AB241050

Anti-SCNN1B antibody [7B8] - C-terminal

Name: Anti-SCNN1B antibody [7B8] - C-terminal (ab241050) | Abcam
Brand: Abcam Limited
SKU: AB241050

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(1 Publication)

Mouse Monoclonal SCNN1B antibody. C-terminal. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Rat Scnn1b aa 600 to C-terminus.

View Alternative Names

Epithelial sodium channel subunit beta, Amiloride-sensitive sodium channel subunit beta, Beta-NaCH, Epithelial Na(+) channel subunit beta, Nonvoltage-gated sodium channel 1 subunit beta, SCNEB, Beta-ENaC, Scnn1b

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCNN1B antibody [7B8] - C-terminal (AB241050)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCNN1B antibody [7B8] - C-terminal (AB241050)

Formalin-fixed, paraffin-embedded mouse kidney cortex tissue stained for SCNN1B with ab241050 at 1/150 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-SCNN1B antibody [7B8] - C-terminal (AB241050)

All lanes:

Western blot - Anti-SCNN1B antibody [7B8] - C-terminal (ab241050) at 1/1000 dilution

Lane 1:

Mouse kidney homogenates from a mouse on a low-salt diet at 20 µg

Lane 2:

Mouse kidney homogenates from a mouse on a normal salt diet at 20 µg

Predicted band size: 73 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7B8

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Rat Scnn1b aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P37090

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from TCS.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCNN1B also known as the beta subunit of the epithelial sodium channel (ENaC) is a critical component in sodium ion transport across epithelial cells. SCNN1B has a molecular mass of approximately 92 kDa. It is primarily expressed in epithelial tissues like the kidney lung and colon where it contributes to maintenance of sodium balance and regulation of fluid homeostasis. The protein plays an essential role in controlling sodium absorption which is necessary for various physiological processes.

Biological function summary

SCNN1B is a part of the epithelial sodium channel complex which includes two other subunits: SCNN1A (alpha) and SCNN1G (gamma). Together with these subunits SCNN1B facilitates the selective transport of sodium ions impacting processes such as blood pressure regulation and airway surface liquid homeostasis. The channel formed by these subunits operates under guidance by aldosterone a hormone that influences its assembly and activity.

Pathways

SCNN1B takes part in the Renin-Angiotensin-Aldosterone System (RAAS) and sodium reabsorption pathways. These are vital for maintaining electrolyte balance and blood pressure regulation. SCNN1B interacts closely with proteins such as Nedd4-2 which modulates its activity by influencing channel degradation. This interaction ensures that sodium transport and fluid balance remain tightly regulated.

Abnormalities in SCNN1B function relate to conditions like Liddle syndrome and hypertension. Mutations causing hyperactivity of the channel can lead to excessive sodium reabsorption resulting in these conditions. Furthermore SCNN1B has connections with other proteins like SGK1 which plays a regulatory role in adapting sodium channel activity. Understanding these links is important for targeting these conditions with medical therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis (PubMed : 10409305). ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically (PubMed : 9990092). It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys (PubMed : 9990092). This subunit is not essential for ENaC function in airway surface liquid homeostasis and proper mucus clearance (PubMed : 9990092).

See full target information Scnn1b

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed39657788

2024

Novel BRCA1-PLK1-CIP2A axis orchestrates homologous recombination-mediated DNA repair to maintain chromosome integrity during oocyte meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Crystal Lee,Jeong Su Oh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SCNN1B antibody [7B8] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCNN1B antibody [7B8] - C-terminal (AB241050)

Western blot - Anti-SCNN1B antibody [7B8] - C-terminal (AB241050)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise