Anti-SCP1 antibody [EPR29778-50]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR29778-50] (AB324729)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling SCP1 with ab324729 at 1/1000 (0.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse testis. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SCP1 antibody [EPR29778-50] (AB324729)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat testis tissue staining SCP1 with ab324729 at a 1/1000 ( 0.49 μg/ml) dilution, ab323317 anti-Oligodendrocyte Specific Protein used at a 1/500 (1.032 μg/ml) dilution and ab267377 anti-Wilms tumor protein used at a 1/500 ( 0.706 μg/ml) dilution.

Panel A : merged staining of anti-SCP1 (green; Opal™520), anti-Oligodendrocyte Specific Protein (magenta; Opal™690) and anti-Wilms tumor protein (gray; Opal™570) on rat testis.

Panel B : anti-SCP1 staining spermatocytes in rat testis.

Panel C : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in rat testis.

Panel D : anti-Wilms tumor protein staining Sertoli cells in rat testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324729, ab323317 and ab267377 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR29778-50] (AB324729)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling SCP1 with ab324729 at 1/1000 (0.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat cardiac muscle. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR29778-50] (AB324729)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling SCP1 with ab324729 at 1/1000 (0.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse cardiac muscle (PMID : 18425777). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SCP1 antibody [EPR29778-50] (AB324729)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocytes labelling SCP1 with ab324729 at 1/200 (2.45 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing no staining in mouse splenocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

-ve control 1 : ab324729 at a 1/200 dilution followed by ab150120 at a 1/1000 dilution.

-ve control 2 : ab97672 at a 1/100 dilution followed by ab150081 at a 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SCP1 antibody [EPR29778-50] (AB324729)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat splenocytes labelling SCP1 with ab324729 at 1/200 (2.45 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing no staining in rat splenocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

-ve control 1 : ab324729 at a 1/200 dilution followed by ab150120 at a 1/1000 dilution.

-ve control 2 : ab97672 at a 1/100 dilution followed by ab150081 at a 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EPR29778-50] (AB324729)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling SCP1 with ab324729 at 1/1000 (0.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat testis. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SCP1 antibody [EPR29778-50] (AB324729)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat spermatocytes labelling SCP1 with ab324729 at 1/200 (2.45 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing chromosomal staining in rat spermatocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab97672 Anti-SCP3 mouse monoclonal antibody was used to counterstain tubulin at 1/100 (10 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

-ve control 1 : ab324729 at a 1/200 dilution followed by ab150120 at a 1/1000 dilution.

-ve control 2 : ab97672 at a 1/100 dilution followed by ab150081 at a 1/1000 dilution.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SCP1 antibody [EPR29778-50] (AB324729)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse testis tissue staining SCP1 with ab324729 at a 1/1000 ( 0.49 μg/ml) dilution, ab323317 anti-Oligodendrocyte Specific Protein used at a 1/500 (1.032 μg/ml) dilution and ab267377 anti-Wilms tumor protein used at a 1/500 ( 0.706 μg/ml) dilution.

Panel A : merged staining of anti-SCP1 (green; Opal™520), anti-Oligodendrocyte Specific Protein (magenta; Opal™690) and anti-Wilms tumor protein (gray; Opal™570) on mouse testis.

Panel B : anti-SCP1 staining spermatocytes in mouse testis.

Panel C : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in mouse testis.

Panel D : anti-Wilms tumor protein staining Sertoli cells in mouse testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324729, ab323317 and ab267377 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SCP1 antibody [EPR29778-50] (AB324729)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse spermatocytes labelling SCP1 with ab324729 at 1/200 (2.45 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing chromosomal staining in mouse spermatocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab97672 Anti-SCP3 mouse monoclonal antibody was used to counterstain tubulin at 1/100 (10 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

-ve control 1 : ab324729 at a 1/200 dilution followed by ab150120 at a 1/1000 dilution.

-ve control 2 : ab97672 at a 1/100 dilution followed by ab150081 at a 1/1000 dilution.

• WB

Supplier Data

Western blot - Anti-SCP1 antibody [EPR29778-50] (AB324729)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen, heart, lung (PMID : 18425777).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SCP1 antibody [EPR29778-50] (ab324729) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lane 4:

Mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140 kDa,115 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-SCP1 antibody [EPR29778-50] (AB324729)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen, heart, lung (PMID : 18425777).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SCP1 antibody [EPR29778-50] (ab324729) at 1/1000 dilution

Lane 1:

Rat testis tissue lysate at 20 µg

Lane 2:

Rat heart tissue lysate at 20 µg

Lane 3:

Rat spleen tissue lysate at 20 µg

Lane 4:

Rat lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140 kDa,36 kDa

false

Exposure time: 15s