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Rabbit Recombinant Monoclonal SCN4A antibody. Suitable for IHC-Fr and reacts with Rat, Mouse samples.

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Images

Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (AB306585), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (AB306585), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (AB306585), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (AB306585), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (AB306585), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-FrIHC-PWB
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

-

Species
Mouse
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Pore-forming subunit of Nav1.4, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na+ ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (PubMed:11834499). Highly expressed in skeletal muscles, Nav1.4 generates the action potential crucial for muscle contraction (PubMed:18317596, PubMed:21881211).

Alternative names

Sodium channel protein type 4 subunit alpha, Sodium channel protein skeletal muscle subunit alpha, Sodium channel protein type IV subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.4, Scn4a

Recommended products

Rabbit Recombinant Monoclonal SCN4A antibody. Suitable for IHC-Fr and reacts with Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24138-282
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The SCN4A gene encodes the Nav1.4 protein also known as the sodium channel voltage-gated type IV alpha subunit. Nav1.4 is a voltage-gated sodium channel with a molecular weight around 250 kDa. It mainly facilitates the rapid influx of sodium ions into skeletal muscle cells which is important for the initiation and conduction of action potentials. Expression of Nav1.4 is strong in skeletal muscle tissues playing an important role in muscle excitability and contraction. It serves as a pivotal point in the regulation of skeletal muscle function.

Biological function summary

Nav1.4 is essential in generating and propagating action potentials necessary for muscle contraction. This protein functions as part of a complex that includes beta subunits that help modulate its properties. The precise gating and ion permeability of Nav1.4 are important for proper muscle responsiveness. Nav1.4's activity ensures proper muscle tone and responsiveness to neurological inputs impacting motor function and muscle reflexes directly.

Pathways

Nav1.4 is integral to pathways involved in action potential propagation and muscle contraction. It forms part of the larger excitable membrane network that includes calcium channels such as Cav1.1 and proteins like ryanodine receptors (RYRs) which participate in muscle excitation-contraction coupling. Nav1.4 has a synergistic relationship with these proteins enabling the effective transmission of electrical signals that trigger muscle contraction in response to stimuli.

Associated diseases and disorders

Alterations in SCN4A can lead to conditions like Hyperkalemic Periodic Paralysis and Paramyotonia Congenita. Mutations affecting Nav1.4 disrupt normal muscle excitability leading to symptoms such as muscle weakness and temporary paralysis. There are associations between these disorders and other ion channels like Kir2.1 which can compound effects on membrane excitability. Understanding the role of Nav1.4 in these diseases aids in developing therapeutic strategies for muscle channelopathies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (ab306585), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (ab306585)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling SCN4A with ab306585 at 1/500 (1.02 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

    Negative control: confocal image showing no staining on rat liver (PMID: 24309898).

    The section was incubated with ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (ab306585), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (ab306585)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling SCN4A with ab306585 at 1/500 (1.02 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

    Negative control: confocal image showing no staining on mouse cerebrum (PMID:22081212).

    The section was incubated with ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (ab306585), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (ab306585)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling SCN4A with ab306585 at 1/500 (1.02 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

    Negative control: confocal image showing no staining on rat cerebrum (PMID:22081212).

    The section was incubated with ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (ab306585), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (ab306585)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labeling SCN4A with ab306585 at 1/500 (1.02 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on rat skeletal muscle. The section was incubated with ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (ab306585), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (ab306585)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling SCN4A with ab306585 at 1/500 (1.02 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on mouse skeletal muscle. The section was incubated with ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (ab306585), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] (ab306585)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling SCN4A with ab306585 at 1/500 (1.02 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

    Negative control: confocal image showing no staining on mouse liver (PMID: 24309898).

    The section was incubated with ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

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Product protocols

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