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AB79172

Anti-SCNN1B (phospho T615) antibody

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(1 Publication)

Rabbit Polyclonal SCNN1B phospho T615 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Amiloride-sensitive sodium channel subunit beta phospho T615.

View Alternative Names

Amiloride-sensitive sodium channel subunit beta, Beta-NaCH, Epithelial Na(+) channel subunit beta, Nonvoltage-gated sodium channel 1 subunit beta, SCNEB, Beta-ENaC, ENaCB, SCNN1B

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCNN1B (phospho T615) antibody (AB79172)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCNN1B (phospho T615) antibody (AB79172)

Immunohistochemiscal analysis of SCNN1B in paraffin embedded human brain tissue using a 1/50 dilution of ab79172 in the presence (right) or absence (left) of the immunizing phosphopeptide.

Immunocytochemistry/ Immunofluorescence - Anti-SCNN1B (phospho T615) antibody (AB79172)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SCNN1B (phospho T615) antibody (AB79172)

Immunofluorescence analysis of SCNN1B in COS7 cells, using a 1/500 dilution of ab79172, in the presence (right) or absence (left) of the immunizing phosphopeptide.

Western blot - Anti-SCNN1B (phospho T615) antibody (AB79172)
  • WB

Unknown

Western blot - Anti-SCNN1B (phospho T615) antibody (AB79172)

All lanes:

Western blot - Anti-SCNN1B (phospho T615) antibody (ab79172) at 1/500 dilution

Lane 1:

extracts from HeLa cells at 10 µg

Lane 2:

extracts from HeLa cells at 10 µg with immunizing phosphopeptide

Predicted band size: 73 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, African green monkey

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Amiloride-sensitive sodium channel subunit beta phospho T615. The exact immunogen used to generate this antibody is proprietary information.

P51168

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCNN1B also known as the beta subunit of the epithelial sodium channel (ENaC) is a critical component in sodium ion transport across epithelial cells. SCNN1B has a molecular mass of approximately 92 kDa. It is primarily expressed in epithelial tissues like the kidney lung and colon where it contributes to maintenance of sodium balance and regulation of fluid homeostasis. The protein plays an essential role in controlling sodium absorption which is necessary for various physiological processes.
Biological function summary

SCNN1B is a part of the epithelial sodium channel complex which includes two other subunits: SCNN1A (alpha) and SCNN1G (gamma). Together with these subunits SCNN1B facilitates the selective transport of sodium ions impacting processes such as blood pressure regulation and airway surface liquid homeostasis. The channel formed by these subunits operates under guidance by aldosterone a hormone that influences its assembly and activity.

Pathways

SCNN1B takes part in the Renin-Angiotensin-Aldosterone System (RAAS) and sodium reabsorption pathways. These are vital for maintaining electrolyte balance and blood pressure regulation. SCNN1B interacts closely with proteins such as Nedd4-2 which modulates its activity by influencing channel degradation. This interaction ensures that sodium transport and fluid balance remain tightly regulated.

Abnormalities in SCNN1B function relate to conditions like Liddle syndrome and hypertension. Mutations causing hyperactivity of the channel can lead to excessive sodium reabsorption resulting in these conditions. Furthermore SCNN1B has connections with other proteins like SGK1 which plays a regulatory role in adapting sodium channel activity. Understanding these links is important for targeting these conditions with medical therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
See full target information Amiloride-sensitive sodium channel subunit beta phospho T615
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 2: PubMed25347857

2014

Nerve growth factor reduces amiloride-sensitive Na+ transport in human airway epithelial cells.

Applications

WB

Species

Human

Michael J Shimko,Eric J Zaccone,Janet A Thompson,Diane Schwegler-Berry,Michael L Kashon,Jeffrey S Fedan
View all publications
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