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AB250859

Anti-SCG10 antibody [EPR15286-39] - BSA and Azide free

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Rabbit Recombinant Monoclonal SCG10 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat samples.

View Alternative Names

SCG10, SCGN10, STMN2, Stathmin-2, Superior cervical ganglion-10 protein, Protein SCG10

2 Images
Immunoprecipitation - Anti-SCG10 antibody [EPR15286-39] - BSA and Azide free (AB250859)
  • IP

Supplier Data

Immunoprecipitation - Anti-SCG10 antibody [EPR15286-39] - BSA and Azide free (AB250859)

This data was developed using ab185956, the same antibody clone in a different buffer formulation.

Immunoprecipitation of Human fetal brain lysates using ab185956 (lane 1). Detection of SCG10 utilised ab185956 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. The control utilised PBS instead of Human fetal brain lysates (lane 2).

Based on the sequence analysis, ab185956 recognizes two isoforms with the predicted MWs of 21KDa and 22KDa, respectively.

All lanes:

Immunoprecipitation - Anti-SCG10 antibody [EPR15286-39] (<a href='/en-us/products/primary-antibodies/scg10-antibody-epr15286-39-ab185956'>ab185956</a>)

Predicted band size: 21 kDa

false

Western blot - Anti-SCG10 antibody [EPR15286-39] - BSA and Azide free (AB250859)
  • WB

Supplier Data

Western blot - Anti-SCG10 antibody [EPR15286-39] - BSA and Azide free (AB250859)

This data was developed using ab185956, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SCG10 antibody [EPR15286-39] (<a href='/en-us/products/primary-antibodies/scg10-antibody-epr15286-39-ab185956'>ab185956</a>) at 1/20000 dilution

Lane 1:

Human fetal brain lysates at 10 µg

Lane 2:

SH-SY5Y cell lysates at 10 µg

Lane 3:

Rat brain lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15286-39

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250859 is the carrier-free version of ab185956.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCG10 also known as Superior Cervical Ganglion 10 or Stathmin-like 2 functions mechanically as a microtubule destabilizing protein. It belongs to a larger family known as the stathmin family and has a molecular mass of approximately 21 kDa. Expression of SCG10 is mostly in the central nervous system with high levels found in neurons particularly during development and growth of axons. This specific distribution suggests a specialized role in neural processes.
Biological function summary

SCG10 plays an important role in neural growth by promoting microtubule dynamics. Its activity helps to regulate the assembly and disassembly of microtubules essential for maintaining the structure and function of growing neurons. SCG10 is not typically part of a larger complex; instead it acts alone to perform its microtubule-related tasks. This independent action allows for precise control over neural development and regeneration processes.

Pathways

SCG10 contributes significantly to pathways involving neural development and axonal transport. It frequently participates in the microtubule-dependent pathways that facilitate rapid changes in axonal architecture. Linked proteins such as tubulin and the stathmin family proteins collaborate in these pathways to modulate microtubule stability. This coordination underpins the efficient transport of cellular components necessary for nerve cell function and response.

SCG10 has associations with neurodegenerative conditions and neuroblastoma. Abnormal SCG10 expression or function can influence neuron health and survival potentially contributing to disorders like Alzheimer's disease. Furthermore alteration in SCG10 levels relates to neuroblastoma because of its role in neural cell growth. Its relationship with the protein Tau in neurodegenerative conditions highlights its importance in maintaining neuronal integrity and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulator of microtubule stability. When phosphorylated by MAPK8, stabilizes microtubules and consequently controls neurite length in cortical neurons. In the developing brain, negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone (By similarity).
See full target information STMN2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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