Rabbit Polyclonal SCN1A antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 9 publications.
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- Biomedicines 10:2022Human iPSC Modeling of Genetic Febrile Seizure Reveals Aberrant Molecular and Physiological Features Underlying an Impaired Neuronal Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefania Scalise,Clara Zannino,Valeria Lucchino,Michela Lo Conte,Luana Scaramuzzino,Pierangelo Cifelli,Tiziano D'Andrea,Katiuscia Martinello,Sergio Fucile,Eleonora Palma,Antonio Gambardella,Gabriele Ruffolo,Giovanni Cuda,Elvira Immacolata ParrottaPubMed 35625812
- Science advances 7:2021Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakuya Okada,Daisuke Kato,Yuki Nomura,Norihiko Obata,Xiangyu Quan,Akihito Morinaga,Hajime Yano,Zhongtian Guo,Yuki Aoyama,Yoshihisa Tachibana,Andrew J Moorhouse,Osamu Matoba,Tetsuya Takiguchi,Satoshi Mizobuchi,Hiroaki WakePubMed 33741588
- Neuroscience 418:231-2432019Voltage-Gated Sodium Channels Are Involved in Cognitive Impairments in Parkinson's Disease- like Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiyi Wang et. al.PubMed 31473280
- PloS one 13:e01962232018Mice lacking the transcriptional regulator Bhlhe40 have enhanced neuronal excitability and impaired synaptic plasticity in the hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKelly A Hamilton et. al.PubMed 29715265
- Biochimica et biophysica acta. Molecular basis of 1863:1492-14992017MDH2 is an RNA binding protein involved in downregulation of sodium channel Scn1a expression under seizure condition.
- Nature protocols 10:1543-552015MicroRNA-based conversion of human fibroblasts into striatal medium spiny neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichelle Richner,Matheus B Victor,Yangjian Liu,Daniel Abernathy,Andrew S YooPubMed 26379228
- Circulation. Arrhythmia and electrophysiology 8:912-202015Prolongation of action potential duration and QT interval during epilepsy linked to increased contribution of neuronal sodium channels to cardiac late Na+ current: potential mechanism for sudden death in epilepsy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael Biet et. al.PubMed 26067667
- PloS one 10:e01258982015Pharmacological characterization of an antisense knockdown zebrafish model of Dravet syndrome: inhibition of epileptic seizures by the serotonin agonist fenfluramine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifan Zhang et. al.PubMed 25965391
- Epilepsia 53:e111-42012Different degrees of loss of function between GEFS+ and SMEI Nav 1.1 missense mutants at the same residue induced by rescuable folding defects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshihiro Sugiura,Ikuo Ogiwara,Akihiko Hoshi,Kazuhiro Yamakawa,Yoshikazu UgawaPubMed 22525008
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Predicted |
| Rat | Expected | Tested |
| Rabbit | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Zebrafish | Dilution info - | Notes - |
Associated Products
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Target data
Function
Pore-forming subunit of Nav1.1, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na(+) ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (PubMed:14672992). By regulating the excitability of neurons, ensures that they respond appropriately to synaptic inputs, maintaining the balance between excitation and inhibition in brain neural circuits (By similarity). Nav1.1 plays a role in controlling the excitability and action potential propagation from somatosensory neurons, thereby contributing to the sensory perception of mechanically-induced pain (By similarity).
Alternative names
NAC1, SCN1, SCN1A, Sodium channel protein type 1 subunit alpha, Sodium channel protein brain I subunit alpha, Sodium channel protein type I subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.1
Recommended products
Rabbit Polyclonal SCN1A antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 9 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Ab with sodium azide is stable for 24 months when stored at 2-8 °C.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Scn1a gene encodes the Nav1.1 protein a voltage-gated sodium channel alpha subunit. This protein plays an important role in the initiation and propagation of action potentials in neurons. Nav1.1 has an approximate mass of 260 kDa and is primarily expressed in the central nervous system especially in interneurons. These channels are pivotal for maintaining normal neuronal excitability and their regulation underpins nerve impulse transmission.
- Biological function summary
Nav1.1 function influences the balance of excitatory and inhibitory signals in the brain. It forms part of the sodium channel complex working alongside beta subunits to modulate gating properties and channel stability. Dysfunction in Nav1.1 affects neuronal excitability leading to altered synaptic transmission which can impact various neural processes and result in neurophysiological abnormalities.
- Pathways
Nav1.1 has an integral role in excitatory signal pathways and is key to the propagation of action potentials in neurons. It interacts closely with proteins like Nav1.2 and Nav1.6 which are also sodium channels contributing to electric signal transmission in the nervous system. Nav1.1 participates in neuronal communication pathways which impact processes like synaptic plasticity and overall brain function.
- Associated diseases and disorders
Alterations in Nav1.1 activity are strongly associated with epilepsy specifically Dravet syndrome. Mutations in SCN1A can lead to defective sodium channel function increasing susceptibility to seizures. Nav1.1 is also linked to other proteins like GABA receptors as impaired inhibitory signaling is involved in the pathology of epilepsy. Furthermore potential associations exist with migraine disorders given the protein's influence on neuronal excitability and signal transmission.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody (ab24820)
Staining of human cerebellum with anti-sodium channel antibody. The tissue was boiled in 10mM citrate buffer, pH 6.0 for 10 mins followed by cooling at room temparture for 20 mins. The working dilution for the antibody is 1:200 for 30 min at room temparture.
Immunocytochemistry/ Immunofluorescence - Anti-Scn1a antibody (ab24820)
ICC/IF image of ab24820 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24820, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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