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AB284826

Anti-SCP1 antibody [EP7918] - BSA and Azide free

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Rabbit Recombinant Monoclonal SCP1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

SCP1, SYCP1, Synaptonemal complex protein 1, SCP-1, Cancer/testis antigen 8, CT8

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] - BSA and Azide free (AB284826)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] - BSA and Azide free (AB284826)

This data was developed using ab175191, the same antibody clone in a different buffer. Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling SCP1 with ab175191 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human testis. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] - BSA and Azide free (AB284826)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] - BSA and Azide free (AB284826)

This data was developed using ab175191, the same antibody clone in a different buffer. Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling SCP1 with ab175191 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse testis. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] - BSA and Azide free (AB284826)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] - BSA and Azide free (AB284826)

This data was developed using ab175191, the same antibody clone in a different buffer. Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling SCP1 with ab175191 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat testis. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Western blot - Anti-SCP1 antibody [EP7918] - BSA and Azide free (AB284826)
  • WB

Supplier Data

Western blot - Anti-SCP1 antibody [EP7918] - BSA and Azide free (AB284826)

This data was developed using ab175191, the same antibody clone in a different buffer.

All lanes:

Western blot - Anti-SCP1 antibody [EP7918] (<a href='/en-us/products/primary-antibodies/scp1-antibody-ep7918-ab175191'>ab175191</a>) at 1/5000 dilution

Lane 1:

Human testis lysate at 15 µg

Lane 2:

Mouse testis lysate at 15 µg

Lane 3:

Rat testis lysate at 15 µg

Observed band size: 114 kDa

false

Exposure time: 60s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP7918

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat, Mouse

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Secondary Antibodies

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Reactivity data

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Product details

ab284826 is the carrier-free version of ab175191

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCP1 also known as Small Cajal body-specific phosphatase 1 is a protein that acts as a phosphatase. This protein weighs approximately 33 kDa and is expressed in various tissues particularly in the Golgi apparatus and nucleolar regions. SCP1 functions primarily by removing phosphate groups from phosphoserine or phosphothreonine residues on various substrates which regulates their activity and function.
Biological function summary

SCP1 plays a role in RNA synthesis and processing. It is closely associated with the Cajal body a nuclear organelle involved in RNA biogenesis. Within this complex SCP1 contributes to the maturation of small nuclear ribonucleoproteins (snRNPs) which are essential components of the spliceosomal machinery. This influences how genetic information gets translated into proteins impacting cellular function and responses.

Pathways

SCP1 is integral to both RNA processing and DNA repair mechanisms. Specifically SCP1 participates in the spliceosome pathway interacting with proteins like SMN and coilin which are vital for spliceosome assembly. SCP1 is also linked to the DNA damage response pathway involving other proteins such as ATM and ATR which signal and repair DNA damage to maintain genomic stability.

SCP1 has shown connections to neurological disorders and cancer. In neurodegenerative conditions such as spinal muscular atrophy SCP1 dysregulation affects the SMN protein impacting snRNP assembly. In cancer altered SCP1 activity has been linked to changes in ATM signaling which can lead to unchecked cell growth and division. Understanding SCP1 interactions in these diseases could provide insights into novel therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major component of the transverse filaments of synaptonemal complexes, formed between homologous chromosomes during meiotic prophase. Required for normal assembly of the central element of the synaptonemal complexes. Required for normal centromere pairing during meiosis. Required for normal meiotic chromosome synapsis during oocyte and spermatocyte development and for normal male and female fertility.
See full target information SYCP1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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