AB126331

Anti-SCRT1 antibody

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(1 Publication)

Rabbit Polyclonal SCRT1 antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Transcriptional repressor scratch 1.

View Alternative Names

Transcriptional repressor scratch 1, Scratch homolog 1 zinc finger protein, SCRT, Scratch 1, hScrt, SCRT1

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-SCRT1 antibody (AB126331)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SCRT1 antibody (AB126331)

Immunocytochemical analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cells labeling SCRT1 in the nuclear bodies with ab126331 at 2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRT1 antibody (AB126331)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRT1 antibody (AB126331)

Immunohistochemical analysis of human skeletal muscle tissue labeling SCRT1 with ab126331 at a 1/20 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRT1 antibody (AB126331)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRT1 antibody (AB126331)

Immunohistochemical analysis of human cerebellum tissue labeling SCRT1 in the nucleus of cells in the molecular layer with ab126331 at a 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRT1 antibody (AB126331)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRT1 antibody (AB126331)

Immunohistochemical analysis of human cerebral cortex tissue labeling SCRT1 in the nucleus of neurons with ab126331 at a 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRT1 antibody (AB126331)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRT1 antibody (AB126331)

Immunohistochemical analysis of mouse brain tissue labeling SCRT1 in the nucleus of neurons with ab126331 at a 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRT1 antibody (AB126331)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCRT1 antibody (AB126331)

Immunohistochemical analysis of mouse retina tissue labeling SCRT1 with ab126331 at a 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF

Immunogen

Recombinant Fragment Protein within Human Transcriptional repressor scratch 1. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCRT1 also known as Scratch family transcriptional repressor 1 is a zinc finger transcription factor with a molecular weight of approximately 40 kDa. It is expressed in the central nervous system particularly in areas associated with neuronal development and differentiation. The SCRT1 protein binds to specific DNA sequences modulating gene expression by repressing target genes involved in cellular processes.

Biological function summary

SCRT1 plays a role in regulating neuronal differentiation and migration during development. It is not part of a large protein complex but interacts with other transcription factors to modify transcriptional activities. SCRT1 achieves its biological function by repressing genes essential for the maintenance of neural progenitors thereby facilitating the transition to mature neuronal phenotypes.

Pathways

SCRT1 influences neural development pathways interacting with Notch signaling and other developmental pathways. Within these frameworks SCRT1 negatively regulates the expression of genes downstream of proteins like Hes1 and Ascl1 which are important for maintaining neuroprogenitor characteristics and promoting neuronal maturation. This tight regulation ensures proper neuronal development by controlling the balance between neural cell proliferation and differentiation.

Mutations or dysregulation of SCRT1 have connections to neurodevelopmental disorders such as intellectual disabilities and autism spectrum disorders. Additionally SCRT1 interacts with proteins like REST which is implicated in the pathogenesis of such conditions. Altered SCRT1 activity may contribute to the failure of neuronal cells to correctly differentiate leading to the manifestations seen in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional repressor that binds E-box motif CAGGTG. Can modulate the action of basic helix-loop-helix (bHLH) transcription factors, critical for neuronal differentiation.

See full target information Transcriptional repressor scratch 1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:681 PubMed39289348

2024

MANF serves as a novel hepatocyte factor to promote liver regeneration after 2/3 partial hepatectomy via doubly targeting Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyan Liang,Qiong Mei,Enguang He,Petek Ballar,Chuansheng Wei,Yue Wang,Yue Dong,Jie Zhou,Xiaofang Tao,Wenyan Qu,Mingxia Zhao,Goma Chhetri,Limeng Wei,Juntang Shao,Yujun Shen,Jun Liu,Lijie Feng,Yuxian Shen

View all publications

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Product promise

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