Rabbit Polyclonal SCML4 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SCML4 aa 1-250.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SCML4 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N228
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development (By similarity).
Alternative names
Sex comb on midleg-like protein 4, SCML4
Recommended products
Rabbit Polyclonal SCML4 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SCML4 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SCML4 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N228
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SCML4 also known as Sex Comb on Midleg-Like 4 is a protein with a molecular mass of approximately 56 kDa. This protein is part of the Polycomb Group (PcG) proteins which are critical regulators of gene expression through chromatin modification. SCML4 binds to DNA influencing gene repression by recruiting other proteins to histones. Expression of SCML4 occurs in a wide range of tissues with noticeable levels in reproductive organs as well as in the central nervous system.
- Biological function summary
SCML4 functions by participating in the repression of gene expression as a member of the Polycomb Repressive Complex (PRC). Within this context SCML4 may interact with other PcG proteins to maintain the repressive histone marks that lead to the compact chromatin state. This activity is significant for regulating genes involved in development including those responsible for cell differentiation and proliferation.
- Pathways
The repression role SCML4 plays is integral to the PRC2 and PRC1 pathways. These pathways help control gene silencing by modifying chromatin structure influencing cell fate decisions. SCML4 may work with proteins such as EZH2 and BMI1 which are also components of these pathways to ensure that genes necessary for development and cell cycle control remain inactive when not needed.
- Associated diseases and disorders
Altered SCML4 function relates to cancer development particularly in prostate and ovarian cancers. Abnormal SCML4 expression or dysfunction can lead to the improper regulation of gene expression resulting in unregulated cell growth and tumorigenesis. Connection to cancer-related proteins like EZH2 highlights SCML4's role in maintaining cellular homeostasis and spotlight its potential as a target for therapeutic intervention in oncology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCML4 antibody (ab154011)
Immunohistochemical analysis of paraffin embedded BT483 xenograft tissue labeling SCML4 with ab154011 at 1/750 dilution.
Western blot - Anti-SCML4 antibody (ab154011)
12% SDS PAGE
All lanes: Western blot - Anti-SCML4 antibody (ab154011) at 1/1000 dilution
All lanes: SK-N-SH whole cell lysate at 30 µg
Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCML4 antibody (ab154011)
Immunohistochemical analysis of paraffin embedded C2C12 xenograft tissue, labeling SCML4 with ab154011 at 1/750 dilution.
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