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Rabbit Recombinant Monoclonal SCIN antibody. C-terminal. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

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Images

Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (AB199723), expandable thumbnail
  • Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (AB199723), expandable thumbnail
  • Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (AB199723), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCIN antibody [EPR16310] - C-terminal (AB199723), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCIN antibody [EPR16310] - C-terminal (AB199723), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Ca(2+)-dependent actin filament-severing protein that has a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane (PubMed:26365202, PubMed:8547642). Severing activity is inhibited by phosphatidylinositol 4,5-bis-phosphate (PIP2) (By similarity). In vitro, also has barbed end capping and nucleating activities in the presence of Ca(2+). Required for megakaryocyte differentiation, maturation, polyploidization and apoptosis with the release of platelet-like particles (PubMed:11568009). Plays a role in osteoclastogenesis (OCG) and actin cytoskeletal organization in osteoclasts (By similarity). Regulates chondrocyte proliferation and differentiation (By similarity). Inhibits cell proliferation and tumorigenesis. Signaling is mediated by MAPK, p38 and JNK pathways (PubMed:11568009).

Alternative names

KIAA1905, SCIN, Scinderin, Adseverin

Recommended products

Rabbit Recombinant Monoclonal SCIN antibody. C-terminal. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16310
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SCIN also known as scinderin or adseverin is an actin-severing protein involved in cytoskeletal dynamics. This protein has a mass of approximately 80 kDa. SCIN is expressed in a variety of tissues including neural hematopoietic and endocrine systems. Its mechanical function includes binding to filamentous actin and promoting severing which helps in the reorganization of the actin cytoskeleton. This activity is calcium-dependent making SCIN responsive to intracellular calcium levels.

Biological function summary

SCIN influences cellular processes like secretion motility and division. SCIN belongs to the gelsolin/villin family of actin-modulating proteins and often acts within a protein complex to disassemble actin filaments. It regulates exocytosis by modulating actin dynamics near the plasma membrane especially in cells such as chromaffin cells and platelets. Its ability to rearrange actin filaments is important for maintaining cellular homeostasis and responsive adaptation to environmental changes.

Pathways

SCIN is involved in the cytoskeletal signaling and calcium signaling pathways. It plays a role in actin filament dynamics and is related to other actin-binding proteins like gelsolin and cofilin through these pathways. SCIN interacts with phosphatidylinositol 45-bisphosphate (PIP2) and calcium ions which are important for its activity in cellular signaling networks. These pathways optimize how cells react to stimuli by altering their shape and function.

Associated diseases and disorders

SCIN has been linked to neurodegenerative disorders and cancer. Disruptions in SCIN function can contribute to diseases such as Alzheimer’s where abnormal actin dynamics impact neuronal function. SCIN is also associated with certain cancers through its role in cell migration and metastasis. The protein may interact with tau in neurodegenerative disorders and it interacts with proteins like cortactin in cancer progression influencing cellular motility.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723), expandable thumbnail

    Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)

    Blocking/dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723) at 1/10000 dilution

    Lane 1: Human fetal kidney lysate at 20 µg

    Lane 2: HEK293 (Human embryonic kidney) whole cell lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 80 kDa

    Observed band size: 80 kDa

    Exposure time: 30s

  • Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723), expandable thumbnail

    Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)

    Blocking/dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723) at 1/1000 dilution

    Lane 1: Mouse kidney lysate at 10 µg

    Lane 2: Mouse spleen lysate at 10 µg

    Lane 3: Rat kidney lysate at 10 µg

    Lane 4: Rat spleen lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa

    Observed band size: 80 kDa

    Exposure time: 1min

  • Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723), expandable thumbnail

    Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)

    Blocking/dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723) at 1/1000 dilution

    Lane 1: C6 (rat glial tumor cells) whole cell lysate at 10 µg

    Lane 2: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Lane 3: NIH/3T3 (mouse embryo fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa

    Observed band size: 80 kDa

    Exposure time: 5s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)

    Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling SCIN with ab199723 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on Human clear cell carcinoma of kidney tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SCIN with ab199723 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on Human pancreas tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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