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Anti-SCP3 antibody [Cor 10G11/7] ab97672 is a mouse monoclonal antibody that is used in SCP3 western blotting and IHC. Suitable for mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone Cor 10G11/7 is the most widely used clone for SCP3 on the market


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (AB97672), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (AB97672), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (AB97672), expandable thumbnail
  • Western blot - Anti-SCP3 antibody [Cor 10G11/7] (AB97672), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (AB97672), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Mouse
Tested
Tested
Rat
Tested
Tested
Hamster
Predicted
Predicted
Zebrafish
Predicted
Predicted

Tested
Tested

Species
Mouse
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Hamster, Zebrafish
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Hamster, Zebrafish
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase (PubMed:11311943, PubMed:22761579). Required for centromere pairing during meiosis in male germ cells (PubMed:22761579). Required for normal meiosis during spermatogenesis and male fertility (PubMed:10678170). Plays a lesser role in female fertility (PubMed:10678170, PubMed:12004129). Required for efficient phosphorylation of HORMAD1 and HORMAD2 (PubMed:22346761).

Alternative names

Scp3, Sycp3, Synaptonemal complex protein 3, SCP-3

Recommended products

Anti-SCP3 antibody [Cor 10G11/7] ab97672 is a mouse monoclonal antibody that is used in SCP3 western blotting and IHC. Suitable for mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone Cor 10G11/7 is the most widely used clone for SCP3 on the market

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
Cor 10G11/7
Purification technique
Affinity purification Protein A
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product has switched from a hybridoma to recombinant production method on 26 September 2022.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The SCP3 protein also known as Synaptonemal complex protein 3 and COR1 plays an important role in cell division. It is a component of the synaptonemal complex which is essential during meiosis for chromosome pairing and recombination. SCP3 has a molecular mass of approximately 30 kDa. Researchers observe its expression in germline cells where it facilitates the accurate segregation of homologous chromosomes during the prophase I stage of meiosis.

Biological function summary

SCP3 serves an important function in organizing the scaffold of the synaptonemal complex a highly organized proteinaceous framework. This structure helps in stabilizing the pairing of homologous chromosomes and promoting synapsis. SCP3 does not work alone; it operates as part of a larger complex with other synaptonemal complex components including SCP1 and SCP2. This coordination ensures the successful exchange of genetic material between chromosomes enhancing genetic diversity in gametes.

Pathways

SCP3 integrates into the meiotic recombination process essential for gametogenesis. It aligns with the DNA repair pathway significantly impacting homologous recombination. Proteins like RAD51 and DMC1 are part of these pathways interacting indirectly with SCP3 to facilitate recombination repair processes. SCP3's role helps maintain genome integrity by ensuring faithful chromosome segregation which is critical in producing viable gametes.

Associated diseases and disorders

SCP3 implicates reproductive issues and genetic schizophrenia predispositions. Abnormal or disrupted function of SCP3 often leads to infertility due to failed meiosis impacting gamete viability. This malfunction may associate with proteins like SPO11 which initiates meiotic recombination. Alterations in SCP3's function or expression have been studied for their potential connections to schizophrenia suggesting a role in neurological development related to errors during meiosis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672), expandable thumbnail
    Shawki et al PLoS One. 2018 Jan 11;13(1):e0190800. doi: 10.1371/journal.pone.0190800. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)

    This image was generated from a previous batch made using the hybridoma production method.

    Localization of MAFB in adult mouse testis.

    Double immunostaining of MAFB with SCP3 (ab97672 at a 1/400 dilution, O/N +4°C). Nuclei were counterstained with DAPI. The color of each marker is indicated above the images. All seminiferous tubules shown represent stage VII. US; undifferentiated spermatogonia. DS; differentiated spermatogonia. P; pachytene spermatocytes. Sp; spermatids. S; Sertoli cells. L; Leydig cells. MAFB was specifically detected in Leydig cells, Sertoli cells, and pachytene spermatocytes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672), expandable thumbnail
    This image is courtesy of a customer review submitted by Qin Wen

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)

    This image was generated from a previous batch made using the hybridoma production method.

    ab97672 staining SCP3 in mouse adult testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a 0.01M citrate buffer. Samples were incubated with primary antibody (1/200 in 1% BSA) for 1 hour at 22°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672), expandable thumbnail
    This image is courtesy of a customer review by Zachary Yu-Ching Lin.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)

    This image was generated from a previous batch made using the hybridoma production method.

    IHC-P image of SCP3 staining with ab97672 on tissue sections from adult mouse testis.

    The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab97672 (1/200 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor®488 conjugated goat anti-mouse polyclonal, used at a 1/500 dilution.

  • Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672), expandable thumbnail

    Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)

    All lanes: Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672) at 1/1000 dilution

    Lane 1: Rat testis tissue lysate at 20 µg

    Lane 2: Mouse testis tissue lysate at 20 µg

    Developed using the ECL technique.

    Observed band size: 27 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SCP3 with ab97672 at 1/4000 dilution followed by Leica DS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab97672 for 30 mins at room temperature and blocking mouse IgG by specific antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913, 1:1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling SCP3 with ab97672 at 1/4000 dilution followed by Leica DS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab97672 for 30 mins at room temperature and blocking mouse IgG by specific antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913, 1:1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com