Rabbit Polyclonal SCUBE3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SCUBE3.
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Publications
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- Stem cell research & therapy 14:1302023Epithelium-derived SCUBE3 promotes polarized odontoblastic differentiation of dental mesenchymal stem cells and pulp regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZijie Wang,Chuying Chen,Jiayi Zhang,Jiangdie He,Lin Zhang,Jiayuan Wu,Zhihui TianPubMed 37189178
- Cancer cell international 22:12022SCUBE3 downregulation modulates hepatocellular carcinoma by inhibiting CCNE1 via TGFβ/PI3K/AKT/GSK3β pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPan Xu,Aoran Luo,Chuan Xiong,Hong Ren,Liang Yan,Qiang LuoPubMed 34980127
- Cancer cell international 21:2682021SCUBE3 serves as an independent poor prognostic factor in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Huo,Xi He,Zhenwei Li,Fan Yang,Shengnan He,Ling Shao,Ye Hu,Siqi Chen,Ni XiePubMed 34006286
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SCUBE3. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IX30
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.00000-2.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Is a positive regulator of the BMP signaling pathway, required for proper chondrogenesis, osteogenesis and skeletal development. It acts as a coreceptor for BMP ligands, particularly BMP2 and BMP4, facilitating their interactions with BMP type I receptors (PubMed:33308444). It is required for ligand-induced recruitment of BMP receptors to lipid rafts (By similarity). Binds to TGFBR2 and activates TGFB signaling. In lung cancer cells, could serve as an endogenous autocrine and paracrine ligand of TGFBR2, which could regulate TGFBR2 signaling and hence modulate epithelial-mesenchymal transition and cancer progression.
Alternative names
CEGF3, SCUBE3
Recommended products
Rabbit Polyclonal SCUBE3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SCUBE3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SCUBE3. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IX30
- Purification technique
- Affinity purification Immunogen
- Specificity
At least two isoforms of SCUBE3 are known to exist. SCUBE3 antibody is predicted to not cross-react with other SCUBE members.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein SCUBE3 also known as Signal Peptide CUB domain and Epidermal Growth Factor-like domain-containing protein 3 is a member of the SCUBE family. It is characterized by its mass of approximately 110 kDa. SCUBE3 is primarily expressed in various tissues including kidney lung heart and bone. Structurally it has important domains such as CUB and EGF-like domains that assist in its interaction with different molecules in the extracellular environment.
- Biological function summary
SCUBE3 functions as a coreceptor playing a significant role in modulating developmental and morphogenetic processes. It is part of a complex involved in cell signaling enhancing interactions between growth factors and their receptors. Through these interactions SCUBE3 regulates cell proliferation differentiation and movement essential in embryonic development and tissue homeostasis.
- Pathways
SCUBE3 plays a role in critical signaling pathways such as the Hedgehog and transforming growth factor-beta (TGF-β) pathways. It interacts with proteins like Smoothened (Smo) in the Hedgehog pathway and TGF-β receptors influencing cellular responses. By modulating these pathways SCUBE3 contributes to signaling cascades that are important for organ development and tissue repair.
- Associated diseases and disorders
SCUBE3 relates to pulmonary fibrosis and cancer. It has a connection with proteins like fibroblast growth factor (FGF) in these conditions. In pulmonary fibrosis aberrant SCUBE3 activity influences fibroblast proliferation and extracellular matrix deposition. In cancer particularly in aggressive tumor types SCUBE3's role in enhancing growth factor signaling can contribute to uncontrolled cell growth and metastasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-SCUBE3 antibody (ab189955)
Lane 1: Western blot - Anti-SCUBE3 antibody (ab189955) at 1 µg/mL
Lane 2: Western blot - Anti-SCUBE3 antibody (ab189955) at 2 µg/mL
All lanes: Mouse kidney tissue lysate
Predicted band size: 109 kDa, 50 kDa
Observed band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCUBE3 antibody (ab189955)
Immunofluorescence staining of Human kidney tissue, labeling SCUBE3 using ab189955 at 20 ug/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCUBE3 antibody (ab189955)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling SCUBE3 using ab189955 at 5 μg/ml
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