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AB65163

Anti-SCN2A antibody

1

(1 Review)

|

(3 Publications)

Rabbit Polyclonal SCN2A antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SCN2A.

View Alternative Names

HBA, NAC2, SCN2A1, SCN2A2, SCN2A, Sodium channel protein type 2 subunit alpha, HBSC II, Sodium channel protein brain II subunit alpha, Sodium channel protein type II subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.2

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-SCN2A antibody (AB65163)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SCN2A antibody (AB65163)

ICC/IF image of ab65163 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65163, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human SCN2A. The exact immunogen used to generate this antibody is proprietary information.

Q99250

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Primary Antibodies

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Primary Antibodies

AB8227

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SCN2A gene encodes for the Nav1.2 protein a voltage-gated sodium channel alpha subunit with a molecular weight of approximately 260 kDa. This protein acts mechanically by allowing sodium ions to flow into neurons upon depolarization making it fundamental for transmitting electrical signals. Nav1.2 is mainly expressed in the central nervous system especially in the brain regions involved in cognitive functions. Besides Nav1.2 SCI 2A is a popular alternate name for this protein.
Biological function summary

The Nav1.2 channel contributes to the generation and propagation of action potentials in neurons. It is part of a larger sodium channel complex that regulates neuronal excitability and synaptic transmission. This channel plays an essential role in maintaining the electrical activity that supports processes like learning and memory. Proper function of Nav1.2 ensures precise neuronal communication which is necessary for normal cognitive and neurological activities.

Pathways

The Nav1.2 channel integrates into the action potential pathway where it participates in the initiation and transmission of nerve impulses. It also intersects with synaptic transmission pathways working alongside proteins like Nav1.1 and Nav1.6 to ensure proper synaptic function. These interactions facilitate an effective communication system necessary for maintaining neural network stability.

Altered Nav1.2 function associates with epilepsy particularly early-onset epileptic encephalopathies. The gene mutations can disrupt normal sodium channel activity leading to increased neuronal excitability and seizure susceptibility. Furthermore variants in SCN2A link to autism spectrum disorders implicating sodium channel deficiencies in abnormal neurodevelopment. The protein interacts with auxiliary proteins like Navβ1 which influences the severity and presentation of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed : 1325650, PubMed : 17021166, PubMed : 28256214, PubMed : 29844171). Implicated in the regulation of hippocampal replay occurring within sharp wave ripples (SPW-R) important for memory (By similarity).
See full target information SCN2A
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Neuron 112:1133-1149.e6 PubMed38290518

2024

Physical and functional convergence of the autism risk genes Scn2a and Ank2 in neocortical pyramidal cell dendrites.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew D Nelson,Amanda M Catalfio,Julie P Gupta,Lia Min,René N Caballero-Florán,Kendall P Dean,Carina C Elvira,Kimberly D Derderian,Henry Kyoung,Atehsa Sahagun,Stephan J Sanders,Kevin J Bender,Paul M Jenkins

Cell reports 36:109483 PubMed34348157

2021

Paradoxical hyperexcitability from Na1.2 sodium channel loss in neocortical pyramidal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Perry W E Spratt,Ryan P D Alexander,Roy Ben-Shalom,Atehsa Sahagun,Henry Kyoung,Caroline M Keeshen,Stephan J Sanders,Kevin J Bender

Journal of cell science 124:1136-44 PubMed21385842

2011

Regulation of mouse embryonic stem cell self-renewal by a Yes-YAP-TEAD2 signaling pathway downstream of LIF.

Applications

Unspecified application

Species

Unspecified reactive species

Christoffer Tamm,Nathalie Böwer,Cecilia Annerén
View all publications
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Product promise

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