Anti-SCN4B antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SCN4B antibody (AB80539)

ICC/IF image of ab80539 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80539, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• WB

Unknown

Western blot - Anti-SCN4B antibody (AB80539)

Further characterization on this product is underway. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. SCN4B contains at least three potential glycosylation sites (SwissProt), which may explain its migration at a higher molecular weight than predicted. The primary sequence contains a 30-residue signal sequence at the N-terminus. The band around 20-kDa in human skeletal muscle may be the unglycosylated form of the target protein.

All lanes:

Western blot - Anti-SCN4B antibody (ab80539) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg/mL

Lane 2:

Brain (Rat) Tissue Lysate at 10 µg/mL

Lane 3:

U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate at 10 µg/mL

Lane 4:

Human spinal cord tissue lysate - total protein (<a href='/en-us/products/unavailable/human-spinal-cord-tissue-lysate-total-protein-ab29188'>ab29188</a>) at 10 µg/mL

Lane 5:

Spinal Cord (Rat) Tissue Lysate at 10 µg/mL

Lane 6:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg/mL

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 25 kDa

Observed band size: 20 kDa,37 kDa

true

Exposure time: 8min