AB111871

Anti-SCGN/Secretagogin antibody

Name: Anti-SCGN/Secretagogin antibody (ab111871) | Abcam
Brand: Abcam Limited
SKU: AB111871
Price: 480 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal SCGN/Secretagogin antibody. Suitable for IHC-Fr, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SCGN aa 50-250.

View Alternative Names

SECRET, SCGN, Secretagogin

4 Images

Immunohistochemistry (Frozen sections) - Anti-SCGN/Secretagogin antibody (AB111871)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SCGN/Secretagogin antibody (AB111871)

Immunohistochemical analysis of frozen sectioned adult mouse retina tissue labeling SCGN with ab111871 at 1/250 dilution.

beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody at a 1/250 dilution. DAPI was used for nuclear staining.

Immunocytochemistry/ Immunofluorescence - Anti-SCGN/Secretagogin antibody (AB111871)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SCGN/Secretagogin antibody (AB111871)

Immunofluorescence analysis of DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min labelling SCGN protein using ab111871 at a 1/500 dilution (Green).
beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody at a 1/500 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SCGN/Secretagogin antibody (AB111871)

Immunohistochemical analysis of frozen sectioned adult mouse cerebellum tissue labeling SCGN with ab111871 at 1/250 dilution.

NF-H, stained by NF-H antibody at a 1/500 dilution. DAPI was used for nuclear staining.
Antigen Retrieval : Citrate buffer, pH 6.0, 10 min

Unknown

Western blot - Anti-SCGN/Secretagogin antibody (AB111871)

12% SDS PAGE

All lanes:

Western blot - Anti-SCGN/Secretagogin antibody (ab111871) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 32 kDa,44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-Fr, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SCGN aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

O76038

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Cow": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab111871 is purified by antigen-affinity chromatography.

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCGN also known as Secretagogin is a calcium-binding protein with a molecular weight of approximately 33 kDa. It belongs to the family of EF-hand superfamily proteins which is known for its role in various cellular processes. Secretagogin predominantly expresses in neuroendocrine tissues such as the pancreas and central nervous system where it binds calcium and possibly modulates its signaling. It has drawn interest due to its distinctive expression pattern and potential role in regulated secretion.

Biological function summary

The role of Secretagogin extends beyond simple calcium binding. It participates in managing cytoskeleton dynamics through its ability to bind calcium impacting processes like vesicle trafficking. Although not part of a standard protein complex Secretagogin interacts with other cytoskeletal proteins and may influence the release of hormones. Its role in neurotransmission and insulin secretion showcases its significance in both the nervous and endocrine systems.

Pathways

Secretagogin interacts within signaling networks such as the calcium-signaling and insulin-secretion pathways. It has established relationships with other proteins like actin influencing cellular architecture and trafficking. Through these pathways Secretagogin appears instrumental in processes involving rapid response times such as neurotransmitter release or hormone secretion often acting in conjunction with or supporting other calcium-binding proteins.

Secretagogin has connections to diabetes and certain neuroendocrine tumors. Its involvement in insulin secretion makes it relevant in the context of diabetes where it might interact with malfunctioning insulin-related proteins. Additionally in neuroendocrine tumors altered Secretagogin expression could be linked with oncogenic processes as it shares pathways with proteins involved in cell-cycle regulation. Understanding these associations can aid in identifying potential therapeutic targets or biomarkers for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information SCGN

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroendocrinology 34:e13109 PubMed35304807

2022

Combined use of Lu-DOTATATE peptide receptor radionuclide therapy and fluzoparib for treatment of well-differentiated neuroendocrine tumors: A preclinical study.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Fu,Fan Qiu,Cati Raluca Stolniceanu,Fei Yu,Shiming Zang,Yili Xiang,Yue Huang,Milovan Matovic,Cipriana Stefanescu,Qiyun Tang,Feng Wang

Science (New York, N.Y.) 374:eabj6641 PubMed34882453

2021

Mouse and human share conserved transcriptional programs for interneuron development.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchao Shi,Mengdi Wang,Da Mi,Tian Lu,Bosong Wang,Hao Dong,Suijuan Zhong,Youqiao Chen,Le Sun,Xin Zhou,Qiang Ma,Zeyuan Liu,Wei Wang,Junjing Zhang,Qian Wu,Oscar Marín,Xiaoqun Wang

The Journal of comparative neurology 526:877-898 PubMed29218729

2017

Structural and molecular heterogeneity of calretinin-expressing interneurons in the rodent and primate striatum.

Applications

Unspecified application

Species

Mouse

Farid N Garas,Eszter Kormann,Rahul S Shah,Federica Vinciati,Yoland Smith,Peter J Magill,Andrew Sharott

eLife 5: PubMed27669410

2016

Secretagogin expression delineates functionally-specialized populations of striatal parvalbumin-containing interneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Farid N Garas,Rahul S Shah,Eszter Kormann,Natalie M Doig,Federica Vinciati,Kouichi C Nakamura,Matthijs C Dorst,Yoland Smith,Peter J Magill,Andrew Sharott

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SCGN/Secretagogin antibody

Immunohistochemistry (Frozen sections) - Anti-SCGN/Secretagogin antibody (AB111871)

Immunocytochemistry/ Immunofluorescence - Anti-SCGN/Secretagogin antibody (AB111871)

Immunohistochemistry (Frozen sections) - Anti-SCGN/Secretagogin antibody (AB111871)

Western blot - Anti-SCGN/Secretagogin antibody (AB111871)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise