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AB24820

Anti-Scn1a antibody

3

(7 Reviews)

|

(11 Publications)

Rabbit Polyclonal SCN1A antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 11 publications.

View Alternative Names

NAC1, SCN1, SCN1A, Sodium channel protein type 1 subunit alpha, Sodium channel protein brain I subunit alpha, Sodium channel protein type I subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody (AB24820)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody (AB24820)

Staining of human cerebellum with anti-sodium channel antibody. The tissue was boiled in 10mM citrate buffer, pH 6.0 for 10 mins followed by cooling at room temparture for 20 mins. The working dilution for the antibody is 1 : 200 for 30 min at room temparture.

Immunocytochemistry/ Immunofluorescence - Anti-Scn1a antibody (AB24820)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Scn1a antibody (AB24820)

ICC/IF image of ab24820 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24820, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Ab with sodium azide is stable for 24 months when stored at 2-8 °C.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Scn1a gene encodes the Nav1.1 protein a voltage-gated sodium channel alpha subunit. This protein plays an important role in the initiation and propagation of action potentials in neurons. Nav1.1 has an approximate mass of 260 kDa and is primarily expressed in the central nervous system especially in interneurons. These channels are pivotal for maintaining normal neuronal excitability and their regulation underpins nerve impulse transmission.
Biological function summary

Nav1.1 function influences the balance of excitatory and inhibitory signals in the brain. It forms part of the sodium channel complex working alongside beta subunits to modulate gating properties and channel stability. Dysfunction in Nav1.1 affects neuronal excitability leading to altered synaptic transmission which can impact various neural processes and result in neurophysiological abnormalities.

Pathways

Nav1.1 has an integral role in excitatory signal pathways and is key to the propagation of action potentials in neurons. It interacts closely with proteins like Nav1.2 and Nav1.6 which are also sodium channels contributing to electric signal transmission in the nervous system. Nav1.1 participates in neuronal communication pathways which impact processes like synaptic plasticity and overall brain function.

Alterations in Nav1.1 activity are strongly associated with epilepsy specifically Dravet syndrome. Mutations in SCN1A can lead to defective sodium channel function increasing susceptibility to seizures. Nav1.1 is also linked to other proteins like GABA receptors as impaired inhibitory signaling is involved in the pathology of epilepsy. Furthermore potential associations exist with migraine disorders given the protein's influence on neuronal excitability and signal transmission.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pore-forming subunit of Nav1.1, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na(+) ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (PubMed : 14672992). By regulating the excitability of neurons, ensures that they respond appropriately to synaptic inputs, maintaining the balance between excitation and inhibition in brain neural circuits (By similarity). Nav1.1 plays a role in controlling the excitability and action potential propagation from somatosensory neurons, thereby contributing to the sensory perception of mechanically-induced pain (By similarity).
See full target information SCN1A
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Small methods 6:e2200402 PubMed35595684

2022

Direct Visualization and Identification of Membrane Voltage-Gated Sodium Channels from Human iPSC-Derived Neurons by Multiple Imaging and Light Enhanced Spectroscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Manola Moretti,Tania Limongi,Claudia Testi,Edoardo Milanetti,Maria Teresa De Angelis,Elvira I Parrotta,Stefania Scalise,Gianluca Santamaria,Marco Allione,Sergei Lopatin,Bruno Torre,Peng Zhang,Monica Marini,Gerardo Perozziello,Patrizio Candeloro,Candido Fabrizio Pirri,Giancarlo Ruocco,Giovanni Cuda,Enzo Di Fabrizio

Biomedicines 10: PubMed35625812

2022

Human iPSC Modeling of Genetic Febrile Seizure Reveals Aberrant Molecular and Physiological Features Underlying an Impaired Neuronal Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Stefania Scalise,Clara Zannino,Valeria Lucchino,Michela Lo Conte,Luana Scaramuzzino,Pierangelo Cifelli,Tiziano D'Andrea,Katiuscia Martinello,Sergio Fucile,Eleonora Palma,Antonio Gambardella,Gabriele Ruffolo,Giovanni Cuda,Elvira Immacolata Parrotta

Science advances 7: PubMed33741588

2021

Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Okada,Daisuke Kato,Yuki Nomura,Norihiko Obata,Xiangyu Quan,Akihito Morinaga,Hajime Yano,Zhongtian Guo,Yuki Aoyama,Yoshihisa Tachibana,Andrew J Moorhouse,Osamu Matoba,Tetsuya Takiguchi,Satoshi Mizobuchi,Hiroaki Wake

Frontiers in pharmacology 11:136 PubMed32184723

2020

The Glucagon-Like Peptide-1 Analogue Liraglutide Reduces Seizures Susceptibility, Cognition Dysfunction and Neuronal Apoptosis in a Mouse Model of Dravet Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Shenhai Liu,Zhe Jin,Yiling Zhang,ShiKuo Rong,Wenxin He,Kuisheng Sun,Din Wan,Junming Huo,Lifei Xiao,Xinxiao Li,Na Ding,Feng Wang,Tao Sun

Neuroscience 418:231-243 PubMed31473280

2019

Voltage-Gated Sodium Channels Are Involved in Cognitive Impairments in Parkinson's Disease- like Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyi Wang,Yuying Lin,Weitang Liu,Ping Kuang,Wenwen Lao,Yonghua Ji,Hongyan Zhu

PloS one 13:e0196223 PubMed29715265

2018

Mice lacking the transcriptional regulator Bhlhe40 have enhanced neuronal excitability and impaired synaptic plasticity in the hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly A Hamilton,Yue Wang,Sophia M Raefsky,Sean Berkowitz,Ryan Spangler,Caitlin N Suire,Simonetta Camandola,Robert H Lipsky,Mark P Mattson

Biochimica et biophysica acta. Molecular basis of 1863:1492-1499 PubMed28433711

2017

MDH2 is an RNA binding protein involved in downregulation of sodium channel Scn1a expression under seizure condition.

Applications

WB

Species

Unspecified reactive species

Yong-Hong Chen,Shu-Jing Liu,Mei-Mei Gao,Tao Zeng,Guo-Wang Lin,Na-Na Tan,Hui-Ling Tang,Ping Lu,Tao Su,Wei-Wen Sun,Long-Chang Xie,Yong-Hong Yi,Yue-Sheng Long

Nature protocols 10:1543-55 PubMed26379228

2015

MicroRNA-based conversion of human fibroblasts into striatal medium spiny neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle Richner,Matheus B Victor,Yangjian Liu,Daniel Abernathy,Andrew S Yoo

Circulation. Arrhythmia and electrophysiology 8:912-20 PubMed26067667

2015

Prolongation of action potential duration and QT interval during epilepsy linked to increased contribution of neuronal sodium channels to cardiac late Na+ current: potential mechanism for sudden death in epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Biet,Nathalie Morin,Melissa Lessard-Beaudoin,Rona K Graham,Sandra Duss,Jonathan Gagné,Nathalie T Sanon,Lionel Carmant,Robert Dumaine

PloS one 10:e0125898 PubMed25965391

2015

Pharmacological characterization of an antisense knockdown zebrafish model of Dravet syndrome: inhibition of epileptic seizures by the serotonin agonist fenfluramine.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Zhang,Angéla Kecskés,Daniëlle Copmans,Mélanie Langlois,Alexander D Crawford,Berten Ceulemans,Lieven Lagae,Peter A M de Witte,Camila V Esguerra
View all publications
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