Anti-SCP3 antibody [Cor 10G11/7]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (AB97672)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SCP3 with ab97672 at 1/4000 dilution followed by Leica DS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab97672 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab125913, 1 : 1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (AB97672)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling SCP3 with ab97672 at 1/4000 dilution followed by Leica DS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab97672 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab125913, 1 : 1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

• IHC-P

AbReview31982****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (AB97672)

This image was generated from a previous batch made using the hybridoma production method.

IHC-P image of SCP3 staining with ab97672 on tissue sections from adult mouse testis.

The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab97672 (1/200 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor^®488 conjugated goat anti-mouse polyclonal, used at a 1/500 dilution.

This image is courtesy of an abreview by Zachary Yu-Ching Lin.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SCP3 antibody [Cor 10G11/7] (AB97672)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse spermatocytes labelling SCP1 with ab324729 at 1/200 (2.45 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing chromosomal staining in mouse spermatocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab97672 Anti-SCP3 mouse monoclonal antibody was used to counterstain tubulin at 1/100 (10 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

-ve control 1 : ab324729 at a 1/200 dilution followed by ab150120 at a 1/1000 dilution.

-ve control 2 : ab97672 at a 1/100 dilution followed by ab150081 at a 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SCP3 antibody [Cor 10G11/7] (AB97672)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat spermatocytes labelling SCP1 with ab324729 at 1/200 (2.45 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing chromosomal staining in rat spermatocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab97672 Anti-SCP3 mouse monoclonal antibody was used to counterstain tubulin at 1/100 (10 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

-ve control 1 : ab324729 at a 1/200 dilution followed by ab150120 at a 1/1000 dilution.

-ve control 2 : ab97672 at a 1/100 dilution followed by ab150081 at a 1/1000 dilution.

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (AB97672)

This image was generated from a previous batch made using the hybridoma production method.

Localization of MAFB in adult mouse testis.

Double immunostaining of MAFB with SCP3 (ab97672 at a 1/400 dilution, O/N +4°C). Nuclei were counterstained with DAPI. The color of each marker is indicated above the images. All seminiferous tubules shown represent stage VII. US; undifferentiated spermatogonia. DS; differentiated spermatogonia. P; pachytene spermatocytes. Sp; spermatids. S; Sertoli cells. L; Leydig cells. MAFB was specifically detected in Leydig cells, Sertoli cells, and pachytene spermatocytes.

Shawki et al PLoS One. 2018 Jan 11;13(1):e0190800. doi: 10.1371/journal.pone.0190800. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• IHC-P

AbReview35107****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (AB97672)

This image was generated from a previous batch made using the hybridoma production method.

ab97672 staining SCP3 in mouse adult testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a 0.01M citrate buffer. Samples were incubated with primary antibody (1/200 in 1% BSA) for 1 hour at 22°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Qin Wen

• WB

Lab

Western blot - Anti-SCP3 antibody [Cor 10G11/7] (AB97672)

All lanes:

Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672) at 1/1000 dilution

Lane 1:

Rat testis tissue lysate at 20 µg

Lane 2:

Mouse testis tissue lysate at 20 µg

Observed band size: 27 kDa

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