AB194940

Anti-Sclerostin antibody - C-terminal

Name: Anti-Sclerostin antibody - C-terminal (ab194940) | Abcam
Brand: Abcam Limited
SKU: AB194940
Rating: 5 (1 reviews)

(1 Review)

(1 Publication)

Goat Polyclonal Sclerostin antibody. C-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SOST aa 150 to C-terminus.

View Alternative Names

UNQ2976/PRO7455/PRO7476, SOST, Sclerostin

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sclerostin antibody - C-terminal (AB194940)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sclerostin antibody - C-terminal (AB194940)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Sclerostin with ab194940 at 4 μg/mL using HRP detection.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human SOST aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BQB4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4-6 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab194940 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sclerostin also known as SOST is a glycoprotein weighing approximately 22 kDa. It is predominantly expressed in osteocytes the most abundant cells found in bone. Sclerostin is an extracellular molecule that plays a role in bone metabolism by inhibiting the Wnt signaling pathway which is important for bone formation. This protein acts as a negative regulator of bone growth by binding to the LRP5/6 receptors. Inhibitors such as 'anti-sclerostin' antibodies have been developed to block its function and promote bone formation.

Biological function summary

Sclerostin inhibits osteoblastic activity and formation by interfering with bone morphogenetic proteins. It doesn’t form part of a large protein complex but interacts directly with cell surface receptors. Its expression decreases in response to mechanical loading on bones which is an important part of regulating bone density and remodeling. By controlling osteoblast function sclerostin contributes to skeletal homeostasis an essential process for maintaining bone strength and integrity.

Pathways

Several mechanisms regulate sclerostin activity. In the canonical Wnt/β-catenin signaling pathway it serves as an important checkpoint that ensures controlled bone formation. Sclerostin acts by preventing the activation of LRP5/6 receptors pivotal components in this pathway. Additionally it shares a regulatory network with dickkopf-1 (DKK1) another Wnt inhibitor both of which have an influence on bone metabolism and development.

Two primary conditions associated with sclerostin are osteoporosis and sclerosteosis. Osteoporosis arises from excessive inhibition of bone formation and is characterized by fragile bones due to decreased bone mass. On the other hand sclerosteosis results from mutations in the SOST gene leading to excessive bone growth. Sclerostin's interaction with proteins like DKK1 clarifies its role in bone density management and highlights its relevance in therapeutic strategies targeting these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.

See full target information SOST

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bone 100:80-86 PubMed28119179

2017

The chronic kidney disease - Mineral bone disorder (CKD-MBD): Advances in pathophysiology.

Applications

Unspecified application

Species

Unspecified reactive species

Keith A Hruska,Toshifumi Sugatani,Olga Agapova,Yifu Fang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sclerostin antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sclerostin antibody - C-terminal (AB194940)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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