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Rabbit Recombinant Monoclonal SCP3 antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (AB314313), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (AB314313), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (AB314313), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (AB314313), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (AB314313), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBIP
Human
Tested
Tested
Not recommended
Mouse
Tested
Tested
Not recommended
Rat
Tested
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Required for centromere pairing during meiosis in male germ cells (By similarity). Required for normal meiosis during spermatogenesis and male fertility (PubMed:14643120). Plays a lesser role in female fertility. Required for efficient phosphorylation of HORMAD1 and HORMAD2 (By similarity).

    Alternative names

    Synaptonemal complex protein 3, SCP-3, SCP3, SYCP3

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    Rabbit Recombinant Monoclonal SCP3 antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples.

    Alternative names

    Synaptonemal complex protein 3, SCP-3, SCP3, SYCP3

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Clone number

    EPR27086-101

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate short-term storage duration

    1-2 weeks

    Appropriate short-term storage conditions

    +4°C

    Appropriate long-term storage conditions

    -20°C

    Aliquoting information

    Upon delivery aliquot

    Storage information

    Avoid freeze / thaw cycle

    Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Supplementary info

    Activity summary

    The SCP3 protein also known as Synaptonemal complex protein 3 and COR1 plays an important role in cell division. It is a component of the synaptonemal complex which is essential during meiosis for chromosome pairing and recombination. SCP3 has a molecular mass of approximately 30 kDa. Researchers observe its expression in germline cells where it facilitates the accurate segregation of homologous chromosomes during the prophase I stage of meiosis.

    Biological function summary

    SCP3 serves an important function in organizing the scaffold of the synaptonemal complex a highly organized proteinaceous framework. This structure helps in stabilizing the pairing of homologous chromosomes and promoting synapsis. SCP3 does not work alone; it operates as part of a larger complex with other synaptonemal complex components including SCP1 and SCP2. This coordination ensures the successful exchange of genetic material between chromosomes enhancing genetic diversity in gametes.

    Pathways

    SCP3 integrates into the meiotic recombination process essential for gametogenesis. It aligns with the DNA repair pathway significantly impacting homologous recombination. Proteins like RAD51 and DMC1 are part of these pathways interacting indirectly with SCP3 to facilitate recombination repair processes. SCP3's role helps maintain genome integrity by ensuring faithful chromosome segregation which is critical in producing viable gametes.

    Associated diseases and disorders

    SCP3 implicates reproductive issues and genetic schizophrenia predispositions. Abnormal or disrupted function of SCP3 often leads to infertility due to failed meiosis impacting gamete viability. This malfunction may associate with proteins like SPO11 which initiates meiotic recombination. Alterations in SCP3's function or expression have been studied for their potential connections to schizophrenia suggesting a role in neurological development related to errors during meiosis.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    10 product images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313)

      Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Negative control: no staining on rat lung. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
      Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313)

      Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Negative control: no staining on rat liver. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
      Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313)

      Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Negative control: no staining on mouse lung. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
      Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313)

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Negative control: no staining on mouse liver. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
      Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313)

      Immunohistochemical analysis of paraffin-embedded human lung tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Negative control: no staining on human lung. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
      Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313)

      Immunohistochemical analysis of paraffin-embedded human liver tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Negative control: no staining on human liver. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
      Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313)

      Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Positive staining on rat testis. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
      Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313)

      Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on mouse testis. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
      Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] (ab314313)

      Immunohistochemical analysis of paraffin-embedded human testis tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Positive staining on human testis. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
      Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    • Western blot - Anti-SCP3 antibody [EPR27086-101] (ab314313), expandable thumbnail

      Western blot - Anti-SCP3 antibody [EPR27086-101] (ab314313)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      Negative control: liver (PMID: 14643120; PMID: 31671664), lung (PMID: 14643120; PMID: 31671664).
      The molecular weight observed is consistent with what has been described in the literature (PMID: 32232159).
      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

      All lanes: Western blot - Anti-SCP3 antibody [EPR27086-101] (ab314313) at 1/1000 dilution

      Lane 1: Human testis tissue lysate at 60 µg

      Lane 2: Human lung tissue lysate at 60 µg

      Lane 3: Human liver tissue lysate at 60 µg

      Lane 4: Mouse testis tissue lysate at 60 µg

      Lane 5: Mouse lung tissue lysate at 60 µg

      Lane 6: Mouse liver tissue lysate at 60 µg

      Lane 7: Rat testis tissue lysate at 60 µg

      Lane 8: Rat lung tissue lysate at 60 µg

      Lane 9: Rat liver tissue lysate at 60 µg

      Secondary

      All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Observed band size: 30 kDa, 33 kDa

      Exposure time: 180s

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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