Rabbit Recombinant Monoclonal SCP1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Images
![Western blot - Anti-SCP1 antibody [EP7918] (AB175191), expandable thumbnail](https://content.abcam.com/products/images/scp1-antibody-ep7918-ab175191--western-blot-img419938.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SCP1 antibody [EP7918] (AB175191)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (AB175191), expandable thumbnail](https://content.abcam.com/products/images/scp1-antibody-ep7918-ab175191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img419941.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (AB175191)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (AB175191), expandable thumbnail](https://content.abcam.com/products/images/scp1-antibody-ep7918-ab175191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img419940.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (AB175191)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (AB175191), expandable thumbnail](https://content.abcam.com/products/images/scp1-antibody-ep7918-ab175191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img419939.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (AB175191)")
Publications
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- iScience 25:1055522022Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiqian Min,Huan Xin,Xiaowen Liu,Jingyu Wan,Ziling Fan,Xinxu Rao,Jiahui Fan,Lifang Yang,Dan LiPubMed 36444304
- Molecular reproduction and development 86:252-2642019Sohlh1 is required for synaptonemal complex formation by transcriptionally regulating meiotic genes during spermatogenesis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Li,Wanjing Qi,Gongqing Liu,Bing Du,Qi Sun,Xue Zhang,Meiyu Jin,Wanwei Dong,Jia Liu,Zhihong ZhengPubMed 30614095
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC-P | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Tested | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Tested | Not recommended | Tested | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Major component of the transverse filaments of synaptonemal complexes, formed between homologous chromosomes during meiotic prophase. Required for normal assembly of the central element of the synaptonemal complexes. Required for normal centromere pairing during meiosis. Required for normal meiotic chromosome synapsis during oocyte and spermatocyte development and for normal male and female fertility.
Alternative names
SCP1, SYCP1, Synaptonemal complex protein 1, SCP-1, Cancer/testis antigen 8, CT8
Recommended products
Rabbit Recombinant Monoclonal SCP1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP7918
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SCP1 also known as Small Cajal body-specific phosphatase 1 is a protein that acts as a phosphatase. This protein weighs approximately 33 kDa and is expressed in various tissues particularly in the Golgi apparatus and nucleolar regions. SCP1 functions primarily by removing phosphate groups from phosphoserine or phosphothreonine residues on various substrates which regulates their activity and function.
- Biological function summary
SCP1 plays a role in RNA synthesis and processing. It is closely associated with the Cajal body a nuclear organelle involved in RNA biogenesis. Within this complex SCP1 contributes to the maturation of small nuclear ribonucleoproteins (snRNPs) which are essential components of the spliceosomal machinery. This influences how genetic information gets translated into proteins impacting cellular function and responses.
- Pathways
SCP1 is integral to both RNA processing and DNA repair mechanisms. Specifically SCP1 participates in the spliceosome pathway interacting with proteins like SMN and coilin which are vital for spliceosome assembly. SCP1 is also linked to the DNA damage response pathway involving other proteins such as ATM and ATR which signal and repair DNA damage to maintain genomic stability.
- Associated diseases and disorders
SCP1 has shown connections to neurological disorders and cancer. In neurodegenerative conditions such as spinal muscular atrophy SCP1 dysregulation affects the SMN protein impacting snRNP assembly. In cancer altered SCP1 activity has been linked to changes in ATM signaling which can lead to unchecked cell growth and division. Understanding SCP1 interactions in these diseases could provide insights into novel therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-SCP1 antibody [EP7918] (ab175191), expandable thumbnail](https://content.abcam.com/products/images/scp1-antibody-ep7918-ab175191--western-blot-img419938.jpg "Western blot - Anti-SCP1 antibody [EP7918] (ab175191)")
Western blot - Anti-SCP1 antibody [EP7918] (ab175191)
All lanes: Western blot - Anti-SCP1 antibody [EP7918] (ab175191) at 1/5000 dilution
Lane 1: Human testis lysate at 15 µg
Lane 2: Mouse testis lysate at 15 µg
Lane 3: Rat testis lysate at 15 µg
Performed under reducing conditions.
Observed band size: 114 kDa
Exposure time: 60s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (ab175191), expandable thumbnail](https://content.abcam.com/products/images/scp1-antibody-ep7918-ab175191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img419941.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (ab175191)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (ab175191)
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling SCP1 with ab175191 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat testis. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (ab175191), expandable thumbnail](https://content.abcam.com/products/images/scp1-antibody-ep7918-ab175191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img419940.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (ab175191)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (ab175191)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling SCP1 with ab175191 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse testis. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (ab175191), expandable thumbnail](https://content.abcam.com/products/images/scp1-antibody-ep7918-ab175191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img419939.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (ab175191)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (ab175191)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling SCP1 with ab175191 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human testis. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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