Mouse Recombinant Monoclonal SCP3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat samples.
Images
![Western blot - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (AB264093), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-cor-10g11-7-bsa-and-azide-free-ab264093--western-blot-img400262.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (AB264093)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (AB264093), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-cor-10g11-7-bsa-and-azide-free-ab264093--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401262.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (AB264093)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (AB264093), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-cor-10g11-7-bsa-and-azide-free-ab264093--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401261.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (AB264093)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Not recommended | Not recommended |
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
| Hamster | Not recommended | Not recommended |
| Zebrafish | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Hamster | Dilution info - | Notes - |
Species Zebrafish | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Hamster, Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase (PubMed:11311943, PubMed:22761579). Required for centromere pairing during meiosis in male germ cells (PubMed:22761579). Required for normal meiosis during spermatogenesis and male fertility (PubMed:10678170). Plays a lesser role in female fertility (PubMed:10678170, PubMed:12004129). Required for efficient phosphorylation of HORMAD1 and HORMAD2 (PubMed:22346761).
Alternative names
Scp3, Sycp3, Synaptonemal complex protein 3, SCP-3
Recommended products
Mouse Recombinant Monoclonal SCP3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- Cor 10G11/7
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab264093 is the carrier-free version of Anti-SCP3 antibody [Cor 10G11/7] ab97672.
This product has switched from a hybridoma to recombinant production method on 26 September 2022.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SCP3 protein also known as Synaptonemal complex protein 3 and COR1 plays an important role in cell division. It is a component of the synaptonemal complex which is essential during meiosis for chromosome pairing and recombination. SCP3 has a molecular mass of approximately 30 kDa. Researchers observe its expression in germline cells where it facilitates the accurate segregation of homologous chromosomes during the prophase I stage of meiosis.
- Biological function summary
SCP3 serves an important function in organizing the scaffold of the synaptonemal complex a highly organized proteinaceous framework. This structure helps in stabilizing the pairing of homologous chromosomes and promoting synapsis. SCP3 does not work alone; it operates as part of a larger complex with other synaptonemal complex components including SCP1 and SCP2. This coordination ensures the successful exchange of genetic material between chromosomes enhancing genetic diversity in gametes.
- Pathways
SCP3 integrates into the meiotic recombination process essential for gametogenesis. It aligns with the DNA repair pathway significantly impacting homologous recombination. Proteins like RAD51 and DMC1 are part of these pathways interacting indirectly with SCP3 to facilitate recombination repair processes. SCP3's role helps maintain genome integrity by ensuring faithful chromosome segregation which is critical in producing viable gametes.
- Associated diseases and disorders
SCP3 implicates reproductive issues and genetic schizophrenia predispositions. Abnormal or disrupted function of SCP3 often leads to infertility due to failed meiosis impacting gamete viability. This malfunction may associate with proteins like SPO11 which initiates meiotic recombination. Alterations in SCP3's function or expression have been studied for their potential connections to schizophrenia suggesting a role in neurological development related to errors during meiosis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-cor-10g11-7-bsa-and-azide-free-ab264093--western-blot-img400262.jpg "Western blot - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)")
Western blot - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)
All lanes: Western blot - Anti-SCP3 antibody [Cor 10G11/7] (Anti-SCP3 antibody [Cor 10G11/7] ab97672) at 1/1000 dilution
Lane 1: Rat testis tissue lysate at 20 µg
Lane 2: Mouse testis tissue lysate at 20 µg
Developed using the ECL technique.
Observed band size: 27 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-cor-10g11-7-bsa-and-azide-free-ab264093--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401262.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SCP3 with Anti-SCP3 antibody [Cor 10G11/7] ab97672 at 1/4000 dilution followed by Leica DS9800 (Bond™ Polymer Refine Detection). The section was incubated with Anti-SCP3 antibody [Cor 10G11/7] ab97672 for 30 mins at room temperature and blocking mouse IgG by specific antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913, 1:1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-cor-10g11-7-bsa-and-azide-free-ab264093--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401261.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling SCP3 with Anti-SCP3 antibody [Cor 10G11/7] ab97672 at 1/4000 dilution followed by Leica DS9800 (Bond™ Polymer Refine Detection). The section was incubated with Anti-SCP3 antibody [Cor 10G11/7] ab97672 for 30 mins at room temperature and blocking mouse IgG by specific antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913, 1:1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
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