Rabbit Recombinant Monoclonal SCP3 antibody. Carrier free. Suitable for IHC-P, WB, IHC-Fr and reacts with Mouse samples.
Images
![Western blot - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (AB314752), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-epr27086-10-bsa-and-azide-free-ab314752--western-blot-img422464.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (AB314752)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (AB314752), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-epr27086-10-bsa-and-azide-free-ab314752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422222.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (AB314752)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (AB314752), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-epr27086-10-bsa-and-azide-free-ab314752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422221.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (AB314752)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (AB314752), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-epr27086-10-bsa-and-azide-free-ab314752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422220.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (AB314752)")
![Immunohistochemistry (Frozen sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (AB314752), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-epr27086-10-bsa-and-azide-free-ab314752--immunohistochemistry-frozen-sections-img422156.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (AB314752)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | IHC-Fr | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested |
| Rat | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Required for centromere pairing during meiosis in male germ cells (By similarity). Required for normal meiosis during spermatogenesis and male fertility (PubMed:14643120). Plays a lesser role in female fertility. Required for efficient phosphorylation of HORMAD1 and HORMAD2 (By similarity).
Alternative names
SCP3, SYCP3, Synaptonemal complex protein 3, SCP-3
Recommended products
Rabbit Recombinant Monoclonal SCP3 antibody. Carrier free. Suitable for IHC-P, WB, IHC-Fr and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27086-10
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab314752 is the carrier-free version of Anti-SCP3 antibody [EPR27086-10] ab314751.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SCP3 protein also known as Synaptonemal complex protein 3 and COR1 plays an important role in cell division. It is a component of the synaptonemal complex which is essential during meiosis for chromosome pairing and recombination. SCP3 has a molecular mass of approximately 30 kDa. Researchers observe its expression in germline cells where it facilitates the accurate segregation of homologous chromosomes during the prophase I stage of meiosis.
- Biological function summary
SCP3 serves an important function in organizing the scaffold of the synaptonemal complex a highly organized proteinaceous framework. This structure helps in stabilizing the pairing of homologous chromosomes and promoting synapsis. SCP3 does not work alone; it operates as part of a larger complex with other synaptonemal complex components including SCP1 and SCP2. This coordination ensures the successful exchange of genetic material between chromosomes enhancing genetic diversity in gametes.
- Pathways
SCP3 integrates into the meiotic recombination process essential for gametogenesis. It aligns with the DNA repair pathway significantly impacting homologous recombination. Proteins like RAD51 and DMC1 are part of these pathways interacting indirectly with SCP3 to facilitate recombination repair processes. SCP3's role helps maintain genome integrity by ensuring faithful chromosome segregation which is critical in producing viable gametes.
- Associated diseases and disorders
SCP3 implicates reproductive issues and genetic schizophrenia predispositions. Abnormal or disrupted function of SCP3 often leads to infertility due to failed meiosis impacting gamete viability. This malfunction may associate with proteins like SPO11 which initiates meiotic recombination. Alterations in SCP3's function or expression have been studied for their potential connections to schizophrenia suggesting a role in neurological development related to errors during meiosis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-epr27086-10-bsa-and-azide-free-ab314752--western-blot-img422464.jpg "Western blot - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752)")
Western blot - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752)
SCP3 Western blot staining using rabbit Anti-SCP3 antibody
This data was developed using Anti-SCP3 antibody [EPR27086-10] ab314751, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver (PMID: 14643120; PMID: 31671664), lung (PMID: 14643120; PMID: 31671664). The molecular weight observed is consistent with what has been described in the literature (PMID: 32232159).In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.All lanes: Western blot - Anti-SCP3 antibody [EPR27086-10] (Anti-SCP3 antibody [EPR27086-10] ab314751) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 20 µg
Lane 2: Mouse lung tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 30 kDa, 33 kDa
Exposure time: 180s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-epr27086-10-bsa-and-azide-free-ab314752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422222.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752)
This data was developed using Anti-SCP3 antibody [EPR27086-10] ab314751, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling SCP3 with Anti-SCP3 antibody [EPR27086-10] ab314751 at 1/1000 (0.514 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse lung. The section was incubated with Anti-SCP3 antibody [EPR27086-10] ab314751 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-epr27086-10-bsa-and-azide-free-ab314752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422221.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752)
This data was developed using Anti-SCP3 antibody [EPR27086-10] ab314751, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SCP3 with Anti-SCP3 antibody [EPR27086-10] ab314751 at 1/1000 (0.514 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse liver. The section was incubated with Anti-SCP3 antibody [EPR27086-10] ab314751 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-epr27086-10-bsa-and-azide-free-ab314752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422220.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752)
This data was developed using Anti-SCP3 antibody [EPR27086-10] ab314751, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SCP3 with Anti-SCP3 antibody [EPR27086-10] ab314751 at 1/1000 (0.514 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse testis. The section was incubated with Anti-SCP3 antibody [EPR27086-10] ab314751 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Frozen sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-epr27086-10-bsa-and-azide-free-ab314752--immunohistochemistry-frozen-sections-img422156.jpg "Immunohistochemistry (Frozen sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752)")
Immunohistochemistry (Frozen sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752)
This data was developed using Anti-SCP3 antibody [EPR27086-10] ab314751, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse testis (fresh) tissue labeling SCP3 with Anti-SCP3 antibody [EPR27086-10] ab314751 at 1/500 (1.028 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Confocal image showing positive staining on mouse testis. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-SCP3 antibody [EPR27086-10] ab314751 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.![Immunohistochemistry (Frozen sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-epr27086-10-bsa-and-azide-free-ab314752--immunohistochemistry-frozen-sections-img422157.jpg "Immunohistochemistry (Frozen sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752)")
Immunohistochemistry (Frozen sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752)
This data was developed using Anti-SCP3 antibody [EPR27086-10] ab314751, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung (fresh) tissue labeling SCP3 with Anti-SCP3 antibody [EPR27086-10] ab314751 at 1/500 (1.028 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Negative control: confocal image showing no staining on mouse lung. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-SCP3 antibody [EPR27086-10] ab314751 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
![Immunohistochemistry (Frozen sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752), expandable thumbnail](https://content.abcam.com/products/images/scp3-antibody-epr27086-10-bsa-and-azide-free-ab314752--immunohistochemistry-frozen-sections-img422157.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-SCP3 antibody [EPR27086-10] - BSA and Azide free (ab314752)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com