AB211362

Anti-SCGN/Secretagogin antibody

Name: Anti-SCGN/Secretagogin antibody (ab211362) | Abcam
Brand: Abcam Limited
SKU: AB211362

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal SCGN/Secretagogin antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SCGN aa 100 to C-terminus.

View Alternative Names

SECRET, SCGN, Secretagogin

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (AB211362)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (AB211362)

Immunohistochemical analysis of human cerebellum tissue labeling SCGN/Secretagogin in the nucleus of cells in molecular layer with ab211362 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (AB211362)

Immunohistochemical analysis of human Fallopian tube tissue labeling SCGN/Secretagogin with ab211362 at a 1/500 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (AB211362)

Immunohistochemical analysis of human pancreas tissue labeling SCGN/Secretagogin in the cytoplasm and nucleus of Langerhans islets with ab211362 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (AB211362)

Immunohistochemical analysis of mouse hypothalamus tissue labeling SCGN/Secretagogin in the paraventricular hypothalamic nucleus with ab211362 at a 1/500 dilution, followed by a fluorescent secondary antibody.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (AB211362)

Immunohistochemical analysis of mouse olfactory bulb tissue labeling SCGN/Secretagogin in the glomerular layer nucleus with ab211362 at a 1/500 dilution, followed by a fluorescent secondary antibody.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-SCGN/Secretagogin antibody (AB211362)

All lanes:

Western blot - Anti-SCGN/Secretagogin antibody (ab211362) at 0.4 µg/mL

All lanes:

Human cerebellum tissue

Predicted band size: 32 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (AB211362)

Immunohistochemical analysis of human rectum tissue labeling SCGN/Secretagogin in the cytoplasm of peripheral nerve/ganglion with ab211362 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (AB211362)

Immunohistochemical analysis of mouse brain tissue labeling SCGN/Secretagogin in the fasciola cinereum with ab211362 at a 1/500 dilution, followed by a fluorescent secondary antibody.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SCGN aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O76038

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCGN also known as Secretagogin is a calcium-binding protein with a molecular weight of approximately 33 kDa. It belongs to the family of EF-hand superfamily proteins which is known for its role in various cellular processes. Secretagogin predominantly expresses in neuroendocrine tissues such as the pancreas and central nervous system where it binds calcium and possibly modulates its signaling. It has drawn interest due to its distinctive expression pattern and potential role in regulated secretion.

Biological function summary

The role of Secretagogin extends beyond simple calcium binding. It participates in managing cytoskeleton dynamics through its ability to bind calcium impacting processes like vesicle trafficking. Although not part of a standard protein complex Secretagogin interacts with other cytoskeletal proteins and may influence the release of hormones. Its role in neurotransmission and insulin secretion showcases its significance in both the nervous and endocrine systems.

Pathways

Secretagogin interacts within signaling networks such as the calcium-signaling and insulin-secretion pathways. It has established relationships with other proteins like actin influencing cellular architecture and trafficking. Through these pathways Secretagogin appears instrumental in processes involving rapid response times such as neurotransmitter release or hormone secretion often acting in conjunction with or supporting other calcium-binding proteins.

Secretagogin has connections to diabetes and certain neuroendocrine tumors. Its involvement in insulin secretion makes it relevant in the context of diabetes where it might interact with malfunctioning insulin-related proteins. Additionally in neuroendocrine tumors altered Secretagogin expression could be linked with oncogenic processes as it shares pathways with proteins involved in cell-cycle regulation. Understanding these associations can aid in identifying potential therapeutic targets or biomarkers for these diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information SCGN

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 37:35-49 PubMed32399910

2020

Short-form RON (sf-RON) enhances glucose metabolism to promote cell proliferation via activating β-catenin/SIX1 signaling pathway in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ziliang Wang,Yufei Yang,Shuang Hu,Jian He,Zheng Wu,Zihao Qi,Mingzhu Huang,Rujiao Liu,Ying Lin,Cong Tan,Midie Xu,Zhe Zhang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SCGN/Secretagogin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (AB211362)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (AB211362)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (AB211362)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (AB211362)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (AB211362)

Western blot - Anti-SCGN/Secretagogin antibody (AB211362)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (AB211362)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody (AB211362)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise