Anti-SCP1 antibody [EPR27085-83] - BSA and Azide free

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SCP1 antibody [EPR27085-83] - BSA and Azide free (AB303521)

This data was developed using ab303520, the same antibody clone in a different buffer formulation.

Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections Rat testis labelling Lin28A with ab279647 at 1 : 1000 dilution (B), STAT4 with ab284408 at 1 : 500 dilution (C) and SCP1 with ab303520 at 1 : 500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Lin28A (green; Opal™520), anti-STAT4 (magenta; Opal™690) and anti-SCP1 (gray; Opal™570) on rat testis.

Panel B : anti-Lin28A staining undifferentiated spermatogonia in rat testis.

Panel C : ant-STAT4 staining round spermatids (rs) and elongated spermatids (es) in rat testis.

Panel D : ant-SCP1 staining spermatocytes in rat testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab279647, ab284408 and ab303520 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR27085-83] - BSA and Azide free (AB303521)

This data was developed using ab303520, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining in mouse testis. The section was incubated with ab303520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR27085-83] - BSA and Azide free (AB303521)

This data was developed using ab303520, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : No staining in mouse cardiac muscle. The section was incubated with ab303520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR27085-83] - BSA and Azide free (AB303521)

This data was developed using ab303520, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : No staining in rat cardiac muscle. The section was incubated with ab303520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] - BSA and Azide free (AB303521)

This data was developed using ab303520, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse heart (fresh) tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing positive staining on mouse heart (PMID : 18425777). The nuclear counterstain was DAPI (Blue). The section was incubated with ab303520 for 60mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] - BSA and Azide free (AB303521)

This data was developed using ab303520, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat testis (fresh) tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat testis. The nuclear counterstain was DAPI (Blue). The section was incubated with ab303520 for 60mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] - BSA and Azide free (AB303521)

This data was developed using ab303520, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat heart (fresh) tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat heart (PMID : 18425777). The nuclear counterstain was DAPI (Blue). The section was incubated with ab303520 for 60mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SCP1 antibody [EPR27085-83] - BSA and Azide free (AB303521)

This data was developed using ab303520, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse testis (fresh) tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse testis. The nuclear counterstain was DAPI (Blue). The section was incubated with ab303520 for 60mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR27085-83] - BSA and Azide free (AB303521)

This data was developed using ab303520, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling SCP1 with ab303520 at 1/500 (1.074 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining in rat testis. The section was incubated with ab303520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-SCP1 antibody [EPR27085-83] - BSA and Azide free (AB303521)

This data was developed using ab303520, the same antibody clone in a different buffer formulation. Blocking / Diluting buffer and concentration : 5% NFDM/TBST Negative control : heart (PMID : 18425777)

All lanes:

Western blot - Anti-SCP1 antibody [EPR27085-83] (<a href='/en-us/products/primary-antibodies/scp1-antibody-epr27085-83-ab303520'>ab303520</a>) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Rat testis tissue lysate at 20 µg

Lane 4:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 114 kDa

Observed band size: 140 kDa

false

Exposure time: 114s