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AB189955

Anti-SCUBE3 antibody

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(4 Publications)

Rabbit Polyclonal SCUBE3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SCUBE3.

View Alternative Names

CEGF3, SCUBE3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCUBE3 antibody (AB189955)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCUBE3 antibody (AB189955)

Immunofluorescence staining of Human kidney tissue, labeling SCUBE3 using ab189955 at 20 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCUBE3 antibody (AB189955)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCUBE3 antibody (AB189955)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling SCUBE3 using ab189955 at 5 μg/ml

Western blot - Anti-SCUBE3 antibody (AB189955)
  • WB

Supplier Data

Western blot - Anti-SCUBE3 antibody (AB189955)

Lane 1:

Western blot - Anti-SCUBE3 antibody (ab189955) at 1 µg/mL

Lane 2:

Western blot - Anti-SCUBE3 antibody (ab189955) at 2 µg/mL

All lanes:

Mouse kidney tissue lysate

Predicted band size: 109 kDa,50 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SCUBE3. The exact immunogen used to generate this antibody is proprietary information.

Q8IX30

Specificity

At least two isoforms of SCUBE3 are known to exist. SCUBE3 antibody is predicted to not cross-react with other SCUBE members.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein SCUBE3 also known as Signal Peptide CUB domain and Epidermal Growth Factor-like domain-containing protein 3 is a member of the SCUBE family. It is characterized by its mass of approximately 110 kDa. SCUBE3 is primarily expressed in various tissues including kidney lung heart and bone. Structurally it has important domains such as CUB and EGF-like domains that assist in its interaction with different molecules in the extracellular environment.
Biological function summary

SCUBE3 functions as a coreceptor playing a significant role in modulating developmental and morphogenetic processes. It is part of a complex involved in cell signaling enhancing interactions between growth factors and their receptors. Through these interactions SCUBE3 regulates cell proliferation differentiation and movement essential in embryonic development and tissue homeostasis.

Pathways

SCUBE3 plays a role in critical signaling pathways such as the Hedgehog and transforming growth factor-beta (TGF-β) pathways. It interacts with proteins like Smoothened (Smo) in the Hedgehog pathway and TGF-β receptors influencing cellular responses. By modulating these pathways SCUBE3 contributes to signaling cascades that are important for organ development and tissue repair.

SCUBE3 relates to pulmonary fibrosis and cancer. It has a connection with proteins like fibroblast growth factor (FGF) in these conditions. In pulmonary fibrosis aberrant SCUBE3 activity influences fibroblast proliferation and extracellular matrix deposition. In cancer particularly in aggressive tumor types SCUBE3's role in enhancing growth factor signaling can contribute to uncontrolled cell growth and metastasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Is a positive regulator of the BMP signaling pathway, required for proper chondrogenesis, osteogenesis and skeletal development. It acts as a coreceptor for BMP ligands, particularly BMP2 and BMP4, facilitating their interactions with BMP type I receptors (PubMed : 33308444). It is required for ligand-induced recruitment of BMP receptors to lipid rafts (By similarity). Binds to TGFBR2 and activates TGFB signaling. In lung cancer cells, could serve as an endogenous autocrine and paracrine ligand of TGFBR2, which could regulate TGFBR2 signaling and hence modulate epithelial-mesenchymal transition and cancer progression.
See full target information SCUBE3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 14:130 PubMed37189178

2023

Epithelium-derived SCUBE3 promotes polarized odontoblastic differentiation of dental mesenchymal stem cells and pulp regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Zijie Wang,Chuying Chen,Jiayi Zhang,Jiangdie He,Lin Zhang,Jiayuan Wu,Zhihui Tian

Cancer cell international 22:1 PubMed34980127

2022

SCUBE3 downregulation modulates hepatocellular carcinoma by inhibiting CCNE1 via TGFβ/PI3K/AKT/GSK3β pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Pan Xu,Aoran Luo,Chuan Xiong,Hong Ren,Liang Yan,Qiang Luo

Cancer cell international 21:268 PubMed34006286

2021

SCUBE3 serves as an independent poor prognostic factor in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Huo,Xi He,Zhenwei Li,Fan Yang,Shengnan He,Ling Shao,Ye Hu,Siqi Chen,Ni Xie

American journal of human genetics 99:1281-1291 PubMed27866707

2016

A Functional Variant Associated with Atrial Fibrillation Regulates PITX2c Expression through TFAP2a.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangchuan Ye,Nathan R Tucker,Lu-Chen Weng,Sebastian Clauss,Steven A Lubitz,Patrick T Ellinor
View all publications
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