column

Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2402393 PubMed39229959

2024

Sleep Deprivation Triggers the Excessive Activation of Ovarian Primordial Follicles via β2 Adrenergic Receptor Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lichun Weng,Hanqing Hong,Qinyu Zhang,Chengqi Xiao,Qiuwan Zhang,Qian Wang,Ju Huang,Dongmei Lai

Cell & bioscience 13:217 PubMed38031173

2023

Single-cell analysis unveils activation of mast cells in colorectal cancer microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyu Xie,Liaoran Niu,Gaozan Zheng,Kunli Du,Songchen Dai,Ruikai Li,Hanjun Dan,Lili Duan,Hongze Wu,Guangming Ren,Xinyu Dou,Fan Feng,Jian Zhang,Jianyong Zheng

Cell cycle (Georgetown, Tex.) 22:291-302 PubMed36258646

2022

Oct4 promoted proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) in colon cancer cells by activating the SCF/c-Kit signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqian Bu,Yulong Liu,Lin Wang,Zhengzheng Yan,Guo Xin,Wen Su

Frontiers in oncology 12:974573 PubMed36110967

2022

Combined treatment of marizomib and cisplatin modulates cervical cancer growth and invasion and enhances antitumor potential and .

Applications

Unspecified application

Species

Unspecified reactive species

Ziruizhuo Zhang,Songcheng Zhang,Bingjie Lin,Qixin Wang,Xiaojing Nie,Yonghua Shi

Molecular medicine reports 24: PubMed34368880

2021

Atractylenolide‑1 alleviates gastroparesis in diabetic rats by activating the stem cell factor/c‑kit signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Li,Wen Cao,Xun-Bin Zhang,Xi-Xia Zhang,Chao Gu,Li-Mei Gu,Cheng-Yu Pan,Yao-Zhou Tian,Min Lu

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 24:352-367 PubMed33030616

2020

Apatinib exhibits synergistic effect with pyrotinib and reverses acquired pyrotinib resistance in HER2-positive gastric cancer via stem cell factor/c-kit signaling and its downstream pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Beibei Su,Tingting Huang,Yu Jin,Han Yin,Hong Qiu,Xianglin Yuan

The Journal of investigative dermatology 140:2230-2241.e9 PubMed32234461

2020

Molecular Hydrogen Protects Human Melanocytes from Oxidative Stress by Activating Nrf2 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Fang,Luyan Tang,Guizhen Wang,Jinran Lin,Wanqing Liao,Weihua Pan,Jinhua Xu

Haematologica : PubMed32336682

2020

Stem cell factor Is implicated In microenvironmental interactions and cellular dynamics of chronic lymphocytic leukemia.

Applications

IHC, Flow Cyt, WB

Species

Human, Human, Human

George I Gavriilidis,Stavroula Ntoufa,Nikos Papakonstantinou,Konstantia Kotta,Triantafyllia Koletsa,Elisavet Chartomatsidou,Theodoros Moysiadis,Niki Stavroyianni,Achilles Anagnostopoulos,Eleni Papadaki,Asterios S Tsiftsoglou,Kostas Stamatopoulos

Development (Cambridge, England) 146: PubMed31548215

2019

SCF/SCFR signaling plays an important role in the early morphogenesis and neurogenesis of human embryonic neural retina.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Gong,Xiangyu He,Qiyou Li,Juncai He,Baishijiao Bian,Yijian Li,Linlin Ge,Yuxiao Zeng,Haiwei Xu,Zheng Qin Yin

Stem cell research & therapy 10:270 PubMed31445515

2019

Mesenchymal stem cells restore the sperm motility from testicular torsion-detorsion injury by regulation of glucose metabolism in sperm.

Applications

Unspecified application

Species

Unspecified reactive species

Chi-Hao Hsiao,Andrea Tung-Qian Ji,Chih-Cheng Chang,Ming-Hsien Chien,Liang-Ming Lee,Jennifer Hui-Chun Ho
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB64677

Anti-SCF antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCF antibody (AB64677)

  • 5
  • 4
Primary Antibodies

AB215092

Alexa Fluor® 594 Anti-SCF antibody [EP665Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-SCF antibody [EP665Y] (AB215092)

  • 5
  • 1

Complementary Products

Assay Kits

AB176109

Human SCF ELISA Kit

SimpleStep

ELISA - Human SCF ELISA Kit (AB176109)

  • 0
  • 0
Primary Antibodies

AB32363

Anti-c-Kit antibody [YR145]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (AB32363)

  • 4
  • 10
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB155090

Anti-SDF1 antibody [EPR1216]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-SDF1 antibody [EPR1216] (AB155090)

  • 5
  • 3

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com