Rabbit Polyclonal SCGN/Secretagogin antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SCGN aa 100 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SCGN aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O76038
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Alternative names
SECRET, SCGN, Secretagogin
Recommended products
Rabbit Polyclonal SCGN/Secretagogin antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SCGN aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SCGN aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O76038
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SCGN also known as Secretagogin is a calcium-binding protein with a molecular weight of approximately 33 kDa. It belongs to the family of EF-hand superfamily proteins which is known for its role in various cellular processes. Secretagogin predominantly expresses in neuroendocrine tissues such as the pancreas and central nervous system where it binds calcium and possibly modulates its signaling. It has drawn interest due to its distinctive expression pattern and potential role in regulated secretion.
- Biological function summary
The role of Secretagogin extends beyond simple calcium binding. It participates in managing cytoskeleton dynamics through its ability to bind calcium impacting processes like vesicle trafficking. Although not part of a standard protein complex Secretagogin interacts with other cytoskeletal proteins and may influence the release of hormones. Its role in neurotransmission and insulin secretion showcases its significance in both the nervous and endocrine systems.
- Pathways
Secretagogin interacts within signaling networks such as the calcium-signaling and insulin-secretion pathways. It has established relationships with other proteins like actin influencing cellular architecture and trafficking. Through these pathways Secretagogin appears instrumental in processes involving rapid response times such as neurotransmitter release or hormone secretion often acting in conjunction with or supporting other calcium-binding proteins.
- Associated diseases and disorders
Secretagogin has connections to diabetes and certain neuroendocrine tumors. Its involvement in insulin secretion makes it relevant in the context of diabetes where it might interact with malfunctioning insulin-related proteins. Additionally in neuroendocrine tumors altered Secretagogin expression could be linked with oncogenic processes as it shares pathways with proteins involved in cell-cycle regulation. Understanding these associations can aid in identifying potential therapeutic targets or biomarkers for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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8 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (ab211362)
Immunohistochemical analysis of mouse olfactory bulb tissue labeling SCGN/Secretagogin in the glomerular layer nucleus with ab211362 at a 1/500 dilution, followed by a fluorescent secondary antibody.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (ab211362)
Immunohistochemical analysis of human pancreas tissue labeling SCGN/Secretagogin in the cytoplasm and nucleus of Langerhans islets with ab211362 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-SCGN/Secretagogin antibody (ab211362)
All lanes: Western blot - Anti-SCGN/Secretagogin antibody (ab211362) at 0.4 µg/mL
All lanes: Human cerebellum tissue
Predicted band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (ab211362)
Immunohistochemical analysis of mouse hypothalamus tissue labeling SCGN/Secretagogin in the paraventricular hypothalamic nucleus with ab211362 at a 1/500 dilution, followed by a fluorescent secondary antibody.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (ab211362)
Immunohistochemical analysis of human cerebellum tissue labeling SCGN/Secretagogin in the nucleus of cells in molecular layer with ab211362 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (ab211362)
Immunohistochemical analysis of mouse brain tissue labeling SCGN/Secretagogin in the fasciola cinereum with ab211362 at a 1/500 dilution, followed by a fluorescent secondary antibody.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (ab211362)
Immunohistochemical analysis of human rectum tissue labeling SCGN/Secretagogin in the cytoplasm of peripheral nerve/ganglion with ab211362 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (ab211362)
Immunohistochemical analysis of human Fallopian tube tissue labeling SCGN/Secretagogin with ab211362 at a 1/500 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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