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AB86465

Anti-Sclerostin antibody [RM0129-4D77]

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(2 Publications)

Rat Monoclonal Sclerostin antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Sost.

View Alternative Names

Sclerostin, Sost

1 Images
Western blot - Anti-Sclerostin antibody [RM0129-4D77] (AB86465)
  • WB

Supplier Data

Western blot - Anti-Sclerostin antibody [RM0129-4D77] (AB86465)

All lanes:

Western blot - Anti-Sclerostin antibody [RM0129-4D77] (ab86465) at 1/500 dilution

All lanes:

Mouse embryonic tissue

Predicted band size: 24 kDa

false

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RM0129-4D77

Isotype

IgG2

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Sost.

Q99P68

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification notes
IgG fraction of cell culture supernatant purified by Protein A/G affinity chromatography and 0.2 µm filtered.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sclerostin also known as SOST is a glycoprotein weighing approximately 22 kDa. It is predominantly expressed in osteocytes the most abundant cells found in bone. Sclerostin is an extracellular molecule that plays a role in bone metabolism by inhibiting the Wnt signaling pathway which is important for bone formation. This protein acts as a negative regulator of bone growth by binding to the LRP5/6 receptors. Inhibitors such as 'anti-sclerostin' antibodies have been developed to block its function and promote bone formation.
Biological function summary

Sclerostin inhibits osteoblastic activity and formation by interfering with bone morphogenetic proteins. It doesn’t form part of a large protein complex but interacts directly with cell surface receptors. Its expression decreases in response to mechanical loading on bones which is an important part of regulating bone density and remodeling. By controlling osteoblast function sclerostin contributes to skeletal homeostasis an essential process for maintaining bone strength and integrity.

Pathways

Several mechanisms regulate sclerostin activity. In the canonical Wnt/β-catenin signaling pathway it serves as an important checkpoint that ensures controlled bone formation. Sclerostin acts by preventing the activation of LRP5/6 receptors pivotal components in this pathway. Additionally it shares a regulatory network with dickkopf-1 (DKK1) another Wnt inhibitor both of which have an influence on bone metabolism and development.

Two primary conditions associated with sclerostin are osteoporosis and sclerosteosis. Osteoporosis arises from excessive inhibition of bone formation and is characterized by fragile bones due to decreased bone mass. On the other hand sclerosteosis results from mutations in the SOST gene leading to excessive bone growth. Sclerostin's interaction with proteins like DKK1 clarifies its role in bone density management and highlights its relevance in therapeutic strategies targeting these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
See full target information Sost
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular medicine (Berlin, Germany) 101:607-620 PubMed37121919

2023

SOST gene suppression stimulates osteocyte Wnt/β-catenin signaling to prevent bone resorption and attenuates particle-induced osteolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zixue Jiao,Hao Chai,Shendong Wang,Chunguang Sun,Qun Huang,Wei Xu

Development (Cambridge, England) 146: PubMed31511252

2019

Developmentally regulated expression is robust to TAD perturbations.

Applications

Unspecified application

Species

Unspecified reactive species

Iain Williamson,Lauren Kane,Paul S Devenney,Ilya M Flyamer,Eve Anderson,Fiona Kilanowski,Robert E Hill,Wendy A Bickmore,Laura A Lettice
View all publications
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