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AB307723

Anti-SCXA antibody [EPR26997-33] - BSA and Azide free

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Rabbit Recombinant Monoclonal SCXA antibody. Carrier free. Suitable for IHC-P and reacts with Rat, Mouse samples.

View Alternative Names

BHLHA41, BHLHA48, SCXA, SCXB, SCX, Basic helix-loop-helix transcription factor scleraxis, Class A basic helix-loop-helix protein 41, Class A basic helix-loop-helix protein 48, bHLHa41, bHLHa48

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] - BSA and Azide free (AB307723)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] - BSA and Azide free (AB307723)

This data was developed using ab307722, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SCXA with ab307722 at 1/500 (0.908 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on adult mouse cerebrum.The section was incubated with ab307722 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] - BSA and Azide free (AB307723)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] - BSA and Azide free (AB307723)

This data was developed using ab307722, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SCXA with ab307722 at 1/500 (0.908 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on adult rat cerebrum.The section was incubated with ab307722 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] - BSA and Azide free (AB307723)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] - BSA and Azide free (AB307723)

This data was developed using ab307722, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat embryonic 14.5 t tissue labeling SCXA with ab307722 at 1/500 (0.908 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on tendons of embryonic 14.5 rat.The section was incubated with ab307722 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] - BSA and Azide free (AB307723)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCXA antibody [EPR26997-33] - BSA and Azide free (AB307723)

This data was developed using ab307722, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse embryonic 14.5 tissue labeling SCXA with ab307722 at 1/500 (0.908 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on tendons of embryonic 14.5 mouse (PMID : 10477299).The section was incubated with ab307722 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26997-33

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCXA also known as Scleraxis A is a transcription factor with a molecular mass of approximately 23 kDa. This protein plays a significant mechanical role in gene expression regulation particularly in connective tissues where it binds to specific DNA sequences. SCXA expression occurs in tendons ligaments and other fibrous tissues where it serves an essential function in development and homeostasis.
Biological function summary

The Scleraxis A transcription factor regulates the differentiation of progenitor cells into tenocytes the cells that form tendons. SCXA operates within a transcriptional complex that directs the expression of genes necessary for tendon extracellular matrix synthesis and organization. The regulation by SCXA ensures the structural integrity and function of tendons and connective tissues critical for movement and force transmission.

Pathways

SCXA participates in key developmental signaling processes including the TGF-beta signaling pathway and the Notch signaling pathway. These pathways influence various biological processes related to cellular differentiation and tissue development. SCXA interacts with other proteins such as Mohawk homeobox (MKX) which also functions in tendon development modulating the cellular responses through these pathways to impact the differentiation and maturation of connective tissue cells.

Aberrant SCXA expression or function links to tendon-related pathologies and fibrotic conditions. Research shows potential connections between SCXA and conditions like tendinopathy characterized by tendon degeneration and impaired healing. SCXA also interacts with proteins such as scleraxis B (SCXB) in the pathological context where disruptions in their activity may lead to changes in tendon structure and mechanics impacting tissue response to stress and injury.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.
See full target information SCX
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com