Rabbit Polyclonal SCN2A antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SCN2A.
Images
Publications
- Cell reports 36:1094832021Paradoxical hyperexcitability from Na1.2 sodium channel loss in neocortical pyramidal cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPerry W E Spratt,Ryan P D Alexander,Roy Ben-Shalom,Atehsa Sahagun,Henry Kyoung,Caroline M Keeshen,Stephan J Sanders,Kevin J BenderPubMed 34348157
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: Whole serum
- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human SCN2A. The exact immunogen used to generate this antibody is proprietary information. Database link Q99250
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:1325650, PubMed:17021166, PubMed:28256214, PubMed:29844171). Implicated in the regulation of hippocampal replay occurring within sharp wave ripples (SPW-R) important for memory (By similarity).
Alternative names
Sodium channel protein type 2 subunit alpha, HBSC II, Sodium channel protein brain II subunit alpha, Sodium channel protein type II subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.2, HBA, SCN2A, SCN2A2, NAC2, SCN2A1
Recommended products
Rabbit Polyclonal SCN2A antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SCN2A.
Alternative names
Sodium channel protein type 2 subunit alpha, HBSC II, Sodium channel protein brain II subunit alpha, Sodium channel protein type II subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.2, HBA, SCN2A, SCN2A2, NAC2, SCN2A1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: Whole serum
- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human SCN2A. The exact immunogen used to generate this antibody is proprietary information. Database link Q99250
- Purity
Whole antiserum
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
The SCN2A gene encodes for the Nav1.2 protein a voltage-gated sodium channel alpha subunit with a molecular weight of approximately 260 kDa. This protein acts mechanically by allowing sodium ions to flow into neurons upon depolarization making it fundamental for transmitting electrical signals. Nav1.2 is mainly expressed in the central nervous system especially in the brain regions involved in cognitive functions. Besides Nav1.2 SCI 2A is a popular alternate name for this protein.
- Biological function summary
The Nav1.2 channel contributes to the generation and propagation of action potentials in neurons. It is part of a larger sodium channel complex that regulates neuronal excitability and synaptic transmission. This channel plays an essential role in maintaining the electrical activity that supports processes like learning and memory. Proper function of Nav1.2 ensures precise neuronal communication which is necessary for normal cognitive and neurological activities.
- Pathways
The Nav1.2 channel integrates into the action potential pathway where it participates in the initiation and transmission of nerve impulses. It also intersects with synaptic transmission pathways working alongside proteins like Nav1.1 and Nav1.6 to ensure proper synaptic function. These interactions facilitate an effective communication system necessary for maintaining neural network stability.
- Associated diseases and disorders
Altered Nav1.2 function associates with epilepsy particularly early-onset epileptic encephalopathies. The gene mutations can disrupt normal sodium channel activity leading to increased neuronal excitability and seizure susceptibility. Furthermore variants in SCN2A link to autism spectrum disorders implicating sodium channel deficiencies in abnormal neurodevelopment. The protein interacts with auxiliary proteins like Navβ1 which influences the severity and presentation of these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-SCN2A antibody (ab65163)
ICC/IF image of ab65163 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65163, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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