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Rabbit Recombinant Monoclonal SCRIBBLE antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (AB248114), expandable thumbnail
  • Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (AB248114), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (AB248114), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Scaffold protein involved in different aspects of polarized cell differentiation regulating epithelial and neuronal morphogenesis and T-cell polarization (PubMed:15182672, PubMed:16344308, PubMed:16965391, PubMed:18641685, PubMed:18716323, PubMed:19041750, PubMed:27380321). Via its interaction with CRTAM, required for the late phase polarization of a subset of CD4+ T-cells, which in turn regulates TCR-mediated proliferation and IFNG and IL22 production (By similarity). Plays a role in cell directional movement, cell orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Promotes epithelial cell layer barrier function via maintaining cell-cell adhesion (By similarity). Most probably functions in the establishment of apico-basal cell polarity (PubMed:16344308, PubMed:19041750). May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium (PubMed:16965391, PubMed:19041750). May regulate cell invasion via MAPK-mediated cell migration and adhesion (PubMed:18641685, PubMed:18716323). May play a role in exocytosis and in the targeting of synaptic vesicles to synapses (PubMed:15182672). Functions as an activator of Rac GTPase activity (PubMed:15182672).

Alternative names

CRIB1, KIAA0147, LAP4, SCRB1, VARTUL, SCRIB, Protein scribble homolog, Scribble, hScrib, Protein LAP4

Recommended products

Rabbit Recombinant Monoclonal SCRIBBLE antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR4140(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248114 is the carrier-free version of Anti-SCRIBBLE antibody [EPR4140(2)] ab125080.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SCRIBBLE also known as SCRIB or Scribble homolog is a protein that mainly functions in cell polarity and adhesion. It has a mass of about 215 kDa. SCRIBBLE interacts with other cellular structures to maintain apical-basal polarity. Researchers observe its expression in several tissue types especially in epithelial tissues where maintaining cell polarity is important. SCRIBBLE acts as a scaffold that links signaling molecules and components of membranes facilitating the organization of molecular assemblies on the plasma membrane.

Biological function summary

SCRIBBLE contributes significantly to cell orientation and tissue architecture. It is a critical component of the Scribble complex which also includes the proteins LGL1 and DLG1. This complex modulates cell junctions and the establishment of cell polarity. SCRIBBLE plays roles in processes such as wound healing and neural growth by modulating cell migration. It also influences epithelial-mesenchymal transition (EMT) a process important in development and cancer metastasis.

Pathways

SCRIBBLE is integral in the regulation of the Hippo signaling pathway. Proper function of this pathway is necessary for controlling organ size and suppressing tumorigenesis. SCRIBBLE interacts with proteins such as YAP and TAZ which act as transcriptional co-activators within the Hippo pathway. Additionally SCRIBBLE is linked to the Wnt signaling pathway where it interacts with Disheveled proteins impacting processes such as cell fate and proliferation.

Associated diseases and disorders

SCRIBBLE is implicated in cancer progression particularly in breast and colorectal cancers. Disruption in SCRIBBLE's function or expression can lead to loss of cell polarity and increased invasiveness contributing to tumor development. In breast cancer its interaction with the protein LATS2 in the Hippo pathway is important for controlling cell proliferation and apoptosis. Abnormal SCRIBBLE expression is also associated with neural tube defects where proper cell polarity and signaling are required for normal neural development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com