Anti-SDF1 antibody (ab9797) is a rabbit polyclonal antibody that is used to detect SDF1 in Western Blot, IHC-P, ELISA. Suitable for Human, samples.
- Over 90 publications
- Trusted since 2002
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- iScience 26:1085232023AID-induced CXCL12 upregulation enhances castration-resistant prostate cancer cell metastasis by stabilizing β-catenin expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Li,Jinfeng Fan,Zhiyan Zhou,Zhe Ma,Zhifei Che,Yaoxi Wu,Xiangli Yang,Peiyu Liang,Haoyong LiPubMed 38162032
- Journal of cancer research and clinical oncology 149:17943-179552023High density of CXCL12-positive immune cell infiltration predicts chemosensitivity and recurrence-free survival in ovarian carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhilipp Köhn,Alexandros Lalos,Alberto Posabella,Alexander Wilhelm,Athanasios Tampakis,Ercan Caner,Uwe Güth,Sylvia Stadlmann,Giulio C Spagnoli,Salvatore Piscuoglio,Sabine Richarz,Tarik Delko,Raoul A Droeser,Gad SingerPubMed 37966614
- Experimental & molecular medicine 55:1713-17192023Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Ram Lee,Seon-Yeong Lee,Jeong Won Choi,In Gyu Um,Hyun Sik Na,Jung Ho Lee,Mi-La ChoPubMed 37524866
- Frontiers in immunology 14:11883512023Integrative multi-omics analyses unravel the immunological implication and prognostic significance of CXCL12 in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi-Jie Gao,Zhou Fang,Jing-Ping Yuan,Sheng-Rong Sun,Bei LiPubMed 37564657
- BMC musculoskeletal disorders 24:2752023SDF-1α promotes subchondral bone sclerosis and aggravates osteoarthritis by regulating the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiqiang Meng,Lujun Xin,Bosheng FanPubMed 37038152
- Antioxidants (Basel, Switzerland) 12:2022Specificity Protein 1-Mediated Promotion of CXCL12 Advances Endothelial Cell Metabolism and Proliferation in Pulmonary Hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEvan R DeVallance,Christopher M Dustin,Daniel Simoes de Jesus,Imad Al Ghouleh,John C Sembrat,Eugenia Cifuentes-Pagano,Patrick J PaganoPubMed 36670936
- BMC endocrine disorders 22:2922022The prognostic significance of CXCR4 and SDF-1 in differentiated thyroid cancer depends on CD8+ density.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander Wilhelm,Isabelle Lemmenmeier,Alexandros Lalos,Alberto Posabella,Venkatesh Kancherla,Salvatore Piscuoglio,Tarik Delko,Markus von Flüe,Kathrin Glatz,Raoul André DroeserPubMed 36419107
- Cancer medicine 12:5953-59632022CXCL12 is expressed by skeletal muscle cells in tongue oral squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkira Yorozu,Shohei Sekiguchi,Akira Takasawa,Fumika Okazaki,Takeshi Niinuma,Hiroshi Kitajima,Eiichiro Yamamoto,Masahiro Kai,Mutsumi Toyota,Yui Hatanaka,Koyo Nishiyama,Kazuhiro Ogi,Hironari Dehari,Kazufumi Obata,Makoto Kurose,Atsushi Kondo,Makoto Osanai,Akihiro Miyazaki,Kenichi Takano,Hiromu SuzukiPubMed 36300800
- Molecular cancer 21:1322022Comprehensive characterization of the prostate tumor microenvironment identifies CXCR4/CXCL12 crosstalk as a novel antiangiogenic therapeutic target in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsabel Heidegger,Georgios Fotakis,Anne Offermann,Jermaine Goveia,Sophia Daum,Stefan Salcher,Asma Noureen,Hetty Timmer-Bosscha,Georg Schäfer,Annemiek Walenkamp,Sven Perner,Aleksandar Beatovic,Matthieu Moisse,Christina Plattner,Anne Krogsdam,Johannes Haybaeck,Sieghart Sopper,Stefanie Thaler,Markus A Keller,Helmut Klocker,Zlatko Trajanoski,Dominik Wolf,Andreas PircherPubMed 35717322
- Science advances 8:eabl80542022CXCL12/CXCR4-Rac1-mediated migration of osteogenic precursor cells contributes to pathological new bone formation in ankylosing spondylitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaowen Cui,Zihao Li,Siwen Chen,Xiang Li,Dongying Chen,Jianru Wang,Zemin Li,Wenjun Hao,Fangling Zhong,Kuibo Zhang,Zhaomin Zheng,Zhongping Zhan,Hui LiuPubMed 35385310
Key facts
- Isotype
- Unknown
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CXCL12 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P48061
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Reactivity data
Select an application| IHC-P | WB | sELISA | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Goat | Predicted | Predicted | Predicted |
| Recombinant full length protein - Human | Not recommended | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Goat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes To detect hSDF-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Goat | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 2 µg/mL | Notes (using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with ab84277 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF1 alpha. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Goat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner (PubMed:29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity).
Alternative names
SDF1, SDF1A, SDF1B, CXCL12, Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, IRH, hIRH, PBSF
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Anti-SDF1 antibody (ab9797) is a rabbit polyclonal antibody that is used to detect SDF1 in Western Blot, IHC-P, ELISA. Suitable for Human, samples.
- Over 90 publications
- Trusted since 2002
Key facts
- Isotype
- Unknown
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CXCL12 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P48061
- Carrier free
- Yes
- Purification technique
- Affinity purification Immunogen
- Light chain type
- unknown
- Reconstitution
- Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Anti-SDF1 antibody (ab9797) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), ELISA in Human, samples.
Trusted by the scientific community
Anti-SDF1 (ab9797) was first used in a scientific publication in 2002 and has been cited over 90 times in peer-reviewed journals.
Reviewed by scientists
Anti-SDF1 (ab9797) has over 10 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The stromal cell-derived factor 1 (SDF1) also known as C-X-C motif chemokine 12 (CXCL12) is a chemokine protein that is important in immunological responses and cellular signaling. This protein weighs approximately 8 kDa. SDF1 is largely expressed in bone marrow stroma liver and endothelium of various tissues positioning it as an integral player in cell migration and homing processes. The protein functions as a chemoattractant for lymphocytes promoting cellular trafficking and organ development.
- Biological function summary
SDF1 influences the migration and survival of hematopoietic progenitor cells. It plays a pivotal role in heart development angiogenesis and neuronal protein regulation. SDF1 binds with high affinity to its receptor CXCR4 forming a critical signal transduction complex that modulates cellular movement and growth responses. This interaction is important in the regulation of cell positioning and potential pathways of pathological changes.
- Pathways
SDF1 has an important role in the chemokine signaling pathway and is involved in the pathways controlling hematopoietic stem cell migration and homing. The interaction between SDF1 and CXCR4 triggers downstream signaling events engaging proteins like PI3K and MAPK which promote cell survival and proliferation. Furthermore the SDF1/CXCR4 axis is central to the vascular endothelial growth factor (VEGF) pathway facilitating angiogenesis and tissue repair mechanisms.
- Associated diseases and disorders
SDF1 relates closely to cancer metastasis and HIV infection. The SDF1/CXCR4 interaction acts as a co-receptor for HIV entry into host cells implicating it in viral pathogenesis. Overexpression of SDF1 and its binding partner CXCR4 contributes to tumor growth invasion and metastasis in various cancers by promoting angiogenesis and tumor cell migration. The targeting of the SDF1/CXCR4 axis holds therapeutic potential in cancer treatment and infectious disease management.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab9797)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SDF1 with ab9797 at 2 μg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab9797)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling SDF1 with ab9797 at 2 μg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
Sandwich ELISA - Anti-SDF1 antibody (ab9797)
ELISA - Anti-IL-1 beta antibody (ab9797)
Sandwich ELISA detecting SDF1 using ab9797 at a concentration of 2.0 μg/ml.
Western blot - Anti-SDF1 antibody (ab9797)
To detect human SDSF1 by Western Blot analysis, Anti-TNF alpha antibody ab9739 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant human SDF1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human SDF1, respectively.
Non-reducing conditions.
All lanes: Western blot - Anti-SDF1 antibody (ab9797)
Predicted band size: 10 kDa
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