Goat Polyclonal SCG10 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human STMN2 aa 1-50.
Images
Publications
Filter by
- Journal of cell science 120:1447-562007The microtubule destabilizer stathmin mediates the development of dendritic arbors in neuronal cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNoriaki Ohkawa et. al.PubMed 17389683
- Neuroscience 146:784-912007Intrastriatal dopamine D1 antagonism dampens neural plasticity in response to motor cortex lesion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesE J H Davis et. al.PubMed 17331653
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human STMN2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q93045
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes A 1 hour primary incubation is recommended for this product. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min). |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Regulator of microtubule stability. When phosphorylated by MAPK8, stabilizes microtubules and consequently controls neurite length in cortical neurons. In the developing brain, negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone (By similarity).
Alternative names
SCG10, SCGN10, STMN2, Stathmin-2, Superior cervical ganglion-10 protein, Protein SCG10
Recommended products
Goat Polyclonal SCG10 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human STMN2 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human STMN2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q93045
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SCG10 also known as Superior Cervical Ganglion 10 or Stathmin-like 2 functions mechanically as a microtubule destabilizing protein. It belongs to a larger family known as the stathmin family and has a molecular mass of approximately 21 kDa. Expression of SCG10 is mostly in the central nervous system with high levels found in neurons particularly during development and growth of axons. This specific distribution suggests a specialized role in neural processes.
- Biological function summary
SCG10 plays an important role in neural growth by promoting microtubule dynamics. Its activity helps to regulate the assembly and disassembly of microtubules essential for maintaining the structure and function of growing neurons. SCG10 is not typically part of a larger complex; instead it acts alone to perform its microtubule-related tasks. This independent action allows for precise control over neural development and regeneration processes.
- Pathways
SCG10 contributes significantly to pathways involving neural development and axonal transport. It frequently participates in the microtubule-dependent pathways that facilitate rapid changes in axonal architecture. Linked proteins such as tubulin and the stathmin family proteins collaborate in these pathways to modulate microtubule stability. This coordination underpins the efficient transport of cellular components necessary for nerve cell function and response.
- Associated diseases and disorders
SCG10 has associations with neurodegenerative conditions and neuroblastoma. Abnormal SCG10 expression or function can influence neuron health and survival potentially contributing to disorders like Alzheimer's disease. Furthermore alteration in SCG10 levels relates to neuroblastoma because of its role in neural cell growth. Its relationship with the protein Tau in neurodegenerative conditions highlights its importance in maintaining neuronal integrity and function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry/ Immunofluorescence - Anti-SCG10 antibody (ab21190)
ab211990 staining TAF9 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab211990 at 1/100 dilution (pseudocolored in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Western blot - Anti-SCG10 antibody (ab21190)
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
All lanes: Western blot - Anti-SCG10 antibody (ab21190) at 1 µg/mL
All lanes: Human Brain lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size: 21 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com