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AB221195

Anti-SCYL2 antibody

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(1 Publication)

Rabbit Polyclonal SCYL2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SCYL2 aa 500 to C-terminus.

View Alternative Names

CVAK104, KIAA1360, SCYL2, SCY1-like protein 2, Coated vesicle-associated kinase of 104 kDa

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SCYL2 antibody (AB221195)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SCYL2 antibody (AB221195)

Immunofluorescent analysis of 4% PFA fixed, 0.25 Triton X-100 permeabilized MCF7 cells labeling SCYL2 with ab221195 at 1/1000 dilution, followed by secondary detection with Alexa Fluor 488 conjugated goat anti rabbit IgG at 1/1000 dilution (green). DAPI was used for nuclear counterstaining (blue).

Western blot - Anti-SCYL2 antibody (AB221195)
  • WB

Supplier Data

Western blot - Anti-SCYL2 antibody (AB221195)

All lanes:

Western blot - Anti-SCYL2 antibody (ab221195) at 1/1000 dilution

Lane 1:

293 cell lysate siRNA control

Lane 2:

293 cell lysate treated with SCYL2 siRNA

Predicted band size: 104 kDa

false

Western blot - Anti-SCYL2 antibody (AB221195)
  • WB

Supplier Data

Western blot - Anti-SCYL2 antibody (AB221195)

7.5% SDS gel

All lanes:

Western blot - Anti-SCYL2 antibody (ab221195) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

MCF7 cell lysate at 20 µg

Lane 3:

NIH 3T3 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 104 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SCYL2 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6P3W7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Purification notes
ab221195 is purified by salting-out and ion-exchange chromatography.
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCYL2 also known by the name SCY1-like 2 is a member of the SCY1-like family of proteins. It has a mass of approximately 110 kDa. SCYL2 is an accessory protein with kinase-like activity but lacks enzymatic function. The protein localizes mainly in the trans-Golgi network and the cytoplasm. It is expressed in various tissues including brain and heart indicating its potential roles in different organs and systems within the body.
Biological function summary

The human SCYL2 protein is involved in cellular trafficking and is part of the coatomer complex which assists in vesicle formation and transport. It helps in clathrin-mediated endocytosis which is important for internalizing molecules like lipids and proteins. SCYL2 interacts with other proteins such as clathrin heavy chain and auxilin demonstrating its role in maintaining cellular homoeostasis and membrane dynamics.

Pathways

SCYL2 participates in the endocytosis and intracellular transport processes. It plays a part in the AP-2 complex pathway which is essential for the vesicle docking and cargo selection. Additionally SCYL2 associates with huntingtin an important protein involved in cellular interactions vital for neuron function and integrity connecting its role to neurological processes.

SCYL2 shows associations with neurological conditions like Huntington's disease. Disruptions in its regulatory functions can affect protein transport and neuron stability potentially leading to neurodegenerative symptoms. SCYL2 also interacts with tau proteins which are relevant in Alzheimer's disease indicating its potential influence in tauopathy-related neuronal deterioration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the AP2-containing clathrin coat that may regulate clathrin-dependent trafficking at plasma membrane, TGN and endosomal system (Probable). A possible serine/threonine-protein kinase toward the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L-lysine has not been confirmed (PubMed : 15809293, PubMed : 16914521). By regulating the expression of excitatory receptors at synapses, plays an essential role in neuronal function and signaling and in brain development (By similarity).
See full target information SCYL2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 57:1177-1188 PubMed40451926

2025

Inhibition of serotonin-Htr2b signaling in skeletal muscle mitigates obesity-induced insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Suhyeon Park,Hyeongseok Kim,Soyeon Shin,Yeongmin Kim,Yunwon Kang,Hye-Na Cha,So-Young Park,Sangkyu Park,Chang-Myung Oh
View all publications
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Product promise

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