AB85799

Anti-Sclerostin antibody

Name: Anti-Sclerostin antibody (ab85799) | Abcam
Brand: Abcam Limited
SKU: AB85799
Price: 505 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(11 Publications)

Rabbit Polyclonal Sclerostin antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 11 publications.

View Alternative Names

UNQ2976/PRO7455/PRO7476, SOST, Sclerostin

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Sclerostin antibody (AB85799)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Sclerostin antibody (AB85799)

ab85799 staining Sclerostin in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab85799 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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Western blot - Anti-Sclerostin antibody (AB85799)

All lanes:

Western blot - Anti-Sclerostin antibody (ab85799) at 1 µg/mL

All lanes:

Human bone tumor tissue lysate - total protein (<a href='/en-us/products/unavailable/human-bone-tumor-tissue-lysate-total-protein-ab29359'>ab29359</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 24 kDa

Observed band size: 150 kDa,28 kDa,55 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sclerostin also known as SOST is a glycoprotein weighing approximately 22 kDa. It is predominantly expressed in osteocytes the most abundant cells found in bone. Sclerostin is an extracellular molecule that plays a role in bone metabolism by inhibiting the Wnt signaling pathway which is important for bone formation. This protein acts as a negative regulator of bone growth by binding to the LRP5/6 receptors. Inhibitors such as 'anti-sclerostin' antibodies have been developed to block its function and promote bone formation.

Biological function summary

Sclerostin inhibits osteoblastic activity and formation by interfering with bone morphogenetic proteins. It doesn’t form part of a large protein complex but interacts directly with cell surface receptors. Its expression decreases in response to mechanical loading on bones which is an important part of regulating bone density and remodeling. By controlling osteoblast function sclerostin contributes to skeletal homeostasis an essential process for maintaining bone strength and integrity.

Pathways

Several mechanisms regulate sclerostin activity. In the canonical Wnt/β-catenin signaling pathway it serves as an important checkpoint that ensures controlled bone formation. Sclerostin acts by preventing the activation of LRP5/6 receptors pivotal components in this pathway. Additionally it shares a regulatory network with dickkopf-1 (DKK1) another Wnt inhibitor both of which have an influence on bone metabolism and development.

Two primary conditions associated with sclerostin are osteoporosis and sclerosteosis. Osteoporosis arises from excessive inhibition of bone formation and is characterized by fragile bones due to decreased bone mass. On the other hand sclerosteosis results from mutations in the SOST gene leading to excessive bone growth. Sclerostin's interaction with proteins like DKK1 clarifies its role in bone density management and highlights its relevance in therapeutic strategies targeting these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.

See full target information SOST

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8282 PubMed40987787

2025

A galanin-positive population of lumbar spinal cord neurons modulates sexual arousal and copulatory behavior in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Constanze Lenschow,Ana Rita P Mendes,Liliana Ferreira,Bertrand Lacoste,Hugo Marques,Nicolas Gutierrez-Castellanos,Camille Quilgars,Sandrine S Bertrand,Susana Q Lima

Progress in orthodontics 25:45 PubMed39581932

2024

Effect of anti-sclerostin antibody on orthodontic tooth movement in ovariectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunna Ahn,Wonse Park,Sung-Hwan Choi,Namki Hong,Jisun Huh,Seoyeon Jung

Cardiovascular diabetology 22:301 PubMed37919715

2023

Cardioprotective function of sclerostin by reducing calcium deposition, proliferation, and apoptosis in human vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sheila González-Salvatierra,Cristina García-Fontana,Jesus Lacal,Francisco Andújar-Vera,Luis Martínez-Heredia,Raquel Sanabria-de la Torre,María Ferrer-Millán,Enrique Moratalla-Aranda,Manuel Muñoz-Torres,Beatriz García-Fontana

Frontiers in cell and developmental biology 11:1250070 PubMed37822871

2023

Mechanical properties of trabeculae and osteocyte morphology change significantly in different areas of the necrotic femoral head.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Cong He,Jia-Qing Tian,Xiao-Ming He,Peng Yang,Tian-Ye Lin,Qing-Wen Zhang,Wei He,Qiu-Shi Wei

JBMR plus 7:e10792 PubMed37701151

2023

Osteocyte-Like Cells Differentiated From Primary Osteoblasts in an Artificial Human Bone Tissue Model.

Applications

Unspecified application

Species

Unspecified reactive species

Arooj Munir,Janne Elin Reseland,Hanna Tiainen,Håvard Jostein Haugen,Pawel Sikorski,Emil Frang Christiansen,Finn Per Reinholt,Unni Syversen,Lene Bergendal Solberg

Frontiers in endocrinology 14:1167734 PubMed37223031

2023

Mature primary human osteocytes in mini organotypic cultures secrete FGF23 and PTH1-34-regulated sclerostin.

Applications

Unspecified application

Species

Unspecified reactive species

Helen J Knowles,Anastasios Chanalaris,Argyro Koutsikouni,Adam P Cribbs,Liam M Grover,Philippa A Hulley

Journal of orthopaedic surgery and research 18:162 PubMed36864451

2023

IL-1β contributes to the secretion of sclerostin by osteocytes and targeting sclerostin promotes spinal fusion at early stages.

Applications

Unspecified application

Species

Unspecified reactive species

Zengxin Jiang,Lixia Jin,Chang Jiang,Zuoqin Yan,Yuanwu Cao

Biology 11: PubMed36552348

2022

Differential Expression of Dickkopf 1 and Periostin in Mouse Strains with High and Low Bone Mass.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Kerschan-Schindl,Victoria Schramek,Maria Butylina,Ursula Föger-Samwald,Peter Pietschmann

Nature communications 13:4241 PubMed35869074

2022

Targeting loop3 of sclerostin preserves its cardiovascular protective action and promotes bone formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Yu,Luyao Wang,Shuaijian Ni,Dijie Li,Jin Liu,Hang Yin Chu,Ning Zhang,Meiheng Sun,Nanxi Li,Qing Ren,Zhenjian Zhuo,Chuanxin Zhong,Duoli Xie,Yongshu Li,Zong-Kang Zhang,Huarui Zhang,Mei Li,Zhenlin Zhang,Lin Chen,Xiaohua Pan,Weibo Xia,Shu Zhang,Aiping Lu,Bao-Ting Zhang,Ge Zhang

Experimental and therapeutic medicine 21:197 PubMed33488806

2021

Shuxuetong injection simultaneously ameliorates dexamethasone-driven vascular calcification and osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Xu,Xiaoguang Liu,Yanqing Li,Hongliang Gao,Tao He,Chunlei Zhang,Wei Hao,Xu Teng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sclerostin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Sclerostin antibody (AB85799)

Western blot - Anti-Sclerostin antibody (AB85799)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise