AB95074

Anti-SCYL1 antibody

Name: Anti-SCYL1 antibody (ab95074) | Abcam
Brand: Abcam Limited
SKU: AB95074
Price: 470 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal SCYL1 antibody. Suitable for WB, IP and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SCYL1 aa 750 to C-terminus.

View Alternative Names

CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP, HT019, SCYL1, N-terminal kinase-like protein, Coated vesicle-associated kinase of 90 kDa, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratoma-associated tyrosine kinase

2 Images

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Immunoprecipitation - Anti-SCYL1 antibody (AB95074)

Detection of SCYL1 by Western Blot of Immunprecipitate.
ab95074 at 1µg/ml staining SCYL1 in HeLa whole cell lysate immunoprecipitated using ab95074 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-SCYL1 antibody (ab95074)

Predicted band size: 90 kDa

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Unknown

Western blot - Anti-SCYL1 antibody (AB95074)

All lanes:

Western blot - Anti-SCYL1 antibody (ab95074) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 90 kDa

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Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human SCYL1 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96KG9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Elephant": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Opossum": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab95074 was affinity purified using an epitope specific to SCYL1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCYL1 also known as Scy1 Like Pseudokinase 1 is a pseudokinase with a molecular mass of approximately 95 kDa. It localizes primarily to the cytoplasm and is strongly expressed in the central nervous system liver and kidney. The protein possesses several phosphorylation sites and a characteristic N-terminal kinase-like domain although it lacks the typical catalytic activity of kinases. SCYL1 appears to be involved in cellular processes related to protein trafficking and stability.

Biological function summary

SCYL1 contributes to the sorting and transport of proteins between the Golgi apparatus and the endoplasmic reticulum. It interacts with coatomer complexes to facilitate vesicular trafficking ensuring proper cellular function and protein homeostasis. As part of the cellular transport machinery SCYL1 helps maintain the structural integrity of the Golgi complex and likely plays role in neurodevelopment due to its expression patterns and involvement in protein handling.

Pathways

SCYL1 influences the Golgi-to-ER transport pathways and is involved in vesicle formation and movement. It works alongside proteins like coatomer protein complex subunits to maintain efficient vesicular transport within the cell. Through these pathways SCYL1 connects to broader cellular functions like protein sorting and endosome-to-Golgi retrieval contributing to the systematic recycling and allocation of proteins across the cellular framework.

SCYL1 mutations link the protein to spinocerebellar ataxia type 21 and the hepatic disorder 3C syndrome. These conditions highlight the protein's connections to neurological and liver functions reflecting its significant role in these organs. Additionally interactions with proteins like TDP-43 suggest potential contributions to neurodegenerative processes when misregulated. Understanding SCYL1's role in these diseases provides insight into its importance for normal cellular functioning and the consequences of its dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum (PubMed : 18556652). Involved in the maintenance of the Golgi apparatus morphology (PubMed : 26581903). Has no detectable kinase activity in vitro (PubMed : 18556652).. Isoform 6 acts as a transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region.

See full target information SCYL1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:852 PubMed40731404

2025

PYGO1 drives gastric cancer progression via the ITGB1/CD47 axis and is therapeutically targeted by pentagalloylglucose.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjuan Jia,Yaling Li,Yan Li,Yonghong Li,Tao Qu,Zhuomin Fu,Yuanyuan Ma,Zhenhao Li,Wanxia Wang,Miao Yu,Xiaojie Jin,Xiaoling Gao,Yongqi Liu

Molecular biology of the cell 31:1963-1973 PubMed32583741

2020

SCYL1 arginine methylation by PRMT1 is essential for neurite outgrowth via Golgi morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Genki Amano,Shinsuke Matsuzaki,Yasutake Mori,Ko Miyoshi,Sarina Han,Sho Shikada,Hironori Takamura,Takeshi Yoshimura,Taiichi Katayama

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SCYL1 antibody

Immunoprecipitation - Anti-SCYL1 antibody (AB95074)

Western blot - Anti-SCYL1 antibody (AB95074)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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