Anti-SCP3 antibody [EPR27086-101] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] - BSA and Azide free (AB314314)

This data was developed using ab314313, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human testis tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on human testis. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] - BSA and Azide free (AB314314)

This data was developed using ab314313, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human lung tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control : no staining on human lung. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] - BSA and Azide free (AB314314)

This data was developed using ab314313, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human liver tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control : no staining on human liver. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] - BSA and Azide free (AB314314)

This data was developed using ab314313, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control : no staining on rat liver. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] - BSA and Azide free (AB314314)

This data was developed using ab314313, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on rat testis. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] - BSA and Azide free (AB314314)

This data was developed using ab314313, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on mouse testis. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] - BSA and Azide free (AB314314)

This data was developed using ab314313, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control : no staining on mouse lung. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] - BSA and Azide free (AB314314)

This data was developed using ab314313, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control : no staining on mouse liver. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [EPR27086-101] - BSA and Azide free (AB314314)

This data was developed using ab314313, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control : no staining on rat lung. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• WB

Supplier Data

Western blot - Anti-SCP3 antibody [EPR27086-101] - BSA and Azide free (AB314314)

This data was developed using ab314313, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : liver (PMID : 14643120; PMID : 31671664), lung (PMID : 14643120; PMID : 31671664). The molecular weight observed is consistent with what has been described in the literature (PMID : 32232159). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SCP3 antibody [EPR27086-101] (<a href='/en-us/products/primary-antibodies/scp3-antibody-epr27086-101-ab314313'>ab314313</a>) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 60 µg

Lane 2:

Human lung tissue lysate at 60 µg

Lane 3:

Human liver tissue lysate at 60 µg

Lane 4:

Mouse testis tissue lysate at 60 µg

Lane 5:

Mouse lung tissue lysate at 60 µg

Lane 6:

Mouse liver tissue lysate at 60 µg

Lane 7:

Rat testis tissue lysate at 60 µg

Lane 8:

Rat lung tissue lysate at 60 µg

Lane 9:

Rat liver tissue lysate at 60 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 30 kDa,33 kDa

false

Exposure time: 180s