JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB125080

Anti-SCRIBBLE antibody [EPR4140(2)]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal SCRIBBLE antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CRIB1, KIAA0147, LAP4, SCRB1, VARTUL, SCRIB, Protein scribble homolog, Scribble, hScrib, Protein LAP4

3 Images
Flow Cytometry (Intracellular) - Anti-SCRIBBLE antibody [EPR4140(2)] (AB125080)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SCRIBBLE antibody [EPR4140(2)] (AB125080)

ab125080 at 1/100, staining SCRIBBLE in permeabilized 293T cells by intracellular flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).

Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (AB125080)
  • WB

Lab

Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (AB125080)

Lanes 1-2 : Merged signal (red and green). Green - ab125080 observed at 240 kDa. Red - loading control ab8245 observed at 36 kDa.

ab125080 Anti-SCRIBBLE antibody [EPR4140(2)] was shown to specifically react with SCRIBBLE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265190 (knockout cell lysate ab257660) was used. Wild-type and SCRIBBLE knockout samples were subjected to SDS-PAGE. ab125080 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

SCRIB knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human SCRIB (SCRIBBLE) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-scrib-scribble-knockout-hela-cell-line-ab265190'>ab265190</a>)

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 175 kDa

Observed band size: 240 kDa

false

Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (AB125080)
  • WB

Unknown

Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (AB125080)

All lanes:

Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 175 kDa

false

  • Carrier free

    Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4140(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCRIBBLE also known as SCRIB or Scribble homolog is a protein that mainly functions in cell polarity and adhesion. It has a mass of about 215 kDa. SCRIBBLE interacts with other cellular structures to maintain apical-basal polarity. Researchers observe its expression in several tissue types especially in epithelial tissues where maintaining cell polarity is important. SCRIBBLE acts as a scaffold that links signaling molecules and components of membranes facilitating the organization of molecular assemblies on the plasma membrane.
Biological function summary

SCRIBBLE contributes significantly to cell orientation and tissue architecture. It is a critical component of the Scribble complex which also includes the proteins LGL1 and DLG1. This complex modulates cell junctions and the establishment of cell polarity. SCRIBBLE plays roles in processes such as wound healing and neural growth by modulating cell migration. It also influences epithelial-mesenchymal transition (EMT) a process important in development and cancer metastasis.

Pathways

SCRIBBLE is integral in the regulation of the Hippo signaling pathway. Proper function of this pathway is necessary for controlling organ size and suppressing tumorigenesis. SCRIBBLE interacts with proteins such as YAP and TAZ which act as transcriptional co-activators within the Hippo pathway. Additionally SCRIBBLE is linked to the Wnt signaling pathway where it interacts with Disheveled proteins impacting processes such as cell fate and proliferation.

SCRIBBLE is implicated in cancer progression particularly in breast and colorectal cancers. Disruption in SCRIBBLE's function or expression can lead to loss of cell polarity and increased invasiveness contributing to tumor development. In breast cancer its interaction with the protein LATS2 in the Hippo pathway is important for controlling cell proliferation and apoptosis. Abnormal SCRIBBLE expression is also associated with neural tube defects where proper cell polarity and signaling are required for normal neural development.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Scaffold protein involved in different aspects of polarized cell differentiation regulating epithelial and neuronal morphogenesis and T-cell polarization (PubMed : 15182672, PubMed : 16344308, PubMed : 16965391, PubMed : 18641685, PubMed : 18716323, PubMed : 19041750, PubMed : 27380321). Via its interaction with CRTAM, required for the late phase polarization of a subset of CD4+ T-cells, which in turn regulates TCR-mediated proliferation and IFNG and IL22 production (By similarity). Plays a role in cell directional movement, cell orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Promotes epithelial cell layer barrier function via maintaining cell-cell adhesion (By similarity). Most probably functions in the establishment of apico-basal cell polarity (PubMed : 16344308, PubMed : 19041750). May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium (PubMed : 16965391, PubMed : 19041750). May regulate cell invasion via MAPK-mediated cell migration and adhesion (PubMed : 18641685, PubMed : 18716323). May play a role in exocytosis and in the targeting of synaptic vesicles to synapses (PubMed : 15182672). Functions as an activator of Rac GTPase activity (PubMed : 15182672).
See full target information SCRIB
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 13:e0197266 PubMed29768513

2018

Increased mitochondrial fission is critical for hypoxia-induced pancreatic beta cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Da Zhang,Yanfang Liu,Yao Tang,Xiaofeng Wang,Zhichao Li,Rui Li,Zhenyu Ti,Weidong Gao,Jigang Bai,Yi Lv
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB154067

Anti-SCRIBBLE antibody

Western blot - Anti-SCRIBBLE antibody (AB154067)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Cell Lines & Lysates

AB265190

Human SCRIB (SCRIBBLE) knockout HeLa cell line

Advanced Validation

Western blot - Human SCRIB (SCRIBBLE) knockout HeLa cell line (AB265190)

  • 0
  • 0
Primary Antibodies

AB176570

Anti-MRas antibody [EPR12457]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-MRas antibody [EPR12457] (AB176570)

  • 0
  • 0
Primary Antibodies

AB317826

Anti-ECT2 antibody [EPR29014-24] - BSA and Azide free

Recombinant|RabMAb

Western blot - Anti-ECT2 antibody [EPR29014-24] - BSA and Azide free (AB317826)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com