Rabbit Recombinant Monoclonal SCGN/Secretagogin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (AB248768), expandable thumbnail](https://content.abcam.com/products/images/scgn-secretagogin-antibody-epr7477-bsa-and-azide-free-ab248768--western-blot-img105331.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (AB248768)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (AB248768), expandable thumbnail](https://content.abcam.com/products/images/scgn-secretagogin-antibody-epr7477-bsa-and-azide-free-ab248768--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66562.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (AB248768)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (AB248768), expandable thumbnail](https://content.abcam.com/products/images/scgn-secretagogin-antibody-epr7477-bsa-and-azide-free-ab248768--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114946.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (AB248768)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (AB248768), expandable thumbnail](https://content.abcam.com/products/images/scgn-secretagogin-antibody-epr7477-bsa-and-azide-free-ab248768--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122696.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (AB248768)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (AB248768), expandable thumbnail](https://content.abcam.com/products/images/scgn-secretagogin-antibody-epr7477-bsa-and-azide-free-ab248768--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104416.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (AB248768)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
| Mouse | Predicted | Expected | Not recommended |
| Rat | Predicted | Expected | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Alternative names
SECRET, SCGN, Secretagogin
Recommended products
Rabbit Recombinant Monoclonal SCGN/Secretagogin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7477
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 6.7 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248768 is the carrier-free version of Anti-SCGN/Secretagogin antibody [EPR7477] ab137017.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SCGN also known as Secretagogin is a calcium-binding protein with a molecular weight of approximately 33 kDa. It belongs to the family of EF-hand superfamily proteins which is known for its role in various cellular processes. Secretagogin predominantly expresses in neuroendocrine tissues such as the pancreas and central nervous system where it binds calcium and possibly modulates its signaling. It has drawn interest due to its distinctive expression pattern and potential role in regulated secretion.
- Biological function summary
The role of Secretagogin extends beyond simple calcium binding. It participates in managing cytoskeleton dynamics through its ability to bind calcium impacting processes like vesicle trafficking. Although not part of a standard protein complex Secretagogin interacts with other cytoskeletal proteins and may influence the release of hormones. Its role in neurotransmission and insulin secretion showcases its significance in both the nervous and endocrine systems.
- Pathways
Secretagogin interacts within signaling networks such as the calcium-signaling and insulin-secretion pathways. It has established relationships with other proteins like actin influencing cellular architecture and trafficking. Through these pathways Secretagogin appears instrumental in processes involving rapid response times such as neurotransmitter release or hormone secretion often acting in conjunction with or supporting other calcium-binding proteins.
- Associated diseases and disorders
Secretagogin has connections to diabetes and certain neuroendocrine tumors. Its involvement in insulin secretion makes it relevant in the context of diabetes where it might interact with malfunctioning insulin-related proteins. Additionally in neuroendocrine tumors altered Secretagogin expression could be linked with oncogenic processes as it shares pathways with proteins involved in cell-cycle regulation. Understanding these associations can aid in identifying potential therapeutic targets or biomarkers for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768), expandable thumbnail](https://content.abcam.com/products/images/scgn-secretagogin-antibody-epr7477-bsa-and-azide-free-ab248768--western-blot-img105331.jpg "Western blot - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768)")
Western blot - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768)
This data was developed using Anti-SCGN/Secretagogin antibody [EPR7477] ab137017, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SCGN/Secretagogin antibody [EPR7477] (Anti-SCGN/Secretagogin antibody [EPR7477] ab137017) at 1/1000 dilution
Lane 1: Fetal brain lysate at 10 µg
Lane 2: Human hypothalamus lysate at 10 µg
Lane 3: Fetal midbrain lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768), expandable thumbnail](https://content.abcam.com/products/images/scgn-secretagogin-antibody-epr7477-bsa-and-azide-free-ab248768--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66562.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768)
This data was developed using Anti-SCGN/Secretagogin antibody [EPR7477] ab137017, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human gastric neuroendocrine carcinoma tissue labelling SCGN/Secretagogin with Anti-SCGN/Secretagogin antibody [EPR7477] ab137017 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768), expandable thumbnail](https://content.abcam.com/products/images/scgn-secretagogin-antibody-epr7477-bsa-and-azide-free-ab248768--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114946.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768)
This data was developed using Anti-SCGN/Secretagogin antibody [EPR7477] ab137017, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling SCGN/Secretagogin with Anti-SCGN/Secretagogin antibody [EPR7477] ab137017 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768), expandable thumbnail](https://content.abcam.com/products/images/scgn-secretagogin-antibody-epr7477-bsa-and-azide-free-ab248768--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122696.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768)
This data was developed using Anti-SCGN/Secretagogin antibody [EPR7477] ab137017, the same antibody clone in a different buffer formulation.Immunohistochemical analysis using Anti-SCGN/Secretagogin antibody [EPR7477] ab137017 showing staining in lung small cell undifferentiated carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768), expandable thumbnail](https://content.abcam.com/products/images/scgn-secretagogin-antibody-epr7477-bsa-and-azide-free-ab248768--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104416.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768)
This data was developed using Anti-SCGN/Secretagogin antibody [EPR7477] ab137017, the same antibody clone in a different buffer formulation.Immunohistochemical analysis using Anti-SCGN/Secretagogin antibody [EPR7477] ab137017 showing staining in Lung carcinoid tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.![OI-RD Scanning - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768), expandable thumbnail](https://content.abcam.com/products/images/scgn-secretagogin-antibody-epr7477-bsa-and-azide-free-ab248768--oi-rd-scanning-img85204.jpg "OI-RD Scanning - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768)")
OI-RD Scanning - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
![OI-RD Scanning - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768), expandable thumbnail](https://content.abcam.com/products/images/scgn-secretagogin-antibody-epr7477-bsa-and-azide-free-ab248768--oi-rd-scanning-img85204.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-SCGN/Secretagogin antibody [EPR7477] - BSA and Azide free (ab248768)")
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