AB181746

Anti-SCP3 antibody [6F9C5]

Name: Anti-SCP3 antibody [6F9C5] (ab181746) | Abcam
Brand: Abcam Limited
SKU: AB181746
Price: 500 USD
Availability: InStock

(1 Review)

(5 Publications)

Mouse Monoclonal SCP3 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Recombinant full length protein samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SYCP3 aa 1-150.

View Alternative Names

SCP3, SYCP3, Synaptonemal complex protein 3, SCP-3

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [6F9C5] (AB181746)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [6F9C5] (AB181746)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling SCP3 with ab181746 at 1/200.

Flow Cytometry - Anti-SCP3 antibody [6F9C5] (AB181746)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-SCP3 antibody [6F9C5] (AB181746)

Flow Cytometrical analysis of Jurkat cells labeling SCP3 with ab181746 at 1/200 (green) compared to a negative control antibody (red).

Immunocytochemistry/ Immunofluorescence - Anti-SCP3 antibody [6F9C5] (AB181746)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SCP3 antibody [6F9C5] (AB181746)

Immunofluorescent analysis of HepG2 cells labeling SPC3 with ab181746 at 1/200 (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [6F9C5] (AB181746)

Immunohistochemical analysis of paraffin embedded Human cervical cancer tissue labeling SCP3 with ab181746 at 1/200.

Supplier Data

Western blot - Anti-SCP3 antibody [6F9C5] (AB181746)

All lanes:

Western blot - Anti-SCP3 antibody [6F9C5] (ab181746) at 1/500 dilution

Lane 1:

non-transfected HEK293 cell lysate

Lane 2:

SCP3 (aa 27-128)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 28 kDa

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Supplier Data

Western blot - Anti-SCP3 antibody [6F9C5] (AB181746)

All lanes:

Western blot - Anti-SCP3 antibody [6F9C5] (ab181746) at 1/500 dilution

All lanes:

SCP3 recombinant protein

Predicted band size: 28 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6F9C5

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human SYCP3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q8IZU3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to supernatant. Lot no's high than GR160891-17 are from Tissue Culture Supernatant

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SCP3 protein also known as Synaptonemal complex protein 3 and COR1 plays an important role in cell division. It is a component of the synaptonemal complex which is essential during meiosis for chromosome pairing and recombination. SCP3 has a molecular mass of approximately 30 kDa. Researchers observe its expression in germline cells where it facilitates the accurate segregation of homologous chromosomes during the prophase I stage of meiosis.

Biological function summary

SCP3 serves an important function in organizing the scaffold of the synaptonemal complex a highly organized proteinaceous framework. This structure helps in stabilizing the pairing of homologous chromosomes and promoting synapsis. SCP3 does not work alone; it operates as part of a larger complex with other synaptonemal complex components including SCP1 and SCP2. This coordination ensures the successful exchange of genetic material between chromosomes enhancing genetic diversity in gametes.

Pathways

SCP3 integrates into the meiotic recombination process essential for gametogenesis. It aligns with the DNA repair pathway significantly impacting homologous recombination. Proteins like RAD51 and DMC1 are part of these pathways interacting indirectly with SCP3 to facilitate recombination repair processes. SCP3's role helps maintain genome integrity by ensuring faithful chromosome segregation which is critical in producing viable gametes.

SCP3 implicates reproductive issues and genetic schizophrenia predispositions. Abnormal or disrupted function of SCP3 often leads to infertility due to failed meiosis impacting gamete viability. This malfunction may associate with proteins like SPO11 which initiates meiotic recombination. Alterations in SCP3's function or expression have been studied for their potential connections to schizophrenia suggesting a role in neurological development related to errors during meiosis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Required for centromere pairing during meiosis in male germ cells (By similarity). Required for normal meiosis during spermatogenesis and male fertility (PubMed : 14643120). Plays a lesser role in female fertility. Required for efficient phosphorylation of HORMAD1 and HORMAD2 (By similarity).

See full target information SYCP3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Stem cell reviews and reports 19:1067-1081 PubMed36735215

2023

Oocyte Arrested at Metaphase II Stage were Derived from Human Pluripotent Stem Cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Yu,Ning Wang,Xiang Wang,Hehe Ren,Yanping Zhang,Yingxin Zhang,Yikai Qiu,Hongyan Wang,Guoping Wang,Xiuying Pei,Ping Chen,Yahui Ren,Chunfang Ha,Li Wang,Huayan Wang

Frontiers in cell and developmental biology 10:862506 PubMed35493072

2022

MFN2 Deficiency Impairs Mitochondrial Functions and PPAR Pathway During Spermatogenesis and Meiosis in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tianren Wang,Yuan Xiao,Zhe Hu,Jingkai Gu,Renwu Hua,Zhuo Hai,Xueli Chen,Jian V Zhang,Zhiying Yu,Ting Wu,William S B Yeung,Kui Liu,Chenxi Guo

Toxicological sciences : an official journal of the Society of Toxicology 173:53-64 PubMed31593228

2019

Evaluation of Resmethrin Toxicity to Neonatal Testes in Organ Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun-Jung Park,Won-Young Lee,Mingtian Zhang,Kwon-Ho Hong,Chankyu Park,Jin-Hoi Kim,Hyuk Song

Chromosoma 128:369-383 PubMed31256246

2019

SIRT7 promotes chromosome synapsis during prophase I of female meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Berta N Vazquez,Cecilia S Blengini,Yurdiana Hernandez,Lourdes Serrano,Karen Schindler

Biology of reproduction 90:73 PubMed24571984

2014

Human amniotic fluid stem cells possess the potential to differentiate into primordial follicle oocytes in vitro.

Applications

Unspecified application

Species

Human

Xiaoli Yu,Ning Wang,Rong Qiang,Qianhui Wan,Mingming Qin,Shuai Chen,Huayan Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SCP3 antibody [6F9C5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [6F9C5] (AB181746)

Flow Cytometry - Anti-SCP3 antibody [6F9C5] (AB181746)

Immunocytochemistry/ Immunofluorescence - Anti-SCP3 antibody [6F9C5] (AB181746)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [6F9C5] (AB181746)

Western blot - Anti-SCP3 antibody [6F9C5] (AB181746)

Western blot - Anti-SCP3 antibody [6F9C5] (AB181746)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise