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Rabbit Recombinant Monoclonal SCP1 antibody. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] (AB303520), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] (AB303520), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] (AB303520), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] (AB303520), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR27085-83] (AB303520), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrICC/IFIP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Tested
Rat
Tested
Tested
Tested
Not recommended
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Rat
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Major component of the transverse filaments of synaptonemal complexes, formed between homologous chromosomes during meiotic prophase (PubMed:16717126). Required for normal assembly of the central element of the synaptonemal complexes (PubMed:15937223). Required for normal centromere pairing during meiosis (PubMed:22761579). Required for normal meiotic chromosome synapsis during oocyte and spermatocyte development and for normal male and female fertility (PubMed:15937223).

Alternative names

Scp1, Sycp1, Synaptonemal complex protein 1, SCP-1

Recommended products

Rabbit Recombinant Monoclonal SCP1 antibody. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27085-83
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SCP1 also known as Small Cajal body-specific phosphatase 1 is a protein that acts as a phosphatase. This protein weighs approximately 33 kDa and is expressed in various tissues particularly in the Golgi apparatus and nucleolar regions. SCP1 functions primarily by removing phosphate groups from phosphoserine or phosphothreonine residues on various substrates which regulates their activity and function.

Biological function summary

SCP1 plays a role in RNA synthesis and processing. It is closely associated with the Cajal body a nuclear organelle involved in RNA biogenesis. Within this complex SCP1 contributes to the maturation of small nuclear ribonucleoproteins (snRNPs) which are essential components of the spliceosomal machinery. This influences how genetic information gets translated into proteins impacting cellular function and responses.

Pathways

SCP1 is integral to both RNA processing and DNA repair mechanisms. Specifically SCP1 participates in the spliceosome pathway interacting with proteins like SMN and coilin which are vital for spliceosome assembly. SCP1 is also linked to the DNA damage response pathway involving other proteins such as ATM and ATR which signal and repair DNA damage to maintain genomic stability.

Associated diseases and disorders

SCP1 has shown connections to neurological disorders and cancer. In neurodegenerative conditions such as spinal muscular atrophy SCP1 dysregulation affects the SMN protein impacting snRNP assembly. In cancer altered SCP1 activity has been linked to changes in ATM signaling which can lead to unchecked cell growth and division. Understanding SCP1 interactions in these diseases could provide insights into novel therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat heart (fresh) tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat heart (PMID: 18425777). The nuclear counterstain was DAPI (Blue). The section was incubated with ab303520 for 60mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat testis (fresh) tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat testis. The nuclear counterstain was DAPI (Blue). The section was incubated with ab303520 for 60mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse heart (fresh) tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing positive staining on mouse heart (PMID: 18425777). The nuclear counterstain was DAPI (Blue). The section was incubated with ab303520 for 60mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse testis (fresh) tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse testis. The nuclear counterstain was DAPI (Blue). The section was incubated with ab303520 for 60mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520)

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: No staining in rat cardiac muscle. The section was incubated with ab303520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520)

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: No staining in mouse cardiac muscle. The section was incubated with ab303520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining in rat testis. The section was incubated with ab303520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR27085-83] (ab303520)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining in mouse testis. The section was incubated with ab303520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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