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Rabbit Polyclonal SCRIBBLE antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Protein scribble homolog aa 1550 to C-terminus.

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Images

Western blot - Anti-SCRIBBLE antibody (AB154067), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRIBBLE antibody (AB154067), expandable thumbnail
  • Western blot - Anti-SCRIBBLE antibody (AB154067), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human Protein scribble homolog aa 1550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14160

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Scaffold protein involved in different aspects of polarized cell differentiation regulating epithelial and neuronal morphogenesis and T-cell polarization (PubMed:15182672, PubMed:16344308, PubMed:16965391, PubMed:18641685, PubMed:18716323, PubMed:19041750, PubMed:27380321). Via its interaction with CRTAM, required for the late phase polarization of a subset of CD4+ T-cells, which in turn regulates TCR-mediated proliferation and IFNG and IL22 production (By similarity). Plays a role in cell directional movement, cell orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Promotes epithelial cell layer barrier function via maintaining cell-cell adhesion (By similarity). Most probably functions in the establishment of apico-basal cell polarity (PubMed:16344308, PubMed:19041750). May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium (PubMed:16965391, PubMed:19041750). May regulate cell invasion via MAPK-mediated cell migration and adhesion (PubMed:18641685, PubMed:18716323). May play a role in exocytosis and in the targeting of synaptic vesicles to synapses (PubMed:15182672). Functions as an activator of Rac GTPase activity (PubMed:15182672).

Alternative names

Recommended products

Rabbit Polyclonal SCRIBBLE antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Protein scribble homolog aa 1550 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human Protein scribble homolog aa 1550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14160
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SCRIBBLE also known as SCRIB or Scribble homolog is a protein that mainly functions in cell polarity and adhesion. It has a mass of about 215 kDa. SCRIBBLE interacts with other cellular structures to maintain apical-basal polarity. Researchers observe its expression in several tissue types especially in epithelial tissues where maintaining cell polarity is important. SCRIBBLE acts as a scaffold that links signaling molecules and components of membranes facilitating the organization of molecular assemblies on the plasma membrane.

Biological function summary

SCRIBBLE contributes significantly to cell orientation and tissue architecture. It is a critical component of the Scribble complex which also includes the proteins LGL1 and DLG1. This complex modulates cell junctions and the establishment of cell polarity. SCRIBBLE plays roles in processes such as wound healing and neural growth by modulating cell migration. It also influences epithelial-mesenchymal transition (EMT) a process important in development and cancer metastasis.

Pathways

SCRIBBLE is integral in the regulation of the Hippo signaling pathway. Proper function of this pathway is necessary for controlling organ size and suppressing tumorigenesis. SCRIBBLE interacts with proteins such as YAP and TAZ which act as transcriptional co-activators within the Hippo pathway. Additionally SCRIBBLE is linked to the Wnt signaling pathway where it interacts with Disheveled proteins impacting processes such as cell fate and proliferation.

Associated diseases and disorders

SCRIBBLE is implicated in cancer progression particularly in breast and colorectal cancers. Disruption in SCRIBBLE's function or expression can lead to loss of cell polarity and increased invasiveness contributing to tumor development. In breast cancer its interaction with the protein LATS2 in the Hippo pathway is important for controlling cell proliferation and apoptosis. Abnormal SCRIBBLE expression is also associated with neural tube defects where proper cell polarity and signaling are required for normal neural development.

Product promise

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3 product images

  • Western blot - Anti-SCRIBBLE antibody (ab154067), expandable thumbnail

    Western blot - Anti-SCRIBBLE antibody (ab154067)

    5% SDS PAGE

    All lanes: Western blot - Anti-SCRIBBLE antibody (ab154067) at 1/1000 dilution

    All lanes: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 175 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRIBBLE antibody (ab154067), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRIBBLE antibody (ab154067)

    Immunohistochemical analysis of human colon carcinoma tissue staining SCRIBBLE at cytoplasm with ab154067 at 1/250 dilution.

    Antigen Retrieval: Trilogy™ EDTA based buffer, pH 8.0, 15min.

  • Western blot - Anti-SCRIBBLE antibody (ab154067), expandable thumbnail

    Western blot - Anti-SCRIBBLE antibody (ab154067)

    5% SDS PAGE

    All lanes: Western blot - Anti-SCRIBBLE antibody (ab154067) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Lane 2: JC (mouse mammary gland adenocarcinoma cell line) whole cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 175 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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