Anti-SCN11A antibody [EPR28603-66] - BSA and Azide free

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SCN11A antibody [EPR28603-66] - BSA and Azide free (AB316325)

This data was developed using ab316324, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung (fresh frozen) tissue labeling SCN11A with ab316324 at 1/200 (2.455 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Negative control : confocal image showing no staining on mouse lung. The nuclear counterstain was DAPI (Blue). The section was incubated with ab316324 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SCN11A antibody [EPR28603-66] - BSA and Azide free (AB316325)

This data was developed using ab316324, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh frozen) tissue labeling SCN11A with ab316324 at 1/200 (2.455 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Negative control : confocal image showing no staining on mouse liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab316324 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SCN11A antibody [EPR28603-66] - BSA and Azide free (AB316325)

This data was developed using ab316324, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse dorsal root ganglion (fresh frozen) tissue labeling SCN11A with ab316324 at 1/200 (2.455 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Panel A : merged staining of anti-SCN11A (ab316324, green) and anti-NeuN (ab190565, magenta) on mouse dorsal root ganglia.
Panel B : anti-SCN11A stained on mouse dorsal root ganglia.
Panel C : anti-NeuN stained in neurons of mouse dorsal root ganglia.
The section was incubated in two rounds of staining : in the order of ab316324 and ab190565 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

• WB

Supplier Data

Western blot - Anti-SCN11A antibody [EPR28603-66] - BSA and Azide free (AB316325)

This data was developed using ab316324, the same antibody clone in a different buffer formulation.

Negative control : brain, liver(PMID : 11972962, 30395542)

The lower band around 100 kDa could be non-specific band(PMID : 11739573)

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-SCN11A antibody [EPR28603-66] (<a href='/en-us/products/primary-antibodies/scn11a-antibody-epr28603-66-ab316324'>ab316324</a>) at 1/1000 dilution

Lane 1:

Mouse dorsal galion tissue lyaste at 30 µg with 5% NFDM/TBST

Lane 2:

Mouse brain tissue lyaste at 30 µg with 5% NFDM/TBST

Lane 3:

Mouse liver tissue lyaste at 30 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 70 kDa,124 kDa

false

Exposure time: 70s

• WB

Supplier Data

Western blot - Anti-SCN11A antibody [EPR28603-66] - BSA and Azide free (AB316325)

This data was developed using ab316324, the same antibody clone in a different buffer formulation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-SCN11A antibody [EPR28603-66] (<a href='/en-us/products/primary-antibodies/scn11a-antibody-epr28603-66-ab316324'>ab316324</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 10 µg with 5% NFDM/TBST

Lane 2:

293T (human embryonic kidney epithelial cell) cells transfected with human SCN11A expression vector containi a myc-His-tag®, whole cell lysate at 10 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 204 kDa,124 kDa

false

Exposure time: 3s