AB9797

Anti-SDF1 antibody

Name: Anti-SDF1 antibody (ab9797) | Abcam
Brand: Abcam Limited
SKU: AB9797
Price: 495 USD
Availability: InStock
Rating: 4 (14 reviews)

(14 Reviews)

(107 Publications)

Anti-SDF1 antibody (ab9797) is a rabbit polyclonal antibody detecting SDF1 in Western Blot, IHC-P, ELISA. Suitable for Human,.

- Over 90 publications
- Trusted since 2002

View Alternative Names

SDF1, SDF1A, SDF1B, CXCL12, Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, IRH, hIRH, PBSF

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (AB9797)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (AB9797)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SDF1 with ab9797 at 2 μg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (AB9797)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling SDF1 with ab9797 at 2 μg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.

Supplier Data

Western blot - Anti-SDF1 antibody (AB9797)

To detect human SDSF1 by Western Blot analysis, ab9739 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant human SDF1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Lanes 1-11 : 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human SDF1, respectively.

Non-reducing conditions.

All lanes:

Western blot - Anti-SDF1 antibody (ab9797)

Predicted band size: 10 kDa

false

Sandwich ELISA - Anti-SDF1 antibody (AB9797)

sELISA

Supplier Data

Sandwich ELISA - Anti-SDF1 antibody (AB9797)

Sandwich ELISA detecting SDF1 using ab9797 at a concentration of 2.0 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

Unknown

Light chain type

unknown

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, sELISA, WB

applications

Immunogen

Recombinant Fragment Protein within Human CXCL12 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P48061

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Goat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "To detect hSDF-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "2 µg/mL", "sELISA-species-notes": "(using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with <a href='/en-us/products/primary-antibodies/biotin-sdf1-antibody-ab84277'>ab84277</a> as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF1 alpha." } } }

style

left-column

Product details

What is this antibody validated in?
Anti-SDF1 antibody (ab9797) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), ELISA in Human, samples.

Trusted by the scientific community
Anti-SDF1 (ab9797) was first used in a scientific publication in 2002 and has been cited over 90 times in peer-reviewed journals.

Reviewed by scientists
Anti-SDF1 (ab9797) has over 10 independent reviews from customers.

style

left-column

Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.

Purification technique

Affinity purification Immunogen

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The stromal cell-derived factor 1 (SDF1) also known as C-X-C motif chemokine 12 (CXCL12) is a chemokine protein that is important in immunological responses and cellular signaling. This protein weighs approximately 8 kDa. SDF1 is largely expressed in bone marrow stroma liver and endothelium of various tissues positioning it as an integral player in cell migration and homing processes. The protein functions as a chemoattractant for lymphocytes promoting cellular trafficking and organ development.

Biological function summary

SDF1 influences the migration and survival of hematopoietic progenitor cells. It plays a pivotal role in heart development angiogenesis and neuronal protein regulation. SDF1 binds with high affinity to its receptor CXCR4 forming a critical signal transduction complex that modulates cellular movement and growth responses. This interaction is important in the regulation of cell positioning and potential pathways of pathological changes.

Pathways

SDF1 has an important role in the chemokine signaling pathway and is involved in the pathways controlling hematopoietic stem cell migration and homing. The interaction between SDF1 and CXCR4 triggers downstream signaling events engaging proteins like PI3K and MAPK which promote cell survival and proliferation. Furthermore the SDF1/CXCR4 axis is central to the vascular endothelial growth factor (VEGF) pathway facilitating angiogenesis and tissue repair mechanisms.

SDF1 relates closely to cancer metastasis and HIV infection. The SDF1/CXCR4 interaction acts as a co-receptor for HIV entry into host cells implicating it in viral pathogenesis. Overexpression of SDF1 and its binding partner CXCR4 contributes to tumor growth invasion and metastasis in various cancers by promoting angiogenesis and tumor cell migration. The targeting of the SDF1/CXCR4 axis holds therapeutic potential in cancer treatment and infectious disease management.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Chemoattractant active on T-lymphocytes and monocytes but not neutrophils (PubMed : 18802065, PubMed : 39093700). Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis (PubMed : 8752281, PubMed : 18802065, PubMed : 39093700). Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for CXCL12/SDF-1 (PubMed : 16107333, PubMed : 19255243). Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV : ITGB3, ITGA4 : ITGB1 and ITGA5 : ITGB1 in a CXCR4-independent manner (PubMed : 29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase (PubMed : 18802065). Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins (PubMed : 16107333, PubMed : 18802065, PubMed : 19255243, PubMed : 39093700). CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase (PubMed : 18802065). Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1 (PubMed : 8752281). Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation (By similarity). Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity).. SDF-1-beta(3-72). Shows a reduced chemotactic activity.. SDF-1-alpha(3-67). Shows a reduced chemotactic activity (PubMed : 14525775). Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites (PubMed : 14525775).

See full target information CXCL12

style

left-column

Publications (107)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 82:280 PubMed40682610

2025

CXCL12/CXCR4 modulates macrophage efferocytosis to induce glomerular crescent formation and fibrosis via ELMO1/DOCK180/RAC1 signaling in ANCA-associated glomerulonephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Zilin Liu,Yongqi Deng,Xiaomei Song,Xin Cai,Huaying Xiong,Liwen Tan,Shengsen Wei,Qiulin Li,Xiong Wang,Wei Jiang,Yaxi Chen,Qiu Li,Mo Wang

iScience 28:111952 PubMed40060904

2025

Blockade of TSP-1/CD47 signal axis promotes donor hematopoietic engraftment by improving SEC/MK niche function.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Wang,Yan-Hou Liu,Ting Zhang,Xintong Hou,Yanbao Xin,Guang-Yao Xie,Wen-Jie Zhao,Xue Wang,Tianmeng Sun,Zheng Hu,Yong-Guang Yang

Cellular & molecular biology letters 30:24 PubMed40038579

2025

KLF5 enhances CXCL12 transcription in adipose-derived stem cells to promote endothelial progenitor cells neovascularization and accelerate diabetic wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Yunjia Xie,Xuejun Ni,Xiaofen Wan,Nating Xu,Lu Chen,Chensheng Lin,Xi Zheng,Beichen Cai,Qian Lin,Ruonan Ke,Tao Huang,Xuefeng Hu,Biao Wang,Xiuying Shan

Translational oncology 52:102282 PubMed39808844

2025

A practical distribution pattern of α-SMA-positive carcinoma associated fibroblasts indicates poor prognosis of patients with pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Li,Meilong Shi,Yang Wang,Penghao Li,Xiaoyi Yin,Guoxiao Zhang,Xiaochao Kang,Huan Wang,Suizhi Gao,Kailian Zheng,Xiaohan Shi,Xiongfei Xu,Yukun Zhou,Hui Jiang,Wei Jing,Shiwei Guo,Gang Jin

The journal of headache and pain 25:213 PubMed39627724

2024

Elevated plasma CXCL12 leads to pain chronicity via positive feedback upregulation of CXCL12/CXCR4 axis in pain synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Ze Leng,Mei-Jia Fang,Yi-Min Wang,Zhen-Jia Lin,Qian-Yi Li,Ya-Nan Xu,Chun-Lin Mai,Jun-Ya Wan,Yangyinhui Yu,Ming Wei,Ying Li,Yu-Fan Zheng,Kai-Lang Zhang,Ya-Juan Wang,Li-Jun Zhou,Zhi Tan,Hui Zhang

Regenerative therapy 26:415-424 PubMed39070123

2024

Enhancement of tracheal cartilage regeneration by local controlled release of stromal cell-derived factor 1α with gelatin hydrogels and systemic administration of high-mobility group box 1 peptide.

Applications

Unspecified application

Species

Unspecified reactive species

Kumiko Ogi Suzuki,Tatsuya Okamoto,Katsuto Tamai,Yasuhiko Tabata,Etsuro Hatano

Chronic obstructive pulmonary diseases (Miami, Fla.) 11:482-495 PubMed39012715

2024

Interleukin-17A Promotes Airway Remodeling in Chronic Obstructive Pulmonary Disease by Activating C-X-C Motif Chemokine Ligand 12 Secreted by Lung Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolu Chen,Liping Chen,Guanying Chen,Jiapei Lv,Jincong Wang,Wanjun Yu,Huaying Wang

Aging 16:10868-10881 PubMed38949514

2024

Needle-scalpel therapy inhibits the apoptosis of nucleus pulposus cells via the SDF-1/CXCR4 axis in a rat degenerative cervical intervertebral disc model.

Applications

Unspecified application

Species

Unspecified reactive species

Wenlong Yang,Muqing Liu,Qinran Sun,Lei Liu,Wenqing Wu,Fangming Liu,Zhizhen Liu

JCI insight 9: PubMed38716725

2024

Cross-species single-cell analysis uncovers the immunopathological mechanisms associated with IgA nephropathy progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xizhao Chen,Tiantian Wang,Lei Chen,Yinghua Zhao,Yiyao Deng,Wanjun Shen,Lin Li,Zhong Yin,Chaoran Zhang,Guangyan Cai,Min Zhang,Xiangmei Chen

iScience 26:108523 PubMed38162032

2023

AID-induced CXCL12 upregulation enhances castration-resistant prostate cancer cell metastasis by stabilizing β-catenin expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Li,Jinfeng Fan,Zhiyan Zhou,Zhe Ma,Zhifei Che,Yaoxi Wu,Xiangli Yang,Peiyu Liang,Haoyong Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SDF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (AB9797)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (AB9797)

Western blot - Anti-SDF1 antibody (AB9797)

Sandwich ELISA - Anti-SDF1 antibody (AB9797)

Key facts

Host species

Clonality

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (107)

Product promise