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AB306586

Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free

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Rabbit Recombinant Monoclonal SCN4A antibody. Carrier free. Suitable for IHC-Fr and reacts with Rat, Mouse samples.

View Alternative Names

Sodium channel protein type 4 subunit alpha, Sodium channel protein skeletal muscle subunit alpha, Sodium channel protein type IV subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.4, Scn4a

6 Images
Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)

This data was developed using ab306585, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling SCN4A with ab306585 at 1/500 (1.02 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control : confocal image showing no staining on rat liver (PMID : 24309898). The section was incubated with ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)

This data was developed using ab306585, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling SCN4A with ab306585 at 1/500 (1.02 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control : confocal image showing no staining on rat cerebrum (PMID : 22081212). The section was incubated with ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)

This data was developed using ab306585, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling SCN4A with ab306585 at 1/500 (1.02 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control : confocal image showing no staining on mouse liver (PMID : 24309898). The section was incubated with ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)

This data was developed using ab306585, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling SCN4A with ab306585 at 1/500 (1.02 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control : confocal image showing no staining on mouse cerebrum (PMID : 22081212). The section was incubated with ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)

This data was developed using ab306585, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labeling SCN4A with ab306585 at 1/500 (1.02 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on rat skeletal muscle. The section was incubated with ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SCN4A antibody [EPR24138-282] - BSA and Azide free (AB306586)

This data was developed using ab306585, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling SCN4A with ab306585 at 1/500 (1.02 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on mouse skeletal muscle. The section was incubated with ab306585 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24138-282

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SCN4A gene encodes the Nav1.4 protein also known as the sodium channel voltage-gated type IV alpha subunit. Nav1.4 is a voltage-gated sodium channel with a molecular weight around 250 kDa. It mainly facilitates the rapid influx of sodium ions into skeletal muscle cells which is important for the initiation and conduction of action potentials. Expression of Nav1.4 is strong in skeletal muscle tissues playing an important role in muscle excitability and contraction. It serves as a pivotal point in the regulation of skeletal muscle function.
Biological function summary

Nav1.4 is essential in generating and propagating action potentials necessary for muscle contraction. This protein functions as part of a complex that includes beta subunits that help modulate its properties. The precise gating and ion permeability of Nav1.4 are important for proper muscle responsiveness. Nav1.4's activity ensures proper muscle tone and responsiveness to neurological inputs impacting motor function and muscle reflexes directly.

Pathways

Nav1.4 is integral to pathways involved in action potential propagation and muscle contraction. It forms part of the larger excitable membrane network that includes calcium channels such as Cav1.1 and proteins like ryanodine receptors (RYRs) which participate in muscle excitation-contraction coupling. Nav1.4 has a synergistic relationship with these proteins enabling the effective transmission of electrical signals that trigger muscle contraction in response to stimuli.

Alterations in SCN4A can lead to conditions like Hyperkalemic Periodic Paralysis and Paramyotonia Congenita. Mutations affecting Nav1.4 disrupt normal muscle excitability leading to symptoms such as muscle weakness and temporary paralysis. There are associations between these disorders and other ion channels like Kir2.1 which can compound effects on membrane excitability. Understanding the role of Nav1.4 in these diseases aids in developing therapeutic strategies for muscle channelopathies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pore-forming subunit of Nav1.4, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na+ ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (PubMed : 11834499). Highly expressed in skeletal muscles, Nav1.4 generates the action potential crucial for muscle contraction (PubMed : 18317596, PubMed : 21881211).
See full target information SCN4A
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Product promise

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