AB21190

Anti-SCG10 antibody

Name: Anti-SCG10 antibody (ab21190) | Abcam
Brand: Abcam Limited
SKU: AB21190

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(2 Publications)

Goat Polyclonal SCG10 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human STMN2 aa 1-50.

View Alternative Names

SCG10, SCGN10, STMN2, Stathmin-2, Superior cervical ganglion-10 protein, Protein SCG10

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-SCG10 antibody (AB21190)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SCG10 antibody (AB21190)

ab211990 staining TAF9 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab211990 at 1/100 dilution (pseudocolored in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Western blot - Anti-SCG10 antibody (AB21190)

Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

All lanes:

Western blot - Anti-SCG10 antibody (ab21190) at 1 µg/mL

All lanes:

Human Brain lysate (RIPA buffer, 35µg total protein per lane).

Predicted band size: 21 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human STMN2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q93045

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min).</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCG10 also known as Superior Cervical Ganglion 10 or Stathmin-like 2 functions mechanically as a microtubule destabilizing protein. It belongs to a larger family known as the stathmin family and has a molecular mass of approximately 21 kDa. Expression of SCG10 is mostly in the central nervous system with high levels found in neurons particularly during development and growth of axons. This specific distribution suggests a specialized role in neural processes.

Biological function summary

SCG10 plays an important role in neural growth by promoting microtubule dynamics. Its activity helps to regulate the assembly and disassembly of microtubules essential for maintaining the structure and function of growing neurons. SCG10 is not typically part of a larger complex; instead it acts alone to perform its microtubule-related tasks. This independent action allows for precise control over neural development and regeneration processes.

Pathways

SCG10 contributes significantly to pathways involving neural development and axonal transport. It frequently participates in the microtubule-dependent pathways that facilitate rapid changes in axonal architecture. Linked proteins such as tubulin and the stathmin family proteins collaborate in these pathways to modulate microtubule stability. This coordination underpins the efficient transport of cellular components necessary for nerve cell function and response.

SCG10 has associations with neurodegenerative conditions and neuroblastoma. Abnormal SCG10 expression or function can influence neuron health and survival potentially contributing to disorders like Alzheimer's disease. Furthermore alteration in SCG10 levels relates to neuroblastoma because of its role in neural cell growth. Its relationship with the protein Tau in neurodegenerative conditions highlights its importance in maintaining neuronal integrity and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulator of microtubule stability. When phosphorylated by MAPK8, stabilizes microtubules and consequently controls neurite length in cortical neurons. In the developing brain, negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone (By similarity).

See full target information STMN2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 120:1447-56 PubMed17389683

2007

The microtubule destabilizer stathmin mediates the development of dendritic arbors in neuronal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Noriaki Ohkawa,Kazuko Fujitani,Eri Tokunaga,Shigeki Furuya,Kaoru Inokuchi

Neuroscience 146:784-91 PubMed17331653

2007

Intrastriatal dopamine D1 antagonism dampens neural plasticity in response to motor cortex lesion.

Applications

Unspecified application

Species

Unspecified reactive species

E J H Davis,C Coyne,T H McNeill

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SCG10 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SCG10 antibody (AB21190)

Western blot - Anti-SCG10 antibody (AB21190)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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