Rabbit Monoclonal SCN7A antibody. Suitable for I-ELISA, WB and reacts with Recombinant fragment - Mouse, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
I-ELISA | WB | IHC-Fr | IHC-P | ICC/IF | |
---|---|---|---|---|---|
Mouse | Expected | Tested | Not recommended | Not recommended | Not recommended |
Rat | Expected | Tested | Not recommended | Not recommended | Not recommended |
Recombinant fragment - Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Mouse | Dilution info 250 ng/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Recombinant fragment - Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Recombinant fragment - Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Recombinant fragment - Mouse | Dilution info - | Notes - |
Sodium leak channel functioning as an osmosensor regulating sodium ion levels in various tissues and organs. While most sodium channels are voltage-gated, SCN7A is not and lets sodium flow through membrane along its concentration gradient (PubMed:11027237, PubMed:11992118, PubMed:17408578, PubMed:27252474). In glial cells of the central nervous system, senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake through activation of nearby neurons to maintain appropriate sodium levels in the body (PubMed:11027237, PubMed:17408578, PubMed:27252474). By mediating sodium influx into keratinocytes, also plays a role in skin barrier homeostasis (By similarity).
Nag, Nav2, scl11, Sodium channel protein type 7 subunit alpha, Nax channel, Sodium channel Nav2.3
Rabbit Monoclonal SCN7A antibody. Suitable for I-ELISA, WB and reacts with Recombinant fragment - Mouse, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: kidney(PMID:1379737)
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467) at 1/1000 dilution
Lane 1: Rat heart tissue lysate at 50 µg
Lane 2: Rat kidney tissue lysate at 50 µg
Lane 3: Rat P14 spinal cord tissue lysate at 50 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 230 kDa, 124 kDa
Exposure time: 26s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: kidney(PMID:1379737)
Low expression: brain(PMID:1379737)
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467) at 1/1000 dilution
Lane 1: Mouse spinal cord tissue lysate at 50 µg
Lane 2: Mouse brain tissue lysate at 50 µg
Lane 3: Mouse kidney tissue lysate at 50 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 230 kDa, 124 kDa
Exposure time: 81s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: spleen(PMID:1379737)
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467) at 1/1000 dilution
Lane 1: Mouse dorsal ganglion tissue lysate at 50 µg
Lane 2: Mouse spleen tissue lysate at 50 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 230 kDa, 124 kDa
Exposure time: 15s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: skeletal muscle(PMID:1379737)
To minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467) at 1/1000 dilution
Lane 1: Mouse heart tissue lysate at 50 µg
Lane 2: Mouse skeletal muscle tissue lysate at 50 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 230 kDa, 124 kDa
Exposure time: 70s
Indirect ELISA analysis of ab322467 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Mouse SCN7A, Mouse SCN9A.
Antigen concentration: 1000 ng/ml
This antibody does not cross-react with mouse SCN9A
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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