Rabbit Recombinant Monoclonal SCN4A antibody. Carrier free. Suitable for IHC-Fr and reacts with Rat, Mouse samples.
Images
![Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586), expandable thumbnail](https://content.abcam.com/products/images/scn4a-antibody-epr24138-282-bsa-and-azide-free-ab306586--immunohistochemistry-frozen-sections-img404946.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)")
![Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586), expandable thumbnail](https://content.abcam.com/products/images/scn4a-antibody-epr24138-282-bsa-and-azide-free-ab306586--immunohistochemistry-frozen-sections-img404942.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)")
![Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586), expandable thumbnail](https://content.abcam.com/products/images/scn4a-antibody-epr24138-282-bsa-and-azide-free-ab306586--immunohistochemistry-frozen-sections-img404945.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)")
![Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586), expandable thumbnail](https://content.abcam.com/products/images/scn4a-antibody-epr24138-282-bsa-and-azide-free-ab306586--immunohistochemistry-frozen-sections-img404947.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)")
![Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586), expandable thumbnail](https://content.abcam.com/products/images/scn4a-antibody-epr24138-282-bsa-and-azide-free-ab306586--immunohistochemistry-frozen-sections-img404944.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-Fr | IHC-P | WB | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Pore-forming subunit of Nav1.4, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na+ ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (PubMed:11834499). Highly expressed in skeletal muscles, Nav1.4 generates the action potential crucial for muscle contraction (PubMed:18317596, PubMed:21881211).
Alternative names
Sodium channel protein type 4 subunit alpha, Sodium channel protein skeletal muscle subunit alpha, Sodium channel protein type IV subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.4, Scn4a
Recommended products
Rabbit Recombinant Monoclonal SCN4A antibody. Carrier free. Suitable for IHC-Fr and reacts with Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24138-282
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SCN4A gene encodes the Nav1.4 protein also known as the sodium channel voltage-gated type IV alpha subunit. Nav1.4 is a voltage-gated sodium channel with a molecular weight around 250 kDa. It mainly facilitates the rapid influx of sodium ions into skeletal muscle cells which is important for the initiation and conduction of action potentials. Expression of Nav1.4 is strong in skeletal muscle tissues playing an important role in muscle excitability and contraction. It serves as a pivotal point in the regulation of skeletal muscle function.
- Biological function summary
Nav1.4 is essential in generating and propagating action potentials necessary for muscle contraction. This protein functions as part of a complex that includes beta subunits that help modulate its properties. The precise gating and ion permeability of Nav1.4 are important for proper muscle responsiveness. Nav1.4's activity ensures proper muscle tone and responsiveness to neurological inputs impacting motor function and muscle reflexes directly.
- Pathways
Nav1.4 is integral to pathways involved in action potential propagation and muscle contraction. It forms part of the larger excitable membrane network that includes calcium channels such as Cav1.1 and proteins like ryanodine receptors (RYRs) which participate in muscle excitation-contraction coupling. Nav1.4 has a synergistic relationship with these proteins enabling the effective transmission of electrical signals that trigger muscle contraction in response to stimuli.
- Associated diseases and disorders
Alterations in SCN4A can lead to conditions like Hyperkalemic Periodic Paralysis and Paramyotonia Congenita. Mutations affecting Nav1.4 disrupt normal muscle excitability leading to symptoms such as muscle weakness and temporary paralysis. There are associations between these disorders and other ion channels like Kir2.1 which can compound effects on membrane excitability. Understanding the role of Nav1.4 in these diseases aids in developing therapeutic strategies for muscle channelopathies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586), expandable thumbnail](https://content.abcam.com/products/images/scn4a-antibody-epr24138-282-bsa-and-azide-free-ab306586--immunohistochemistry-frozen-sections-img404946.jpg "Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586)")
Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586)
This data was developed using Anti-SCN4A antibody [EPR24138-282] ab306585, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling SCN4A with Anti-SCN4A antibody [EPR24138-282] ab306585 at 1/500 (1.02 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Negative control: confocal image showing no staining on rat liver (PMID: 24309898).
The section was incubated with Anti-SCN4A antibody [EPR24138-282] ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.![Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586), expandable thumbnail](https://content.abcam.com/products/images/scn4a-antibody-epr24138-282-bsa-and-azide-free-ab306586--immunohistochemistry-frozen-sections-img404942.jpg "Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586)")
Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586)
This data was developed using Anti-SCN4A antibody [EPR24138-282] ab306585, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling SCN4A with Anti-SCN4A antibody [EPR24138-282] ab306585 at 1/500 (1.02 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Negative control: confocal image showing no staining on mouse cerebrum (PMID:22081212).
The section was incubated with Anti-SCN4A antibody [EPR24138-282] ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.![Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586), expandable thumbnail](https://content.abcam.com/products/images/scn4a-antibody-epr24138-282-bsa-and-azide-free-ab306586--immunohistochemistry-frozen-sections-img404945.jpg "Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586)")
Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586)
This data was developed using Anti-SCN4A antibody [EPR24138-282] ab306585, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling SCN4A with Anti-SCN4A antibody [EPR24138-282] ab306585 at 1/500 (1.02 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Negative control: confocal image showing no staining on rat cerebrum (PMID:22081212).
The section was incubated with Anti-SCN4A antibody [EPR24138-282] ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.![Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586), expandable thumbnail](https://content.abcam.com/products/images/scn4a-antibody-epr24138-282-bsa-and-azide-free-ab306586--immunohistochemistry-frozen-sections-img404947.jpg "Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586)")
Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586)
This data was developed using Anti-SCN4A antibody [EPR24138-282] ab306585, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labeling SCN4A with Anti-SCN4A antibody [EPR24138-282] ab306585 at 1/500 (1.02 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on rat skeletal muscle. The section was incubated with Anti-SCN4A antibody [EPR24138-282] ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.![Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586), expandable thumbnail](https://content.abcam.com/products/images/scn4a-antibody-epr24138-282-bsa-and-azide-free-ab306586--immunohistochemistry-frozen-sections-img404944.jpg "Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586)")
Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586)
This data was developed using Anti-SCN4A antibody [EPR24138-282] ab306585, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling SCN4A with Anti-SCN4A antibody [EPR24138-282] ab306585 at 1/500 (1.02 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on mouse skeletal muscle. The section was incubated with Anti-SCN4A antibody [EPR24138-282] ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.![Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586), expandable thumbnail](https://content.abcam.com/products/images/scn4a-antibody-epr24138-282-bsa-and-azide-free-ab306586--immunohistochemistry-frozen-sections-img404943.jpg "Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586)")
Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586)
This data was developed using Anti-SCN4A antibody [EPR24138-282] ab306585, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling SCN4A with Anti-SCN4A antibody [EPR24138-282] ab306585 at 1/500 (1.02 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Negative control: confocal image showing no staining on mouse liver (PMID: 24309898).
The section was incubated with Anti-SCN4A antibody [EPR24138-282] ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
![Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586), expandable thumbnail](https://content.abcam.com/products/images/scn4a-antibody-epr24138-282-bsa-and-azide-free-ab306586--immunohistochemistry-frozen-sections-img404943.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (ab306586)")
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