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AB235254

Anti-SCP3 antibody

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(1 Publication)

Rabbit Polyclonal SCP3 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

Scp3, Sycp3, Synaptonemal complex protein 3, SCP-3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody (AB235254)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody (AB235254)

Negative control : Immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue sections labeling SCP3 with ab235254 at a 1/100 dilution (30mins at RT), followed by a ready to use anti-Rabbit poly-HRP-IgG secondary (8mins at RT).

Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody (AB235254)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody (AB235254)

Immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse testis tissue sections labeling SCP3 with ab235254 at a 1/100 dilution (30mins at RT), followed by a ready to use anti-Rabbit poly-HRP-IgG secondary (8mins at RT).

Counterstained with hematoxylin.

Western blot - Anti-SCP3 antibody (AB235254)
  • WB

Supplier Data

Western blot - Anti-SCP3 antibody (AB235254)

Blocking buffer : 5% BLOTTO.

All lanes:

Western blot - Anti-SCP3 antibody (ab235254) at 5 µg/mL

Lane 1:

Mouse testis whole tissue lysate at 35 µg

Lane 2:

Human testis whole tissue lysate at 35 µg

Lane 3:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 35 µg

Secondary

All lanes:

Goat anti-Rabbit IgG at 1/70000 dilution

Predicted band size: 28 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SCP3 protein also known as Synaptonemal complex protein 3 and COR1 plays an important role in cell division. It is a component of the synaptonemal complex which is essential during meiosis for chromosome pairing and recombination. SCP3 has a molecular mass of approximately 30 kDa. Researchers observe its expression in germline cells where it facilitates the accurate segregation of homologous chromosomes during the prophase I stage of meiosis.
Biological function summary

SCP3 serves an important function in organizing the scaffold of the synaptonemal complex a highly organized proteinaceous framework. This structure helps in stabilizing the pairing of homologous chromosomes and promoting synapsis. SCP3 does not work alone; it operates as part of a larger complex with other synaptonemal complex components including SCP1 and SCP2. This coordination ensures the successful exchange of genetic material between chromosomes enhancing genetic diversity in gametes.

Pathways

SCP3 integrates into the meiotic recombination process essential for gametogenesis. It aligns with the DNA repair pathway significantly impacting homologous recombination. Proteins like RAD51 and DMC1 are part of these pathways interacting indirectly with SCP3 to facilitate recombination repair processes. SCP3's role helps maintain genome integrity by ensuring faithful chromosome segregation which is critical in producing viable gametes.

SCP3 implicates reproductive issues and genetic schizophrenia predispositions. Abnormal or disrupted function of SCP3 often leads to infertility due to failed meiosis impacting gamete viability. This malfunction may associate with proteins like SPO11 which initiates meiotic recombination. Alterations in SCP3's function or expression have been studied for their potential connections to schizophrenia suggesting a role in neurological development related to errors during meiosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase (PubMed : 11311943, PubMed : 22761579). Required for centromere pairing during meiosis in male germ cells (PubMed : 22761579). Required for normal meiosis during spermatogenesis and male fertility (PubMed : 10678170). Plays a lesser role in female fertility (PubMed : 10678170, PubMed : 12004129). Required for efficient phosphorylation of HORMAD1 and HORMAD2 (PubMed : 22346761).
See full target information Sycp3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 6:eaba7589 PubMed32582856

2020

Self-assembled cGAMP-STINGΔTM signaling complex as a bioinspired platform for cGAMP delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Yanpu He,Celestine Hong,Emily Z Yan,Samantha J Fletcher,Ge Zhu,Mengdi Yang,Yingzhong Li,Xin Sun,Darrell J Irvine,Jiahe Li,Paula T Hammond
View all publications
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Product promise

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