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AB96318

Anti-Scramblase 1 antibody

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(1 Publication)

Rabbit Polyclonal Scramblase 1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PLSCR1 aa 1 to C-terminus.
1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scramblase 1 antibody (AB96318)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scramblase 1 antibody (AB96318)

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using ab96318 at 1/500 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PLSCR1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15162

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Scramblase 1 also known as PLSCR1 is a protein involved in the translocation of phospholipids between the inner and outer leaflets of the lipid bilayer. This protein has a molecular mass of approximately 37 kDa. PLSCR1 is found in various tissues and cells with notable expression in the hematopoietic system. As a type II membrane protein Scramblase 1 plays a role in maintaining lipid asymmetry especially during cellular activation or apoptosis.
Biological function summary

Scramblase 1 mediates phospholipid scrambling in response to increased intracellular calcium levels facilitating changes in cell membrane morphology. It performs this function independently and as part of specific protein complexes that regulate membrane curvature and vesicle fusion. The role of PLSCR1 extends beyond membrane activities; it has been linked to signal transduction and the immune response suggesting a broader impact on cellular dynamics.

Pathways

Scramblase 1 influences critical biological processes like blood coagulation and apoptosis. It interacts with proteins such as annexin V which stabilizes membrane phospholipids and regulates cell death pathways. In the coagulation cascade PLSCR1 has been shown to interact with factors involved in platelet activation demonstrating its regulatory role in maintaining hemostasis and tissue homeostasis.

Scramblase 1 has notable implications in cancer and autoimmune diseases. Altered expression or mutation of PLSCR1 links directly to the progression of certain cancers often associating with proteins like p53 which plays an important role in cell cycle regulation and tumor suppression. In autoimmune disorders Scramblase 1’s activity affects the balance of immune cell signaling potentially linking it with proteins involved in inflammatory responses such as cytokines. Understanding these connections provides insights into potential therapeutic targets and diagnostic markers for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer leaflet of the plasma membrane resulting in collapse of the phospholipid asymmetry which leads to phosphatidylserine externalization on the cell surface (PubMed : 10770950, PubMed : 18629440, PubMed : 23590222, PubMed : 23659204, PubMed : 24343571, PubMed : 24648509, PubMed : 29748552, PubMed : 32110987, PubMed : 8663431, PubMed : 9218461, PubMed : 9485382, PubMed : 9572851). Mediates calcium-dependent phosphatidylserine externalization and apoptosis in neurons via its association with TRPC5 (By similarity). Also exhibits magnesium-dependent nuclease activity against double-stranded DNA and RNA but not single-stranded DNA and can enhance DNA decatenation mediated by TOP2A (PubMed : 17567603, PubMed : 27206388). Negatively regulates FcR-mediated phagocytosis in differentiated macrophages (PubMed : 26745724). May contribute to cytokine-regulated cell proliferation and differentiation (By similarity). May play a role in the antiviral response of interferon (IFN) by amplifying and enhancing the IFN response through increased expression of select subset of potent antiviral genes (PubMed : 15308695). Inhibits the functions of viral transactivators, including human T-cell leukemia virus (HTLV)-1 protein Tax, human immunodeficiency virus (HIV)-1 Tat, human hepatitis B virus (HBV) HBx, Epstein-Barr virus (EBV) BZLF1 and human cytomegalovirus IE1 and IE2 proteins through direct interactions (PubMed : 22789739, PubMed : 23501106, PubMed : 25365352, PubMed : 31434743, PubMed : 35138119). Also mediates the inhibition of influenza virus infection by preventing nuclear import of the viral nucleoprotein/NP (PubMed : 29352288, PubMed : 35595813). Plays a crucial role as a defense factor against SARS-CoV-2 independently of its scramblase activity by directly targeting nascent viral vesicles to prevent virus-membrane fusion and the release of viral RNA into the host-cell cytosol (PubMed : 37438530).. (Microbial infection) Acts as an attachment receptor for HCV.
See full target information PLSCR1

Alternative Names

Phospholipid scramblase 1, PL scramblase 1, Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase, Mg(2+)-dependent nuclease, MmTRA1b, PLSCR1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Histochemistry and cell biology 143:381-96 PubMed25362260

2014

Phospholipid scramblase 1 (PLSCR1) in villous trophoblast of the human placenta.

Applications

Unspecified application

Species

Unspecified reactive species

Veronika M Berghold,Martin Gauster,Denise G Hemmings,Gerit Moser,Julia Kremshofer,Monika Siwetz,Monika Sundl,Berthold Huppertz
View all publications
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Product promise

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