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Rabbit Monoclonal SCN7A antibody. Suitable for I-ELISA, WB and reacts with Recombinant fragment - Mouse, Mouse, Rat samples.

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Images

Western blot - Anti-SCN7A antibody [EPR27096-569] (AB322467), expandable thumbnail
  • Western blot - Anti-SCN7A antibody [EPR27096-569] (AB322467), expandable thumbnail
  • Western blot - Anti-SCN7A antibody [EPR27096-569] (AB322467), expandable thumbnail
  • Western blot - Anti-SCN7A antibody [EPR27096-569] (AB322467), expandable thumbnail
  • Indirect ELISA - Anti-SCN7A antibody [EPR27096-569] (AB322467), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
I-ELISAWBIHC-FrIHC-PICC/IF
Mouse
Expected
Tested
Not recommended
Not recommended
Not recommended
Rat
Expected
Tested
Not recommended
Not recommended
Not recommended
Recombinant fragment - Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Mouse

Dilution info

250 ng/mL

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Recombinant fragment - Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Recombinant fragment - Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Recombinant fragment - Mouse

Dilution info

-

Notes

-

Target data

Function

Sodium leak channel functioning as an osmosensor regulating sodium ion levels in various tissues and organs. While most sodium channels are voltage-gated, SCN7A is not and lets sodium flow through membrane along its concentration gradient (PubMed:11027237, PubMed:11992118, PubMed:17408578, PubMed:27252474). In glial cells of the central nervous system, senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake through activation of nearby neurons to maintain appropriate sodium levels in the body (PubMed:11027237, PubMed:17408578, PubMed:27252474). By mediating sodium influx into keratinocytes, also plays a role in skin barrier homeostasis (By similarity).

Alternative names

Rabbit Monoclonal SCN7A antibody. Suitable for I-ELISA, WB and reacts with Recombinant fragment - Mouse, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467), expandable thumbnail

    Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: kidney(PMID:1379737)

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467) at 1/1000 dilution

    Lane 1: Rat heart tissue lysate at 50 µg

    Lane 2: Rat kidney tissue lysate at 50 µg

    Lane 3: Rat P14 spinal cord tissue lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 230 kDa, 124 kDa

    Exposure time: 26s

  • Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467), expandable thumbnail

    Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: kidney(PMID:1379737)

    Low expression: brain(PMID:1379737)

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467) at 1/1000 dilution

    Lane 1: Mouse spinal cord tissue lysate at 50 µg

    Lane 2: Mouse brain tissue lysate at 50 µg

    Lane 3: Mouse kidney tissue lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 230 kDa, 124 kDa

    Exposure time: 81s

  • Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467), expandable thumbnail

    Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: spleen(PMID:1379737)

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467) at 1/1000 dilution

    Lane 1: Mouse dorsal ganglion tissue lysate at 50 µg

    Lane 2: Mouse spleen tissue lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 230 kDa, 124 kDa

    Exposure time: 15s

  • Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467), expandable thumbnail

    Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: skeletal muscle(PMID:1379737)

    To minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-SCN7A antibody [EPR27096-569] (ab322467) at 1/1000 dilution

    Lane 1: Mouse heart tissue lysate at 50 µg

    Lane 2: Mouse skeletal muscle tissue lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 230 kDa, 124 kDa

    Exposure time: 70s

  • Indirect ELISA - Anti-SCN7A antibody [EPR27096-569] (ab322467), expandable thumbnail

    Indirect ELISA - Anti-SCN7A antibody [EPR27096-569] (ab322467)

    Indirect ELISA analysis of ab322467 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.

    Antigen: Mouse SCN7A, Mouse SCN9A.

    Antigen concentration: 1000 ng/ml

    This antibody does not cross-react with mouse SCN9A

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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