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AB105378

Anti-SCUBE2 antibody

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(2 Publications)

Rabbit Polyclonal SCUBE2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SCUBE2.

View Alternative Names

CEGP1, SCUBE2, Protein CEGP1, Scube/You

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SCUBE2 antibody (AB105378)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SCUBE2 antibody (AB105378)

Immunofluorescence of SCUBE2 in Daudi cells using ab105378 at 20 ug/ml.

Immunocytochemistry/ Immunofluorescence - Anti-SCUBE2 antibody (AB105378)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SCUBE2 antibody (AB105378)

ab105378 at 20 µg/ml staining SCUBE2 in Daudi cells by Immunocytochemistry

Western blot - Anti-SCUBE2 antibody (AB105378)
  • WB

Unknown

Western blot - Anti-SCUBE2 antibody (AB105378)

All lanes:

Western blot - Anti-SCUBE2 antibody (ab105378) at 1 µg/mL

Lane 1:

Daudi cell lysate without blocking peptide at 15 µg

Lane 2:

Daudi cell lysate with blocking peptide at 15 µg

Predicted band size: 109 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human SCUBE2. The exact immunogen used to generate this antibody is proprietary information.

Q9NQ36

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCUBE2 also known as Signal Peptide CUB Domain EGF-like 2 is a protein carrying a significant role in cellular processes. It has an approximate mass of 123 kDa and identifies as a secreted glycoprotein. SCUBE2 is expressed strongly in tissues such as the heart kidney and brain. Its structure includes several epidermal growth factor-like repeats and CUB domains which help in mediating its interactions in the cellular environment.
Biological function summary

The SCUBE2 protein functions as an important mediator in developmental signal transduction pathways. It does not form part of a well-defined complex but interacts with other proteins to modulate key developmental signals like Hedgehog and BMP pathways. SCUBE2 participates in cell-to-cell communication assisting in the regulation of embryonic development and tissue patterning. This protein's role is essential in ensuring proper growth and differentiation in those developing tissues.

Pathways

SCUBE2 actively engages in the Hedgehog and BMP signaling pathways. These pathways involve critical mediator proteins such as Sonic Hedgehog (SHH) and Bone Morphogenetic Proteins (BMPs) respectively. SCUBE2 interacts with these molecules to influence processes like cellular proliferation and differentiation. By participating in these pathways SCUBE2 influences developmental and cellular mechanisms ensuring proper signaling during morphogenesis and tissue development.

SCUBE2 shows significant associations with various cancers and developmental disorders. For instance alterations in SCUBE2 expression levels have been linked to breast cancer where it may affect cancer cell signaling through interactions with the BMP signaling pathway proteins such as BMP-2. It also associates with developmental disorders influencing the Hedgehog signaling pathway which involves proteins such as GLI1 and affecting proper embryonic development. Understanding SCUBE2's involvement in these diseases could provide new insights into therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lipid-binding protein required for SHH long-range signaling by binding to the dually lipid-modified SHH (ShhNp) and by promoting ShhNp mobilization, solubilization and release from the cell membrane (PubMed : 22677548, PubMed : 22902404). Acts by enhancing the proteolytic processing (shedding) of the lipid-modified N- and C- terminal of ShhNp at the cell surface (PubMed : 24522195). Synergizes with DISP1 to increase SHH secretion (PubMed : 22902404). Probable cell surface coreceptor for VEGFR2 involved in VEGFR2-mediated angiogenesis (PubMed : 27834687).
See full target information SCUBE2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 15:142 PubMed32293498

2020

Knockdown of long non-coding RNA PVT1 induces apoptosis of fibroblast-like synoviocytes through modulating miR-543-dependent SCUBE2 in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Junxia Wang,Xianghui Kong,Haijian Hu,Shunfang Shi

Molecular vision 22:1387-1404 PubMed28003730

2016

Accumulation of cholesterol and increased demand for zinc in serum-deprived RPE cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sanghamitra Mishra,Katherine Peterson,Lili Yin,Alan Berger,Jianguo Fan,Graeme Wistow
View all publications
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Product promise

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