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AB51506

Anti-SCML2 antibody [SCMAD14A]

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(1 Publication)

Mouse Monoclonal SCML2 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Sex comb on midleg-like protein 2.

View Alternative Names

Sex comb on midleg-like protein 2, SCML2

1 Images
Western blot - Anti-SCML2 antibody [SCMAD14A] (AB51506)
  • WB

Unknown

Western blot - Anti-SCML2 antibody [SCMAD14A] (AB51506)

Same results obtained using NIH3T3 whole cell lysate, and F2408 whole cell lysate.

All lanes:

Western blot - Anti-SCML2 antibody [SCMAD14A] (ab51506) at 1/200 dilution

All lanes:

HeLa whole cell lysate at 25 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 77 kDa

Observed band size: 100 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SCMAD14A

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human Sex comb on midleg-like protein 2. The exact immunogen used to generate this antibody is proprietary information.

Q9UQR0

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab51506 purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1136% Disodium hydrogenorthophosphate, 0.0225% Potassium chloride, 0.0204% Potassium phosphate monobasic
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCML2 also known as Sex Comb on Midleg-Like Protein 2 is a Polycomb group protein involved in transcriptional regulation. It is part of a larger family of proteins characterized by a SPM domain and SCML2 itself has a molecular mass of approximately 100 kDa. SCML2 is expressed in various tissues with significant levels found in testis and brain tissue. Its presence contributes to the maintenance of transcriptional repression of specific genes maintaining proper cellular function.
Biological function summary

SCML2 interacts with other proteins within the Polycomb repressive complex helping regulate gene expression during development and differentiation. SCML2 binds to chromatin and represses genes involved in cell cycle regulation and developmental processes. By modulating these genes it plays an important role in maintaining the silenced state of target genes which is necessary for proper cell function and development.

Pathways

SCML2 participates in the Polycomb group pathway which is important for maintaining gene repression across generations of cells. This protein interacts with other Polycomb proteins like PRC1 and PRC2 which are essential for the propagation of repressive histone marks. These interactions lead to the compacting of chromatin influencing genes concerned with growth and proliferation.

SCML2 dysfunction is linked to certain cancers including testicular cancer due to its role in regulation of gene expression. Disrupted SCML2 may affect the pathways related to BRCA1 a protein that also contributes to genomic stability and suppresses tumors. Studies have shown that abnormalities in SCML2 can lead to imbalance in gene repression making cells more prone to uncontrolled growth and proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development (By similarity).
See full target information Sex comb on midleg-like protein 2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 47:465-72 PubMed26094604

2015

Epigenetic regulation of embryonic stem cell marker miR302C in human chondrosarcoma as determinant of antiproliferative activity of proline-rich polypeptide 1.

Applications

Unspecified application

Species

Unspecified reactive species

Karina Galoian,Amir Qureshi,Gianluca D'Ippolito,Paul C Schiller,Marco Molinari,Andrea L Johnstone,Shaun P Brothers,Ana C Paz,H T Temple
View all publications
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Product promise

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