Rabbit Polyclonal SCN3A antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SCN3A aa 50-150.
Images
Publications
Filter by
- Cell genomics 3:1002502023Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGreta Pintacuda,Yu-Han H Hsu,Kalliopi Tsafou,Ka Wan Li,Jacqueline M Martín,Jackson Riseman,Julia C Biagini,Joshua K T Ching,Daya Mena,Miguel A Gonzalez-Lozano,Shawn B Egri,Jake Jaffe,August B Smit,Nadine Fornelos,Kevin C Eggan,Kasper LagePubMed 36950384
- Neuroscience 418:231-2432019Voltage-Gated Sodium Channels Are Involved in Cognitive Impairments in Parkinson's Disease- like Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiyi Wang et. al.PubMed 31473280
- Neuroscience letters 687:146-1522018Re-expression of voltage-gated sodium channel subtype Nav1.3 in the substantia nigra after dopamine depletion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiyi Wang et. al.PubMed 30267849
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: Whole serum
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SCN3A aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NY46
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Pore-forming subunit of Nav1.3, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na+ ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (PubMed:24157691, PubMed:28235671, PubMed:29466837, PubMed:35277491). In some secretory cell types, it also participates in cell excitability through membrane depolarization and regulates cells responsiveness to stimuli triggering secretion. For instance, it controls the release of serotonin/5-hydroxytryptamine by enterochromaffin cells and is required for both glucagon- and glucose-induced insulin secretion in pancreatic endocrine cells (By similarity).
Alternative names
KIAA1356, NAC3, SCN3A, Sodium channel protein type 3 subunit alpha, Sodium channel protein brain III subunit alpha, Sodium channel protein type III subunit alpha, Voltage-gated sodium channel subtype III, Voltage-gated sodium channel subunit alpha Nav1.3
Recommended products
Rabbit Polyclonal SCN3A antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SCN3A aa 50-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: Whole serum
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SCN3A aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NY46
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SCN3A also known as sodium channel voltage-gated type III alpha subunit or NaV1.3 plays a mechanical role in the conduction of electrical signals in excitable cells. It forms part of the voltage-gated sodium channels that are critical in the generation and propagation of action potentials. SCN3A has a molecular mass of approximately 260 kDa and is expressed broadly in the central nervous system particularly in neurons.
- Biological function summary
The voltage-gated sodium channel SCN3A contributes to rapid depolarization in neuronal action potentials. It is an important component of a larger sodium channel complex which includes beta subunits that regulate the kinetic properties and localization of these channels. This enables proper neuronal signaling and communication affecting processes such as neurotransmission and response to stimuli.
- Pathways
SCN3A influences the regulation of neuronal excitability and synaptic activity. It operates within the action potential pathway and has connections to the calcium signaling pathway which impacts numerous cellular processes. SCN3A functions alongside proteins such as SCN1A and SCN2A within these pathways contributing to the integrated organization of electrical signaling in neurons.
- Associated diseases and disorders
Mutations or dysregulations in SCN3A relate to epilepsy and neurodevelopmental disorders. SCN3A's interaction with SCN1A is significant as alterations in sodium channel function can lead to imbalance in neuronal excitability and seizures. These relationships highlight the importance of SCN3A in maintaining normal neural function and its potential as a therapeutic target in related neurologic conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Immunocytochemistry/ Immunofluorescence - Anti-SCN3A antibody (ab65164)
ICC/IF image of ab65164 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65164, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com